UniProt UniProt http://www.uniprot.org naslavsky-2004-4 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Molecular biology of the cell 2004 imex curation Only protein-protein interactions naslavsky-2004-1 in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Molecular biology of the cell 2004 imex curation Only protein-protein interactions naslavsky-2004-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Molecular biology of the cell 2004 imex curation Only protein-protein interactions naslavsky-2004-3 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Molecular biology of the cell 2004 imex curation Only protein-protein interactions naslavsky-2004-5 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Naslavsky N., Boehm M., Backlund PS., Caplan S. scaplan@unmc.edu Molecular biology of the cell 2004 imex curation Only protein-protein interactions rbns5_human Rabenosyn-5 RBSN 110 kDa protein FYVE finger-containing Rab5 effector protein rabenosyn-5 RAB effector RBSN ZFYVE20 Zinc finger FYVE domain-containing protein 20 protein protein human Homo sapiens Human MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDVKGQIKSLVQKAKKAKDRLLKREGDDRAESGTQGYESFSYGGVDPYMWEPQELGAVRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTESAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKESLSTHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIVKLYEKLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSKKILTLGLNQDPPPHPSNLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEEMERKRAVERQAALESQRRLEERQSGLASRAANGEVASLRRGPAPLRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD ehd1_human EH domain-containing protein 1 Testilin CDABP0131 EHD1 PAST PAST1 PAST homolog 1 protein protein human Homo sapiens Human MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE ehd2_human EH domain-containing protein 2 EHD2 PAST2 PAST homolog 2 protein protein human Homo sapiens Human MFSWLKRGGARGQQPEAIRTVTSALKELYRTKLLPLEEHYRFGAFHSPALEDADFDGKPMVLVAGQYSTGKTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMHGDTEGTVPGNALVVDPDKPFRKLNPFGNTFLNRFMCAQLPNQVLESISIIDTPGILSGAKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGHEDKIRVVLNKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFRDIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPSVFGKENKKKQLILKLPVIFAKIQLEHHISPGDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGPDEAMEDGEEGSDDEAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIWKLSDVDRDGMLDDEEFALASHLIEAKLEGHGLPANLPRRLVPPSKRRHKGSAE ehd3_human EH domain-containing protein 3 EHD3 EHD2 PAST3 PAST homolog 3 protein protein human Homo sapiens Human MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE e1b9v6_bovin RBSN protein protein bovin Bos taurus (Bovine) Bovine MASLDDPGEVREGFLCPLCLKDLQSFYQLQSHYEEEHSGEDRDVKGQIKSLVQKARKAKNRLLKREGDDRAESGTQGYESFSYGGVDPYMWEPQELGAMRSHLSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTESAKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKDSLSAHTSPSQSPNSVHGSRRGSISSVSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKERTPDIVRLYEKLRLCMEKVDQKAPEYIKMAASLNAGETTYSLEHAGDLRVEVQKVYELIDALSKKILTLDMKQDPPPHPNTLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKQKRKQEMEQKRILERQAALESQRRLEERRSDLASRATNGEVAPPRRGPAPLRKAEGWLPLSEGQRQSEDPDPLLQQIHNITSFIRQAKAAGRTDEVRTLQENLRQLQDEYDQQQTEKAIALSRRQAEEEDLQREQLQVLREREREREQGQAASLHTRTRSLDFREIRPFQLEPSREPRTHLAHALDLGSSPARSGTATKTSPPSSAPEPFRGWSGPPALGQEFLPQSTASPRSEAPSLNPFDEEEDLASPSAEDTANPPAPEPSLGPPACVLREYNPFEEEDEEDAVAGNPFTKPDGPAPNPFEEEDEHPPRTPASPPVPGNPFEEAPCTNPFEEESDGGQEGAEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELRRTLARQKGGTD ehd1-rbsn-4 2844820 N/A 2844825 unspecified role unspecified role prey prey N/A 2844826 unspecified role unspecified role bait bait eh_domain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false Q9H4M9:p.Trp485Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 ehd2-rbsn-1 2844820 N/A 2844827 unspecified role unspecified role bait bait N/A 2844825 unspecified role unspecified role prey prey physical association physical association false false false 2 ehd3-rbsn-1 2844820 N/A 2844828 unspecified role unspecified role bait bait N/A 2844825 unspecified role unspecified role prey prey physical association physical association false false false 2 ehd1-rbsn-1 2844821 N/A 2844829 unspecified role unspecified role prey prey cell lysate cell lysate bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 2844826 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1B ehd1-rbsn-2 2844822 N/A 2844829 unspecified role unspecified role prey prey cell lysate cell lysate bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 2844826 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1C ehd1-rbsn-3 2844823 N/A 2844825 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9H1K0:p.[Asn626Ala;Phe628Ala;Asn662Ala;Phe664Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9H1K0:p.[Asn626Ala;Phe628Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9H1K0:p.[Asn662Ala;Phe664Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 2844826 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1D and 3 ehd1-rbsn-5 2844824 N/A 2844825 unspecified role unspecified role neutral component neutral component N/A 2844826 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 4C