IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ boutchueng_djidjou-2015-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Proteins from the plasma membrane and a Golgi/Endosome fraction were isolated after Insulin administration. robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation boutchueng_djidjou-2015-2 rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Proteins from the plasma membrane and a Golgi/Endosome fraction were isolated after Insulin administration. robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation boutchueng_djidjou-2015-1 rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes The Golgi/Endosome fraction containing Insulin receptor complexes was isolated after Insulin administration. Mass Spec identifications assigned to mouse proteins have been altered to equivalent rat proteins. Q6IN16 is a UniProt Secondary accession number; this had been reassigned to the Primary accession number O35567 (ATIC). robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation p06213-pro_0000016689 Insulin receptor subunit beta INSR protein protein human Homo sapiens Human SLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS 763 1382 pur9_human Bifunctional purine biosynthesis protein ATIC ATIC PURH OK/SW-cl.86 AICAR transformylase/inosine monophosphate cyclohydrolase protein protein human Homo sapiens Human MAPGQLALFSVSDKTGLVEFARNLTALGLNLVASGGTAKALRDAGLAVRDVSELTGFPEMLGGRVKTLHPAVHAGILARNIPEDNADMARLDFNLIRVVACNLYPFVKTVASPGVTVEEAVEQIDIGGVTLLRAAAKNHARVTVVCEPEDYVVVSTEMQSSESKDTSLETRRQLALKAFTHTAQYDEAISDYFRKQYSKGVSQMPLRYGMNPHQTPAQLYTLQPKLPITVLNGAPGFINLCDALNAWQLVKELKEALGIPAAASFKHVSPAGAAVGIPLSEDEAKVCMVYDLYKTLTPISAAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIIAPGYEEEALTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGVVDKSLFSNVVTKNKDLPESALRDLIVATIAVKYTQSNSVCYAKNGQVIGIGAGQQSRIHCTRLAGDKANYWWLRHHPQVLSMKFKTGVKRAEISNAIDQYVTGTIGEDEDLIKWKALFEEVPELLTEAEKKEWVEKLTEVSISSDAFFPFRDNVDRAKRSGVAYIAAPSGSAADKVVIEACDELGIILAHTNLRLFHH actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF kif5a_human Kinesin heavy chain isoform 5A Neuronal kinesin heavy chain Kinesin heavy chain neuron-specific 1 KIF5A NKHC1 protein protein human Homo sapiens Human MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS hacd3_human Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 Butyrate-induced protein 1 PTPLAD1 Protein-tyrosine phosphatase-like A domain-containing protein 1 HACD3 3-hydroxyacyl-CoA dehydratase 3 BIND1 protein protein human Homo sapiens Human MENQVLTPHVYWAQRHRELYLRVELSDVQNPAISITENVLHFKAQGHGAKGDNVYEFHLEFLDLVKPEPVYKLTQRQVNITVQKKVSQWWERLTKQEKRPLFLAPDFDRWLDESDAEMELRAKEEERLNKLRLESEGSPETLTNLRKGYLFMYNLVQFLGFSWIFVNLTVRFCILGKESFYDTFHTVADMMYFCQMLAVVETINAAIGVTTSPVLPSLIQLLGRNFILFIIFGTMEEMQNKAVVFFVFYLWSAIEIFRYSFYMLTCIDMDWKVLTWLRYTLWIPLYPLGCLAEAVSVIQSIPIFNETGRFSFTLPYPVKIKVRFSFFLQIYLIMIFLGLYINFRHLYKQRRRRYGQKKKKIH aapk2_human 5'-AMP-activated protein kinase catalytic subunit alpha-2 PRKAA2 AMPK AMPK2 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase protein protein human Homo sapiens Human MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR aapk2_rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase Prkaa2 Ampk Ampk2 protein protein rat Rattus norvegicus (Rat) Rat MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAQMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSIATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPTGSFMDDMAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSVDSSTAENHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR p15127-pro_0000016698 Insulin receptor subunit beta Insr protein protein rat Rattus norvegicus (Rat) Rat SLEEVGNVTATTPTLPDFPNISSTIAPTSHEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDSPEERSGVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRVRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPMGPLYASSNPEYLSASDVFPSSVYVPDEWEVPREKITLLRELGQGSFGMVYEGNAKDIIKGEVETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSHLRSLRPDAENNPGRPPPTLQEMIQMTAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTASSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDPPDNCPERLTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFYSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGCREGGSSLSIKRTYDEHIPYTHMNGGKKNGRVLTLPRSNPS 764 1383 pur9_rat Bifunctional purine biosynthesis protein ATIC AICAR transformylase/inosine monophosphate cyclohydrolase Atic Purh protein protein rat Rattus norvegicus (Rat) Rat MASSQLALFSVSDKTGLVEFARNLASLGLSLVASGGTAKAIRDAGLAVRDVSELTGFPEMLGGRVKTLHPAVHAGILARNIPEDAADMARLDFNLIRVVVCNLYPFVKTVASPDVTVEAAVEQIDIGGVTLLRAAAKNHARVTVVCEPEDYGAVAAEMQGSGNKDTSLETRRHLALKAFTHTAQYDEAISDYFRRQYSKGISQMPLRYGMNPHQTPAQLYTLKPKLPITVLNGAPGFINLCDALNAWQLVTELRGAVDIPAAASFKHVSPAGAAVGVPLSEDEARVCMVYDLYPTLTPLAIAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIVAPGYEEEALKILSKKKNGSYCVLQMDQSYKPDENEVRTLFGLRLSQKRNNGVVDKSLFSNIVTKNKDLPESALRDLIVATIAVKYTQSNSVCYAKDGQVIGIGAGQQSRIHCTRLAGDKANSWWLRHHPRVLSMKFKAGVKRAEVSNAIDQYVTGTIGEGEDLVKWKALFEEVPELLTEAEKKEWVDKLSGVSVSSDAFFPFRDNVDRAKRSGVAYIVAPSGSTADKVVIEACDELGIVLAHTDLRLFHH d4abi7_rat Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Hacd3 LOC100365676 RGD1565496_predicted rCG_58140 protein protein rat Rattus norvegicus (Rat) Rat METQVLTPHVYWAQRHRELYLRVELSDVQNPAISISENVLHFKAQGHGAKGDNVYEFHLEFLDLVKPEPAYRLTQRQVNITVQKKGSHWWERLTKQEKRPLFLAPDFDRWLDESDAEMELRAKEEERLNKLRLESEGSPETLTNLKKGYLFMYNLVQLLGFSWIFVNLTVRFFILGKESFYDTFHTVADMMYFCQMLALVETLNAAIGVTNSPVLPALIQFLGRNFILFLVFGTMEEMQNKAVVFFVFYSWSAIEIFRYPFYMLSCIDMDWKVLTWLRYTMWIPLYPLGCLSEAVAVIQSIPVFNESGRFSFTLPYPLRMKVRFSFFLQVYLVMLFLGLYINFRHLYKQRRRRYGQKKKKLH grb14_rat Growth factor receptor-bound protein 14 Grb14 GRB14 adapter protein protein protein rat Rattus norvegicus (Rat) Rat MTTSLQDGQSAAGRAGAQDSPLAVQVCRVAQGKGDAQDPAQVPGLHALSPASDATRRGAMDRRKAKDLEVQETPSIPNPFPELCCSPLTSVLSAGLFPRSNSRKKQVIKVYSEDETSRALEVPSDVTARDVCQLLILKNHYVDDNSWTLFEHLSHTGVERTVEDHELLTEVLSHWVMEEDNKLYLRKNYAKYEFFKNPMYFFPEHMVSFATEMNGDRSLTQIPQVFLSSNTYPEIHGFLHAKEQGKKSWKKAYFFLRRSGLYFSTKGTSKEPRHLQFFSEFSTSNVYMSLAGKKKHGAPTPYGFCFKPTKAGGPRDLKMLCAEEDQSRMCWVTAIRLLKYGMQLYQNYMHPSQARSACSSQSVSPMRSVSENSLVAMDFSGQKTRVIDNPTEALSVAVEEGLAWRKKGCLRLGNHGSPTAPSQSSAVNMALHRSQPWFHHRISRDEAQQLITRQGPVDGVFLVRDSQSNPRTFVLSMSHGQKIKHFQIIPVEDDGEVFHTLDDGHTKFTDLIQLVEFYQLNKGVLPCKLKHYCARMAV Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain mint plvap_rat Plasmalemma vesicle-associated protein Plasmalemma vesicle protein 1 gp68 Plvap Pv1 protein protein rat Rattus norvegicus (Rat) Rat MGLSMDRSPYSRTGDRDRGCWYYLRYFFLFVSLIQFLIILGLVLFMIYGNVHATTESSLRATEIRADNLYSQVVGLSAAQANLSKQLNISTLVKDTVMQQLLTTRREVERINASFRQCQGDLITYINYNRFIAAIILSEKQCQEQLKEGNKTCEALLFKLGEKVKTLEMEVVKEKAVCSKDKDSLLAGKRQAEMQQEACGKAREQQKQDQQVTEEQLRKVQSLCLPLDQEKFQADVLNVWRDSLVYRSLDNIGYHYSLMPEFSSLRRTCESLPGIMTTKVEELARGLRAGIERVTRENGELRRQKLELERAIQGEREARTRAGTEAQARETQLRTECARQTQLALEEKAALRTQRDDLERQLEARKRELEQLRTEVDVRISALDTCVKAKSLPAIQPRLPGPPPNPPPIDPASLEEFKKRILESQRPPLVNPAVPPSG copd_rat Coatomer subunit delta Archain Delta-coat protein Arcn1 Copd protein protein rat Rattus norvegicus (Rat) Rat MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLITTKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHEEKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKPKVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSNMGKRTSEAAKVHAPPINMESVHMKIEEKITLTCGRDGGLQNMELHGMIMLRISDDKFGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPEKSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAPVIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIPGQPNDFFPVQVSFISKKNYCNIQVTKVTQVDGNSPVRFSTETTFLVDKYEIL insr-actb-1 1796069 N/A 1796072 unspecified role unspecified role bait bait N/A 1796073 unspecified role unspecified role prey prey N/A 1796074 unspecified role unspecified role prey prey N/A 1796075 unspecified role unspecified role prey prey N/A 1796076 unspecified role unspecified role prey prey N/A 1796077 unspecified role unspecified role prey prey possible_phosphothronine opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false association association false false false 4 B+C Shown here are the results from the IP of the Insulin Receptor using scrambled siRNA. Insulin administration appeared to increase the phosphorylation of the IR as shown by use of a non-specific phosphotyrosine antibody. insr-kif5a-1 1796069 N/A 1796072 unspecified role unspecified role bait bait N/A 1796075 unspecified role unspecified role prey prey N/A 1796076 unspecified role unspecified role prey prey N/A 1796077 unspecified role unspecified role prey prey possible_phosphothreonine opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false association association false false false 4 B Shown here are the results from the IP of the Insulin Receptor using ATIC siRNA. ATIC depletion increased levels of phosphorylated AMPKA and KIF5A detected, but reduced levels of phosphorylated IR (in the presence of Insulin). insr-actb-3 1796069 N/A 1796072 unspecified role unspecified role bait bait N/A 1796073 unspecified role unspecified role prey prey N/A 1796074 unspecified role unspecified role prey prey N/A 1796076 unspecified role unspecified role prey prey association association false false false 4 C Shown here are the results from the IP of the Insulin Receptor using PTPLAD1 (HACD3) siRNA. Levels of this molecule were decreased, but not absent. Insulin administration appeared to increase the phosphorylation of the IR as shown by use of a non-specific phosphotyrosine antibody. insr-arcn1-2 1796070 N/A 1796078 unspecified role unspecified role prey prey N/A 1796079 unspecified role unspecified role bait bait N/A 1796080 unspecified role unspecified role prey prey N/A 1796081 unspecified role unspecified role prey prey association association false false false 2E, 3A IR is stated to be auto-phosphorylated as shown by the use of a non-specific anti-phospho-tyrosine antibody (PY20); however no molecular weight markers are shown to confirm the molecular weight (and presumed identity) of the band. In the Plasma Membrane fraction IR phosphorylation was greatest at ~30 seconds post Insulin injection, and then decreased. For the Golgi/Endosome fraction the peak was at 2 minutes post injection and then declined; however ATIC and a protein tyrosine phosphatase (PTP) inhibitor inhibited this decline. insr-atic-1 1796070 N/A 1796079 unspecified role unspecified role bait bait N/A 1796080 unspecified role unspecified role prey prey physical association physical association false false false 3B insr-arcn1-1 1796071 N/A 1796082 unspecified role unspecified role prey prey N/A 1796079 unspecified role unspecified role bait bait N/A 1796080 unspecified role unspecified role prey prey N/A 1796083 unspecified role unspecified role prey prey N/A 1796084 unspecified role unspecified role prey prey N/A 1796081 unspecified role unspecified role prey prey association association false false false Table 1