IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ strohner-2001-5 in vitro In vitro solid phase assay solid phase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Strohner R., Nemeth A., Jansa P., Hofmann-Rohrer U., Santoro R., Längst G., Grummt I. I.Grummt@DKFZ-Heidelberg.de The EMBO journal 2001 imex curation Only protein-protein interactions strohner-2001-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Strohner R., Nemeth A., Jansa P., Hofmann-Rohrer U., Santoro R., Längst G., Grummt I. I.Grummt@DKFZ-Heidelberg.de HeLa cell cDNA The EMBO journal 2001 imex curation Only protein-protein interactions strohner-2001-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Strohner R., Nemeth A., Jansa P., Hofmann-Rohrer U., Santoro R., Längst G., Grummt I. I.Grummt@DKFZ-Heidelberg.de The EMBO journal 2001 imex curation Only protein-protein interactions strohner-2001-4 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Strohner R., Nemeth A., Jansa P., Hofmann-Rohrer U., Santoro R., Längst G., Grummt I. I.Grummt@DKFZ-Heidelberg.de The EMBO journal 2001 imex curation Only protein-protein interactions strohner-2001-6 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Strohner R., Nemeth A., Jansa P., Hofmann-Rohrer U., Santoro R., Längst G., Grummt I. I.Grummt@DKFZ-Heidelberg.de The EMBO journal 2001 imex curation Only protein-protein interactions mbd2_mouse Methyl-CpG-binding domain protein 2 Mbd2 Methyl-CpG-binding protein MBD2 protein protein mouse Mus musculus Mouse MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAARGGGVCGRGRGRGRGRGRGRGRGRGRGRPQSGGSGLGGDGGGGAGGCGGGSGGGVAPRRDPVPFPSGSSGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNAVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKFTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEVDIDMDSGDEA gam12 nucleic acid nucleic acid synthetic construct synthetic construct GATCMECGAMECGAMECGAMECGAMECGAMECGAMECGAMECGAMECGAMECGAMECGAMECGATC MBD2 only binds the methylated oligonucleotide (all the C are methylated except for the first one and the last one) gac nucleic acid nucleic acid synthetic construct synthetic construct GATCCGACGACGACGACGACGACGACGACGACGACGACGATC baz2a_human Bromodomain adjacent to zinc finger domain protein 2A BAZ2A KIAA0314 TIP5 Transcription termination factor I-interacting protein 5 hWALp3 protein protein human Homo sapiens Human MEMEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGILNSAPHSSSTSHLHHPSVAYDCLWNYSQYPSANPGSNLKDPPLLSQFSGGQYPLNGILGGSRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQEVGSGIHPDEAAEKEMTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVPSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILSEDSLEPFNSLAPEPVSGGLYGIDDTELMGAEDKLPLEDSPVISALDCPSLNNATAFSLLADDSQTSTSIFASPTSPPVLGESVLQDNSFDLNNGSDAEQEEMETQSSDFPPSLTQPAPDQSSTIQLHPATSPAVSPTTSPAVSLVVSPAASPEISPEVCPAASTVVSPAVFSVVSPASSAVLPAVSLEVPLTASVTSPKASPVTSPAAAFPTASPANKDVSSFLETTADVEEITGEGLTASGSGDVMRRRIATPEEVRLPLQHGWRREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEGLQWVQLSAEEIPSRIQAITGKRGRPRNTEKAKTKEVPKVKRGRGRPPKVKITELLNKTDNRPLKKLEAQETLNEEDKAKIAKSKKKMRQKVQRGECQTTIQGQARNKRKQETKSLKQKEAKKKSKAEKEKGKTKQEKLKEKVKREKKEKVKMKEKEEVTKAKPACKADKTLATQRRLEERQRQQMILEEMKKPTEDMCLTDHQPLPDFSRVPGLTLPSGAFSDCLTIVEFLHSFGKVLGFDPAKDVPSLGVLQEGLLCQGDSLGEVQDLLVRLLKAALHDPGFPSYCQSLKILGEKVSEIPLTRDNVSEILRCFLMAYGVEPALCDRLRTQPFQAQPPQQKAAVLAFLVHELNGSTLIINEIDKTLESMSSYRKNKWIVEGRLRRLKTVLAKRTGRSEVEMEGPEECLGRRRSSRIMEETSGMEEEEEEESIAAVPGRRGRRDGEVDATASSIPELERQIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDRYRRRYWVLPYLAGIFVEGTEGNLVPEEVIKKETDSLKVAAHASLNPALFSMKMELAGSNTTASSPARARGRPRKTKPGSMQPRHLKSPVRGQDSEQPQAQLQPEAQLHAPAQPQPQLQLQLQSHKGFLEQEGSPLSLGQSQHDLSQSAFLSWLSQTQSHSSLLSSSVLTPDSSPGKLDPAPSQPPEEPEPDEAESSPDPQALWFNISAQMPCNAAPTPPPAVSEDQPTPSPQQLASSKPMNRPSAANPCSPVQFSSTPLAGLAPKRRAGDPGEMPQSPTGLGQPKRRGRPPSKFFKQMEQRYLTQLTAQPVPPEMCSGWWWIRDPEMLDAMLKALHPRGIREKALHKHLNKHRDFLQEVCLRPSADPIFEPRQLPAFQEGIMSWSPKEKTYETDLAVLQWVEELEQRVIMSDLQIRGWTCPSPDSTREDLAYCEHLSDSQEDITWRGRGREGLAPQRKTTNPLDLAVMRLAALEQNVERRYLREPLWPTHEVVLEKALLSTPNGAPEGTTTEISYEITPRIRVWRQTLERCRSAAQVCLCLGQLERSIAWEKSVNKVTCLVCRKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCTVCLAQQVEGEFTQKPGFPKRGQKRKSGYSLNFSEGDGRRRRVLLRGRESPAAGPRYSEEGLSPSKRRRLSMRNHHSDLTFCEIILMEMESHDAAWPFLEPVNPRLVSGYRRIIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHIMRRFFESRWEEFYQGKQANL oligonucleotide nucleic acid nucleic acid synthetic construct synthetic construct GTTCCTTTGAGGTCCGGTTCTTTTCGTTATGGGGTCATTT It harbors the upstream control element of the murine rDNA promoter ttf1_mouse Transcription termination factor 1 Ttf1 RNA polymerase I termination factor Transcription termination factor I protein protein mouse Mus musculus Mouse MKGGTSKFKTHTETLYKKKKWSSVSEKRPQKCPSQCLESKQPQVSVLGKRRRASQTPAQETLESEWPQKAKRKKRRREPQTPAQETLESEWPQKAKKKKRRGEPQTPTQESLESEQPPVSLLGKRRRESQTPAQENSESEQPRKAKRRRKKRKGSQQPTSSLLKTPETFLKAKKTTSAHKKKKNSVLEVDMETGIILVDKENMENLLETSRKDVDIVYVDMSKGQRSAKVRETGELPAAKPQEHGCRELLGDVRSRKKQKHLQKVAPWDVVQGSQPESISLPPSEPLSSEDLEGKSTEAAVFCKKKSKKNVFRSQELEPIPDSLDDSETISERLDSTHHGGAVGAGEECESTKESHSIKKKSKKKKHKSVALATSSDSASVTDSKAKNALVDSSEGSGAVREEDVDHRPAEAEAQACSTEKHREAMQRLEPTHEEESNSESASNSAARHISEDRRESDDSDVDLGSAVRQLREFIPDIQERAATTIRRMYRDDLGRFKEFKAQGVAIRFGKFSAKENKQIEKNVQDFLSLTGIESADKLLYTDRYPEEKTLITNLKRKHAFRLHIGKGIARPWKLVYYRAKKIFDVNNYKGRYNEEDTKKLKAYHSLHGNDWKKIGAMVARSSLSVALKFSQIGGTRNQGAWSKAETQRLIKAVEDVILKKMSPQELRELDSKLQEDPEGRLSIVREKLYKGISWVEVEARVETRNWMQCKSKWTEILTKRMTHGGFVYRGVNALQAKITLIERLYELNVNDANEIDWEDLASAIGDVPPPFVQAKFYKLKAACVPFWQKKTFPEIIDYLYKNSLPLLKEKLDKKMKKKDGQIQTPAAPKQDFLFKDIFHCDDDSDEGSPEEPSASDVQ smca5_mouse SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 Smarca5 Snf2h Sucrose nonfermenting protein 2 homolog protein protein mouse Mus musculus Mouse MSSAVEPPPPPPPESAPSKPSAAGAGGSSSGNKGGPEGGAAPAAPCAAGSGPADTEMEEVFDHGSPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRVKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKKWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQREWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDKLLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTRAGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSIVIQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSLRNFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRSPDLPNAAQAQKEEQLKIDEAEPLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL baz2a_mouse Bromodomain adjacent to zinc finger domain protein 2A Baz2a Kiaa0314 Tip5 Transcription termination factor I-interacting protein 5 protein protein mouse Mus musculus Mouse MEMEANDHFNFTGLPPAPAASGLKPSPSSGEGLYTNGSPMNFPQQGKSLNGDVNVNGLSTVSHTTTSGILNSAPHSSSTSHLHHPNVAYDCLWNYSQYPSANPGNNLKDPPLLSQFPGGQYPLNGILGGNRQPSSPSHNTNLRAGSQEFWANGTQSPMGLNFDSQELYDSFPDQNFEVMPNGPPSFFTSPQTSPMLGSSIQTFAPSQDVSSDIHPDEAAEKELTSVVAENGTGLVGSLELEEEQPELKMCGYNGSVSSVESLHQEVSVLVPDPTVSCLDDPSHLPDQLEDTPILSEDSLEPFDSLAAEPVSGSLYGIDDAELMGAEDKLPLEGNPVISALDCPALSNANAFSLLADDSQTSASIFVSPTSPPVLGESVLQDNSFGLNSCSDSEQEEIETQSSNFQRPLTEPAPDQPPSTQLHPAVSPTASPAASLTASAEISPAVSPVASSPVPPEVFVAVSPASSPALPAISLEASMTTPVTSPQGSPEPSPAAAFQTVSPARKNVSSAPKARADREETTGGAVAVSGSGDVLKRRIATPEEVRLPLQHGWRREVRIKKGSHRWQGETWYYGPCGKRMKQFPEVIKYLSRNVVHSVRREHFSFSPRMPVGDFFEERDTPEGLQWVQLSAEEIPSRIQAITGKRGRPRNNEKAKNKEVPKVKRGRGRPPKIKMPELLNKTDNRLPKKLETQEILSEDDKAKMTKNKKKMRQKVQRGESQTPVQGQARNKRKQDTKSLKQKDTKKKLKAEKEKMKTKQEKLKEKVKREKKEKVKAKGKEGPRARPSCRADKTLATQKRLEEQQRQQAILEEMKKPTEGMCLSDHQPLPDFTRIPGLTLSSRAFSDCLTIVEFLHSFGKVLGFDLTKDVPSLGVLQEGLLCQGDSLDKVQDLLVRLLKAALHDPGLPPYCQSLKILGEKMSEIPLTRDNVSEILRCFLMAYRVEPPFCDSLRTQPFQAQPPQQKAAILAFLVHELNSSTIIINEIDKTLESVSSCRKNKWIVEGRLRRLKTALAKRTGRPEVMMEGAEDGLGRRRSSRIMEETSGIEEEEEEENTTAVHGRRGRKEGEIDVAASSIPELERHIEKLSKRQLFFRKKLLHSSQMLRAVSLGQDRYRRHYWVLPYLAGIFVEGSEGSTVTEDEIKQETESLMEVVTSTPSSARASVKRELTGSNASTSPARSRGRPRKPKPGSLQPQHLQSTIRECDSEQAQTQVHPEPQPQLQAPTQPHLQPSSGFLEPEGSPFSLGQSQHDLSQSAFLSWLSQTQSHNSLLSSSVLTPDSSPGKLDSAPSQSLEEPEPDEAQSCPGPQGPWFNFSAQIPCDAAPTPPPAVSEDQPTPSLQLLASSKPMNTPGAANPCSPVQLSSTHLPGGTPKRLSGDSEEMSQSPTGLGQPKRRGRPPSKFFKQVEQHYLTQLTAQPIPPEMCSGWWWIRDPETLDVLLKALHPRGIREKALHKHLSKHKDFLQEVCLQPLTDPIFEPNELPALEEGVMSWSPKEKTYETDLAVLQWVEELEQRVVLSDLQIRGWTCPTPDSTREDLTYCEHLPDSPEDIPWRGRGREGTVPQRQNNNPLDLAVMRLAVLEQNVERRYLREPLWAAHEVVVEKALLSTPNGAPDGTSTEISYEITPRVRVWRQTLERCRSAAQVCLCMGQLERSIAWEKSVNKVTCLVCRKGDNDEFLLLCDGCDRGCHIYCHRPKMEAVPEGDWFCAVCLSQQVEEEYTQRPGFPKRGQKRKSSFPLTFPEGDSRRRMLSRSRDSPAVPRYPEDGLSPPKRRRHSMRSHHSDLTFCEIILMEMESHDAAWPFLEPVNPRLVSGYRRVIKNPMDFSTMRERLLRGGYTSSEEFAADALLVFDNCQTFNEDDSEVGKAGHVMRRFFESRWEEFYQGKQANL mbd2-1 1936974 N/A 1936979 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) N/A 1936980 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false synthetic construct synthetic construct direct interaction direct interaction false false false Fig. 4B MBD2 only binds the oligonucleotide when it is methylated (GAM12) baz2a-1 1936974 N/A 1936981 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false synthetic construct synthetic construct N/A 1936982 unspecified role unspecified role bait bait TAM domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig.4B DNA methylation doesn't alter the DNA binding properties of TIP5 baz2a-oligonucleotide-1 1936974 N/A 1936982 unspecified role unspecified role bait bait TAM domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1936983 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false synthetic construct synthetic construct direct interaction direct interaction false false false Fig. 4C If TAM domain was fused to two AT-hooks (510aa-723aa) the DNA binding efficiency increased by almost two orders of magnitude. baz2a-ttf1-1 1936975 N/A 1936984 unspecified role unspecified role bait bait N/A 1936982 unspecified role unspecified role prey prey physical association physical association false false false baz2a-ttf1-3 1936976 N/A 1936982 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1936984 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false Fig. 3A baz2a-ttf1-4 1936977 N/A 1936982 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1936984 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse Mus musculus Mouse direct interaction direct interaction false false false Fig. 3B baz2a-smarca5-1 1936978 N/A 1936985 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 1936986 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false Fig.5D