IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xu-2001-2 caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu L., Fong Y., Strome S., Xu L., Fong Y., Strome S. sstrome@bio.indiana.edu Proceedings of the National Academy of Sciences of the United States of America 2001 imex curation Only protein-protein interactions imex curation xu-2001-3 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Xu L., Fong Y., Strome S., Xu L., Fong Y., Strome S. sstrome@bio.indiana.edu Proceedings of the National Academy of Sciences of the United States of America 2001 imex curation Only protein-protein interactions imex curation xu-2001-4 caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Xu L., Fong Y., Strome S., Xu L., Fong Y., Strome S. sstrome@bio.indiana.edu Proceedings of the National Academy of Sciences of the United States of America 2001 imex curation Only protein-protein interactions imex curation mes6_caeel Polycomb protein mes-6 mes-6 C09G4.5 Maternal-effect sterile protein 6 ESC homolog protein protein caeel Caenorhabditis elegans MEHTKKFKSLNLHGFDRSTEDYGKRPFVLTAKLLEDQKKAIYGCAFNQYAGIDEEQAVATVGGSFLHMYSVPIDINNIELQWSCNFPTDKSSKVEREESLFTVTWCYDTYEAENDRNPFKVVTGGTLGHIYVIDYVSRKLSNRLRSVGWEINDIRTCPANSNLIVCASSDQSIRIHHIRNEACLIVIGGLECHAGTILSVDWSTDGDFILSCGFDHQLMEWDLSVKQVKEHLERACKALHQDKINVLTQSQDIPYVSKGTMRKSAVSRNIPDKEEDQLLELHRELIPRPSCLLPIYTPSSVSTDMHSDYVDCIRFLIGTNYALSKGCGNEKAIHFWRFGPPKGEVENRIHGNVLRPKSCTTKFRTMNVPSGSAWFIKFAVDPRRRWLVCGGAGGSVMFFDLRNNEETNPTHTCSVGSRTVRQASFSTCGRFLVLVTDEGFVCRFDRVSASVDAKDLAKF mes3_caeel Polycomb protein mes-3 mes-3 F54C1.3 Maternal-effect sterile protein 3 protein protein caeel Caenorhabditis elegans MTPATAEVKVGGRKRKNDEPVFIKTKQSRSARREEEKENLLNKSLPSTPTSSEAGSSRESSNPVTSSSRRKNPPTKLENIQKTLPTCSDGLEIRNYVKKYGLPEDNKFLVRNVFDKQLLFGKKYVCRRRVIKSIDEFFPRLKTDAHRENGLYNFCTLQFMKISSWGSSMDEKIYVTSAAIVQSYVIIVDDNDSVTCIENGISVIPMANRTLPSISYNSSNVKNITLTGPELKKAKNVYLAVIVKRKTPKLGENRVGTRGKSTRASFETAASSENFQQLVRFGCTLMYDKDAEINCLNDGVQRIILLDREEKPAYLKIFGKADEPELENAWMNNVKHLEFISDTNYNKEDSHLARLIFSSNTHNYFDRPDISKFSPWQPRSRRTTLKSGISVEPGTSCDEDCGSQYEDTITLKQLLLGTKKLCGRRKRHLTRLTSRWPCFSVDPTKINSYFNETGVTLLENSCSYRPGTQRSIPIKPTRVIRVREETPVSVPIEVITIEDDSDDSPCFSARNRAPGGTRKYGLDFIQSSGHMPYIRYVYMNRANDLVPQLNGEENGKIEGLMEAYPMNNAHMYSDIEFLRKEKLNLPKNAQKYGEMTILDYPLTKTETDEYNKKMSVMNVKDFAVKPRKPIPTVGNTTLSLDLEGCPISTFSEIFFPSGKTELFNILCRHFYSEAGQRPGCFSAQWKSRVLTGFINKYHQYIFDMKLNSLFEKQINLIAMVSPDWKLEDFELPMVRYKQLKKEWKNRQRDIPSSN mes2_caeel Histone-lysine N-methyltransferase mes-2 E(z) homolog Maternal-effect sterile protein 2 R06A4.7 mes-2 protein protein caeel Caenorhabditis elegans MSNSEPSTSTPSGKTKKRGKKCETSMGKSKKSKNLPRFVKIQPIFSSEKIKETVCEQGIEECKRMLKGHFNAIKDDYDIRVKDELDTDIKDWLKDASSSVNEYRRRLQENLGEGRTIAKFSFKNCEKYEENDYKVSDSTVTWIKPDRTEEGDLMKKFRAPCSRIEVGDISPPMIYWVPIEQSVATPDQLRLTHMPYFGDGIDDGNIYEHLIDMFPDGIHGFSDNWSYVNDWILYKLCRAALKDYQGSPDVFYYTLYRLWPNKSSQREFSSAFPVLCENFAEKGFDPSSLEPWKKTKIAEGAQNLRNPTCYACLAYTCAIHGFKAEIPIEFPNGEFYNAMLPLPNNPENDGKMCSGNCWKSVTMKEVSEVLVPDSEEILQKEVKIYFMKSRIAKMPIEDGALIVNIYVFNTYIPFCEFVKKYVDEDDEESKIRSCRDAYHLMMSMAENVSARRLKMGQPSNRLSIKDRVNNFRRNQLSQEKAKRKLRHDSLRIQALRDGLDAEKLIREDDMRDSQRNSEKVRMTAVTPITACRHAGPCNATAENCACRENGVCSYMCKCDINCSQRFPGCNCAAGQCYTKACQCYRANWECNPMTCNMCKCDAIDSNIIKCRNFGMTRMIQKRTYCGPSKIAGNGLFLLEPAEKDEFITEYTGERISDDEAERRGAIYDRYQCSYIFNIETGGAIDSYKIGNLARFANHDSKNPTCYARTMVVAGEHRIGFYAKRRLEISEELTFDYSYSGEHQIAFRMVQTKERSEKPSRPKSQKLSKPMTSE mes_2-mes_3-1 1923154 N/A 1923157 unspecified role unspecified role prey prey N/A 1923158 unspecified role unspecified role prey prey N/A 1923159 unspecified role unspecified role bait bait association association false false false Fig 1a, 1B The anti-MES-2 antibodies were crosslinked to Protein A-agarose beads. mes_6-mes_2-1 1923154 N/A 1923157 unspecified role unspecified role bait bait N/A 1923158 unspecified role unspecified role prey prey N/A 1923159 unspecified role unspecified role prey prey association association false false false Fig 1C mes_2-mes_6-2 1923154 N/A 1923157 unspecified role unspecified role prey prey N/A 1923159 unspecified role unspecified role bait bait physical association physical association false false false The interaction between MES-2 and MES-6 is stable under high salt conditions. Participants were identified by a mix of 2 antibodies, and by molecular weight separation. Fig. 4 mes_2-mes_6-1 1923155 N/A 1923157 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1923159 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 2A Radiolabeled MES-6 was also precipitated by anti-MES-2 in the presence of cold MES-2 mes_6-mes_2-2 1923155 N/A 1923157 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 1923159 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig. 3B mes_2-mes_6-3 1923156 N/A 1923158 unspecified role unspecified role neutral component neutral component N/A 1923159 unspecified role unspecified role neutral component neutral component physical association physical association false false false Mes-6 is assumed, but not demonstrated, to be present. Fig. 5