IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ srivastava-2014-4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pla proximity ligation assay pELISA pELISA PLA immunostaining immunostaining Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG., Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG. I choose the uniprot id of Ronquin 1 from antibody datasheet carola.vinuesa @anu.edu.au carola.vinuesa@anu.edu.au Nature communications 2014 imex curation Only protein-protein interactions srivastava-2014-1 human-t-cell Homo sapiens t-cell Peripheral blood T-lymphocytes rna-ip rna immunoprecipitation rna-ip qrt-pcr quantitative reverse transcription pcr QRT-PCR Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG., Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG. I choose the uniprot id of Ronquin 1 from antibody datasheet carola.vinuesa @anu.edu.au carola.vinuesa@anu.edu.au Nature communications 2014 imex curation Only protein-protein interactions srivastava-2014-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG., Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG. I choose the uniprot id of Ronquin 1 from antibody datasheet carola.vinuesa @anu.edu.au carola.vinuesa@anu.edu.au Nature communications 2014 imex curation Only protein-protein interactions srivastava-2014-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG., Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG. I choose the uniprot id of Ronquin 1 from antibody datasheet carola.vinuesa @anu.edu.au carola.vinuesa@anu.edu.au Nature communications 2014 imex curation Only protein-protein interactions srivastava-2014-5 mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG., Srivastava M., Duan G., Kershaw NJ., Athanasopoulos V., Yeo JH., Ose T., Hu D., Brown SH., Jergic S., Patel HR., Pratama A., Richards S., Verma A., Jones EY., Heissmeyer V., Preiss T., Dixon NE., Chong MM., Babon JJ., Vinuesa CG. I choose the uniprot id of Ronquin 1 from antibody datasheet carola.vinuesa @anu.edu.au carola.vinuesa@anu.edu.au Nature communications 2014 imex curation Only protein-protein interactions ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA rc3h1_human Roquin-1 RC3H1 KIAA2025 RNF198 RING finger and C3H zinc finger protein 1 RING finger protein 198 RING finger and CCCH-type zinc finger domain-containing protein 1 protein protein human Homo sapiens Human MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQPITLCSGVEDTKHYEEAKKCVEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAAILPDEGAVDLPSRKPPALPNGIVSTGNTVTQLIPRGTDPSYDSSLKPGKIDHLSSSAPGSPPDLLESVPKSISALPVNPHSIPPRGPADLPPMPVTKPLQMVPRGSQLYPAQQTDVYYQDPRGAAPPFEPAPYQQGMYYTPPPQCVSRFVRPPPSAPEPAPPYLDHYPPYLQERVVNSQYGTQPQQYPPIYPSHYDGRRVYPAPSYTREEIFRESPIPIEIPPAAVPSYVPESRERYQQIESYYPVAPHPTQIRPSYLREPPYSRLPPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPTFHPEEFLDEDLKVAGKYKGNDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGMRDQRLDLQRRAAETSDDDLIPFGDRPTVSRFGAISRTSKTIYQGAGPMQAMAPQGAPTKSINISDYSPYGTHGGWGASPYSPHQNIPSQGHFSERERISMSEVASHGKPLPSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREANTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP mint Represses ICOS function, an essential co-stimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1 ddx6_human Probable ATP-dependent RNA helicase DDX6 DDX6 HLR2 RCK DEAD box protein 6 ATP-dependent RNA helicase p54 Oncogene RCK protein protein human Homo sapiens Human MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP mir146a-1 microRNA 146A rna ribonucleic acid RNA rna human Homo sapiens Human TTGAGAACTGAATTCCATGGGT mmu-mir146a mmu-mir146a rna ribonucleic acid RNA rna mouse Mus musculus Mouse CUGAGAACUGAAUUCCAUGGGUUAUAUCAAUGUCA rc3h1_mouse Roquin-1 Rc3h1 Gm551 Kiaa2025 RING finger and C3H zinc finger protein 1 Protein Sanroque RING finger and CCCH-type zinc finger domain-containing protein 1 protein protein mouse Mus musculus Mouse MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNKLHRKACPFDQTTINTDIELLPVNSALLQLVGAQIPEQQPITLCSGVEDTKHYEEAKKCVEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLVDYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMNKRLVPRRPLSASLGQLNEVGLPSAPILSDESAVDLSNRKPPALPNGIASSGSTVTQLIPRGTDPSFDSSLKPVKLDHLSSSAPGSPPDLLESAPKSISALPVNPHPVPPRGPTDLPPMPVTKPIQMVPRGSQLYPAQQADVYYQDPRGSAPAFETAPYQQGMYYTPPPCVSRFVRPPPSAPEPGPPYLDHYSPYLQDRVINSQYGTQPQQYPPMYPAHYDGRRVYPAQSYTREEMFRESPIPIDIPSAAVPSYVPESRERYQQVEGYYPVAPHPAQIRPSYPRDPPYSRLPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPAFHPEEFLDEDLKVAGKYKANDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGTREQRLDLQRRAVETSDDDLIPFGDRPTVSRFGAISRTSKTLYQGAGPLQAIAPQGAPTKSINISDYSAYGAHGGWGDSPYSPHANIPPQGHFIEREKMSMAEVASHGKPLLSAEREQLRLELQQLNHQISQQTQLRGLEAVSNRLVLQREVNTLASQPQPPQLPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSVDMKSKLGTSKQAENGQPEPQNKIRTEDLTLTFSDVPNGSALTQENLSLLSNKTSSLNLSEDSEGGGDNNDSQRSGVVSNSAP ago2_mouse Protein argonaute-2 Ago2 Argonaute RISC catalytic component 2 Eif2c2 Eukaryotic translation initiation factor 2C 2 Kiaa4215 Piwi/argonaute family protein meIF2C2 Protein slicer protein protein mouse Mus musculus Mouse MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA roquin-ago2-2 1799628 N/A 1799633 unspecified role unspecified role neutral component neutral component N/A 1799634 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Fig7C ddx6-rc3h1-1 1799628 N/A 1799634 unspecified role unspecified role neutral component neutral component N/A 1799635 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Fig7C rc3h1-mir146a_1-1 1799629 N/A 1799634 unspecified role unspecified role bait bait N/A 1799636 unspecified role unspecified role prey prey physical association physical association false false false Fig5D roquin-mir146-2 1799630 N/A 1799637 unspecified role unspecified role bait bait biotin biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro Stoichiometry: 1.0 N/A 1799638 unspecified role unspecified role prey prey binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q4VGL6:p.Met199Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 direct interaction direct interaction false false false 1799630 Fig5EF Ronquin Mutation M199R maintains the binding ronquin-ago2-1 1799631 N/A 1799639 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 1799638 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false Fig7 ago2-rc3h1-2 1799632 N/A 1799639 unspecified role unspecified role bait bait N/A 1799638 unspecified role unspecified role prey prey physical association physical association false false false Suppl. Fig5B