IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wu-2014-9 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip qrt-pcr quantitative reverse transcription pcr QRT-PCR Wu H., Becker D., Krebber H. heike.krebber@biologie.uni-goettingen.de Cell reports 2014 imex curation Only protein-protein interactions wu-2014-10 yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wu H., Becker D., Krebber H. heike.krebber@biologie.uni-goettingen.de Cell reports 2014 imex curation Only protein-protein interactions tlc1_yeast_rna Yeast integral telomerase RNA template component, TLC1 rna ribonucleic acid RNA rna yeast Saccharomyces cerevisiae Baker's yeast AAUAAAACUAGAGAGGAAGAUAGGUACCCUAUGAAAAUGUCAAUGGCUGUUGCGUUUGCUUAAUCGUAUUUUUUUUUUUUUCAGUCCGUGUUUUUUGUACAUUCUACGUUUGAGUUUUCCAUCAUGCAGGCCUCAGAAAUUUGGUAGGCACUCGAUGGUGAAGAGAUAGUGUCGGAUUUCGGAUUGAUCUUUCAGUUGAUAGCCUGCUGCUCUUUUCUUUUCCAAAGAAUUUCGAGUAUGCUGGUGUCAGUGUAGAUGCUUGUGUGUGCGCAAUUUGUGGUUUUUUAUUGUGUUUCUACUUAUAGAUGGCUAAAAUCUGAGUUUAGAAAAUGCAAACCGUAAAUUCUUAAACACUGCUAUUGCAUUUAGUUGCUAAAGCAGUGUUUUUGAACUUAUUCCUGUUAUUCCUUCUUCGUACCGAUCCUCUUCUCGACCUAACCUUUUAAUUACCAUGGGAAGCCUACCAUCACCACACCCACACACAAAUGUUACAGCUAAUUGUUUAUUAGCAAAGUUUGCACGAGUUCGCUGUUUAUUUUUUUCUCGUUUUCUUAUACCUAGUAUUUUUUCUGACACUGUUUAAGGUGACAGAAAAAAAGGAGUUUAAGUUAGAUUUGCAAACAGACGGUGCUAAGCGCUGUCACUUUAUGUCUAUCUUAUCGUUAACUCUGGAAAAAGAAAAAGGAAAAAGAACGUCAGGGAACAUGAGUAUAUAUAGAAAUGGUUUAUUCUAGUUUUUUCCGUUUUUUCAGUAGAUUUUUGCCUUUAAAAGAAUAAAUCCCACUACAAAAAGGUAAAAUAAAAAAUCUAUUCACUGAACUUACUGAUGAAAUUUCCAAAUGUGCCCCGUACAUCGAACGAUGUGACAGAGAAAAAUACGAGUAGGUAAAUAAGCCAAAAGGCAAGGGUGUCCUUUCUUAAGCAUCGGUUAGGUUUGCGGGCGAUCAGUAACUGAACAAUGACACAAGAUCAAGAACGUAAUUUGAGAUUUUUCAAGAUGGUUUUUUUAGGUAUCUAUUAAAACUACUUUGAUGAUCAAUACGGUAUUUUUGUCGCAUUAUUUUCCAAGCGGAAGGAACCGUGUGUUCAUUUUAUGAAUCUUGGUGUUGUAUUCACAGCUACUUCUCCUAAUGCCUUCGAUGCAUUUAGAUAAUUUUUGGAAACAUUUUUUUUCUUGAUGUAUAUUUUUUGUAUUGUAGAAAUCGCGCGUACUGUACUUGUAUAUCGCUUUAUAAGCGCUUUUAAUUGAUUGUUCAUGACGAGGAUAGGCGGAUAGGCGGAGGUAUGCCUCUUAAUAUUUA TLC1 RNA contains a template sequence that Est2p uses to add irregular repeats of TG(1-3) residues onto a DNA end; RNA template component of telomerase xpo1_yeast Exportin-1 CRM1 KAP124 YGR218W G8514 XPO1 Chromosome region maintenance protein 1 Karyopherin-124 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDNPDAWQKADQILQFSTNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQKNLINKSDLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAEQMTQAKALHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLLRYLHWIPYRYIYETNILELLSTKFMTSPDTRAITLKCLTEVSNLKIPQDNDLIKRQTVLFFQNTLQQIATSVMPVTADLKATYANANGNDQSFLQDLAMFLTTYLARNRALLESDESLRELLLNAHQYLIQLSKIEERELFKTTLDYWHNLVADLFYEVQRLPATEMSPLIQLSVGSQAISTGSGALNPEYMKRFPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGEIVREFVKESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTLSWAIGSISGTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFLKAHWNFLRTVILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQTIIRDIQKTTADLQPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQSTANPTLLLDSETVKIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMISAQVAAEGLIATKTPKVRGLRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLEDYMNNVPDARDAEVLNCMTTVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHRVEFYKLLKVINEKSFAAFLELPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKNIERMGNVPFANEFHKNYFFIFVSETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKISVPLYQEAEVPQGTSNQVYLSQYLANMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKGTLRDFLVQIKEVGGDPTDYLFAEDKENALMEQNRLEREKAAKIGGLLKPSELDD A81833C7F60F5202 tert_yeast Telomerase reverse transcriptase EST2 Telomerase catalytic subunit L8543.12 YLR318W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKILFEFIQDKLDIDLQTNSTYKENLKCGHFNGLDEILTTCFALPNSRKIALPCLPGDLSHKAVIDHCIIYLLTGELYNNVLTFGYKIARNEDVNNSLFCHSANVNVTLLKGAAWKMFHSLVGTYAFVDLLINYTVIQFNGQFFTQIVGNRCNEPHLPPKWAQRSSSSSATAAQIKQLTEPVTNKQFLHKLNINSSSFFPYSKILPSSSSIKKLTDLREAIFPTNLVKIPQRLKVRINLTLQKLLKRHKRLNYVSILNSICPPLEGTVLDLSHLSRQSPKERVLKFIIVILQKLLPQEMFGSKKNKGKIIKNLNLLLSLPLNGYLPFDSLLKKLRLKDFRWLFISDIWFTKHNFENLNQLAICFISWLFRQLIPKIIQTFFYCTEISSTVTIVYFRHDTWNKLITPFIVEYFKTYLVENNVCRNHNSYTLSNFNHSKMRIIPKKSNNEFRIIAIPCRGADEEEFTIYKENHKNAIQPTQKILEYLRNKRPTSFTKIYSPTQIADRIKEFKQRLLKKFNNVLPELYFMKFDVKSCYDSIPRMECMRILKDALKNENGFFVRSQYFFNTNTGVLKLFNVVNASRVPKPYELYIDNVRTVHLSNQDVINVVEMEIFKTALWVEDKCYIREDGLFQGSSLSAPIVDLVYDDLLEFYSEFKASPSQDTLILKLADDFLIISTDQQQVINIKKLAMGGFQKYNAKANRDKILAVSSQSDDDTVIQFCAMHIFVKELEVWKHSSTMNNFHIRSKSSKGIFRSLIALFNTRISYKTIDTNLNSTNTVLMQIDHVVKNISECYKSAFKDLSINVTQNMQFHSFLQRIIEMTVSGCPITKCDPLIEYEVRFTILNGFLESLSSNTSKFKDNIILLRKEIQHLQAYIYIYIHIVN 84540735E3CD40F5 est1_yeast Telomere elongation protein EST1 EST1 YLR233C L8083.15 Ever shorter telomeres protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNEEVNEECMRLFFKNARAHLDKHLTSRLTCDENAYITFRCFLDGIHRKSTRFLEELLLKQENMYHNNNYERINDSVIPLVLKLLWLQIHEPTLQWFEHWFHDIMRLSNRRKFRVFRIFQKKMIQFFKITHRYYYDIIEHLCAKYDMNSVISNALFAKLNLMQYTDGLSTHEKIILNTSNPLTFSIVISLQRCVINLGSTHFYKTLLNKPSNKPKSVEGFEKSIRYLNIASLYLPAVGDTYFQRAKIYLITGKFSLYFFELVRGALVRIPSKCALNNLKDFILTPDFPERRRLMKKLAILVSKDLKGEKSFFEGQIVLQFLSIVEHTLVPQSWNASRASNCWLLKEHLQMAALKYHSGNINVILENLAATMGSFDLMFTTRKSKEQKNKLKYADLSERQVFFLDLSFDFIANIIDVVIKPSWQKNMEDFRYLAIIRLLMCWIKSYRSILQYTHRHRKFCTSFALLLNDLINSPLNCSGNIYSHRPKRSYLFREDIIFREFSCINFALTDFNDDYVYDSPDMINNIIGCPTLTKVLSPKEECVLRIRSIIFSGMKFLEKNDTGVIWNASKYKFDLISPNIKIKRQIALSEISSKINVKTQQERVVSSRKVEAKRDEQQRKRAGKIAVTELEKQFANVRRTKKLSPLPEKDGVSSELVKHAASRGRKTITGPLSSDFLSYPDEAIDADEDITVQVPDTPT 9CF552AFDA6BDEDE mex67_yeast mRNA export factor MEX67 MEX67 P2520 YPL169C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGFHNVGNINMMAQQQMQQNRIKISVRNWQNATMNDLINFISRNARVAVYDAHVEGPLVIGYVNSKAEAESLMKWNGVRFAGSNLKFELLDNNGASAGTSDTISFLRGVLLKRYDPQTKLLNLGALHSDPELIQKGVFSSISTQSKMFPAMMKLASTEKSLIVESVNLADNQLKDISAISTLAQTFPNLKNLCLANNQIFRFRSLEVWKNKFKDLRELLMTNNPITTDKLYRTEMLRLFPKLVVLDNVIVRDEQKLQTVYSLPMKIQQFFFENDALGQSSTDFATNFLNLWDNNREQLLNLYSPQSQFSVSVDSTIPPSTVTDSDQTPAFGYYMSSSRNISKVSSEKSIQQRLSIGQESINSIFKTLPKTKHHLQEQPNEYSMETISYPQINGFVITLHGFFEETGKPELESNKKTGKNNYQKNRRYNHGYNSTSNNKLSKKSFDRTWVIVPMNNSVIIASDLLTVRAYSTGAWKTASIAIAQPPQQQASVLPQVASMNPNITTPPQPQPSVVPGGMSIPGAPQGAMVMAPTLQLPPDVQSRLNPVQLELLNKLHLETKLNAEYTFMLAEQSNWNYEVAIKGFQSSMNGIPREAFVQF 6CE55EEC6FA40B40 dbp5_yeast ATP-dependent RNA helicase DBP5 YOR046C DBP5 RAT8 DEAD box protein 5 Helicase CA5/6 Ribonucleic acid-trafficking protein 8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDTKRDPADLLASLKIDNEKEDTSEVSTKETVKSQPEKTADSIKPAEKLVPKVEEKKTKQEDSNLISSEYEVKVKLADIQADPNSPLYSAKSFDELGLAPELLKGIYAMKFQKPSKIQERALPLLLHNPPRNMIAQSQSGTGKTAAFSLTMLTRVNPEDASPQAICLAPSRELARQTLEVVQEMGKFTKITSQLIVPDSFEKNKQINAQVIVGTPGTVLDLMRRKLMQLQKIKIFVLDEADNMLDQQGLGDQCIRVKRFLPKDTQLVLFSATFADAVRQYAKKIVPNANTLELQTNEVNVDAIKQLYMDCKNEADKFDVLTELYGLMTIGSSIIFVATKKTANVLYGKLKSEGHEVSILHGDLQTQERDRLIDDFREGRSKVLITTNVLARGIDIPTVSMVVNYDLPTLANGQADPATYIHRIGRTGRFGRKGVAISFVHDKNSFNILSAIQKYFGDIEMTRVPTDDWDEVEKIVKKVLKD C50CAFE8C060D46B ku70_yeast ATP-dependent DNA helicase II subunit 1 YKU70 HDF1 NES24 YMR284W YM8021.10 ATP-dependent DNA helicase II subunit Ku70 High affinity DNA-binding factor subunit 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MRSVTNAFGNSGELNDQVDETGYRKFDIHEGILFCIELSETMFKESSDLEYKSPLLEILESLDELMSQLVITRPGTAIGCYFYYCNREDAKEGIYELFPLRDINATFMKKLNDLLEDLSSGRISLYDYFMFQQTGSEKQVRLSVLFTFMLDTFLEEIPGQKQLSNKRVFLFTDIDKPQEAQDIDERARLRRLTIDLFDNKVNFATFFIGYADKPFDNEFYSDILQLGSHTNENTGLDSEFDGPSTKPIDAKYIKSRILRKKEVKRIMFQCPLILDEKTNFIVGVKGYTMYTHEKAGVRYKLVYEHEDIRQEAYSKRKFLNPITGEDVTGKTVKVYPYGDLDINLSDSQDQIVMEAYTQKDAFLKIIGFRSSSKSIHYFNNIDKSSFIVPDEAKYEGSIRTLASLLKILRKKDKIAILWGKLKSNSHPSLYTLSPSSVKDYNEGFYLYRVPFLDEIRKFPSLLSYDDGSEHKLDYDNMKKVTQSIMGYFNLRDGYNPSDFKNPLLQKHYKVLHDYLLQIETTFDENETPNTKKDRMMREDDSLRKLYYIRNKILESEKSEDPIIQRLNKYVKIWNMFYKKFNDDNISIKEEKKPFDKKPKFNI BD58160328EE6C6E xpo_tlc1-1 1883288 N/A 1883290 unspecified role unspecified role prey prey N/A 1883291 unspecified role unspecified role bait bait tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig1 D, Fig2D est1_tlc1-1 1883288 N/A 1883290 unspecified role unspecified role prey prey N/A 1883292 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1883293 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false Fig1D mex67_tlc1-1 1883288 N/A 1883290 unspecified role unspecified role prey prey N/A 1883294 unspecified role unspecified role bait bait tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q99257:p.His400Tyr mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig1D, Fig2D rat8_tlc1-1 1883288 N/A 1883290 unspecified role unspecified role prey prey N/A 1883295 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig1D est2_tlc1-1 1883288 N/A 1883290 unspecified role unspecified role prey prey N/A 1883292 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig3D mutations in Xpo1, Rat7, Rat8 and mex67 diminish the binding est1_est2-1 1883289 N/A 1883292 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1883293 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig3 E The deletion of Tlc1 diminishes the binding est2_yku70-1 1883289 N/A 1883292 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1883296 unspecified role unspecified role prey prey physical association physical association false false false Fig3G