IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lorenz-1998-2 chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lorenz E., Alekseev AE., Krapivinsky GB., Carrasco AJ., Clapham DE., Terzic A. 125[I]-azidoglyburide is a sulfonylurea that binds to the Kcnj11-Sur1 complex and was used to visualise both complex participants. It doesn't bind to Sur1 alone as no band is visible in the Kcnj11 peptide competition assay (Fig 1b, lane 2). Species were assigned according to previous papers: ABCC8: PMID 9182806 (Ref 10), Clement et al 1997 (Bryan lab): specifically state they used a hamster construct. KCNJ11: PMID 9202152 (Ref 24), Inagaki et al 1997 (Seino lab): specifically state they used a mouse construct. terzic.andre@mayo.edu Molecular and cellular biology 1998 Only protein-protein interactions imex curation imex curation lorenz-1998-1 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight by staining molecular weight estimation by staining weight by staining Lorenz E., Alekseev AE., Krapivinsky GB., Carrasco AJ., Clapham DE., Terzic A. Species were assigned according to previous papers: ABCC8: PMID 9182806 (Ref 10), Clement et al 1997 (Bryan lab): specifically state they used a hamster construct. KCNJ11: PMID 9202152 (Ref 24), Inagaki et al 1997 (Seino lab): specifically state they used a mouse construct. terzic.andre@mayo.edu Molecular and cellular biology 1998 Only protein-protein interactions imex curation imex curation kcj11_mouse ATP-sensitive inward rectifier potassium channel 11 Kcnj11 Inward rectifier K(+) channel Kir6.2 Potassium channel, inwardly rectifying subfamily J member 11 protein protein mouse Mus musculus Mouse MLSRKGIIPEEYVLTRLAEDPAEPRYRTRERRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMVWWLIAFAHGDLAPGEGTNVPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNGIFLVAPLIIYHVIDSNSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDRSLLDALTLASSRGPLRKRSVAVAKAKPKFSISPDSLS mint This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium g3i1t3_crigr ATP-sensitive inward rectifier potassium channel 11 Inward rectifier K(+) channel Kir6.2 Potassium channel, inwardly rectifying subfamily J member 11 I79_017352 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster VDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSFPNLLCFPTDIGLIPVKSSPEEPLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSVSSGPIQKPGIFISHVKPGSLSAEVGLETGDQIVEVNGIDFSNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEEIMEEEEKFKKQWEEDWGSKEQLILPKTITAEVHPTPLRKPKYDQGVEAKLEPADHLDGNTEKRGPQKGAEKKKAKHDSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKTQYWVERLCQTRLQQISSAENEIAETFCPSPKPPRGPGVSTISKPVMVHQEPNFIYRPAVKSEVLLVFVMCLSGFYILKPFLCFQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDLLLKSGERAQPDSILESRWGRQRSQVHIHHSTWLHFPGHNLRWILTFILLFVLVCEIAEGILSDGVTESRHLHLYMPAGMAFLAAVTSVVYYHNIETSNFPKLLIALLIYWTLAFITKTIKFVKFYDHAIGFSQLRFCLTGLLRYIFFKTPREVKPPEDLQDLGVRFLQPFVNLLSKGTYWWMNAFIKTAHKKPIDLRAIGKLPIAMRALTNYQRLCEAFDAQARKDMQSPQGARAIWRALCHAFGRRLILSSTFRILADLLGFAGPLCIFGIVDHLGKEHHVFQPKTKIYNKIMHLSTSNLSMGEMTAGQICNLVAIDTNQLMWFFFLCPNLWAMPVQIIVGVILLYYILGVSALIGAAVIILLAPVQYFVATKLSQAQRSTLEYSNERLKQTNEMLRGIKLLKLYAWENIFCSRVEMTRRKEMTSLRAFAVYTSISIFMNTAIPIAAVLITFVGHVSFFKESDFSPSVAFASLSLFHILVTPLFLLSSVVRSTVKALVSVQKLSEFLSSAEIREEQCAPREPAPQGQASKYQAVPLKVVNRKRPAREEVQDLLGPLQRLAPSMDGDADNFCVQLLPSIATGQLTMIVGQVGCGKSSLLLATLGEMQKVSGAVFWNSIPERETAAGSDIRSRGPVAYASQKPWLLNATVEENITFESPFNKQRYKKVIEACSLQPDIDILPHGDQTQIGERGINLSGGQRQRISVARALYQHTNVVFLDDPFSALDVHLSDHLMQAPILELLRDDKRTVVLVTHKLQYLPHADWIIAMKDGTIQREGTLKDFQRSECQLFEHWKTLMNRQDQELEKETVMERKASEPSQGLPRAMSSRDGLLLDEEEEEEEAAESEEDDNLSSVLHQRAKIPWKACTKYLSSAGILLLSLLVFSQLLKHMVLVAIDYWLAKWTDSVLVLSPAARNCSLSQECALDQSVYAMVFTVLCSLGIVLCLVTSVTVEWTGLKVAKRLHRSLLNRIILAPMRLEAGGVSVCRSALIQEAGRIDIYSTDPHIPSTLECLSRSTLLCVSALTVISYVTPVFLVALLPLAVVCYFIQKYFRVASRDLQQLDDTTQLPLLSHFAETVEGLTTIRAFRYEARFQQKLLEYTDSNNIASLFLTAANRWLEVRMEYIGACVVLIAAATSISNSLHGELSAGLVGLGLTYALMVSNYLNWMVRNLADMEIQLGAVKRIHALLKTEAENYEGLLGHSAVTSHTCAHPRSQCCDLPHLCTPQGHSVPLLLCFQIGICGRTGSGKSSFSLAFFRMVDMFEGRIIIDGIDIAKLPLHTLRSRLSIILQDPVLFSGTIRFNLDPEKKCSYSTLWEALEIAQLKLVVKALPGGLDAIITEGGENFSQGQRQLFCLARAFVRKTSIFIMDEATASIDMATHRVHTILSADLVMVLKRGAILEFDKPETLLSQKDSVFASFVRADKRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMVWWLLPFSHGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVITLRHGRLCFMLRVGDLRKSMIISATIHMQVVPLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEKPWGQRFVPIVAEEDGRYSVDYSKFGNTVKVPTPLCTARQLDEDRSLLDALTLASTRGPLRKRSVAVAKAKPKFSISPDSLS kcj11_human ATP-sensitive inward rectifier potassium channel 11 KCNJ11 IKATP Inward rectifier K(+) channel Kir6.2 Potassium channel, inwardly rectifying subfamily J member 11 protein protein human Homo sapiens Human MLSRKGIIPEEYVLTRLAEDPAEPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS kcnj11-g3i1t3_crigr-2 1858320 N/A 1858322 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1858323 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1B (Lane 1) kcnj11-g3i1t3_crigr-3 1858320 N/A 1858322 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1858323 unspecified role unspecified role prey prey N/A 1858324 inhibitor inhibitor unspecified role unspecified role region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1B (Lane 2) kcnj11-g3i1t3_crigr-1 1858321 N/A 1858322 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 1858323 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false Fig.s 1a (Lane 5), 2 (Lanes 3 & 4)