IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gould-2014-2 Saccharomyces cerevisiae Kelch proteins and Bud14 protein form a stable 520-kDa formin regulatory complex that controls actin cable assembly and cell morphogenesis. in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Gould C.J., Chesarone-Cataldo M., Alioto S.L., Salin B., Sagot I., Goode B.L. Protein was first fractionated on sucrose gradients and then purified on Superose 6 gel filtration columns goode@brandeis.edu J. Biol. Chem. (0021-9258) 2014 Only protein-protein interactions imex curation gould-2014-1 Saccharomyces cerevisiae Kelch proteins and Bud14 protein form a stable 520-kDa formin regulatory complex that controls actin cable assembly and cell morphogenesis. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Gould C.J., Chesarone-Cataldo M., Alioto S.L., Salin B., Sagot I., Goode B.L. Protein was first fractionated on sucrose gradients and then purified on Superose 6 gel filtration columns goode@brandeis.edu J. Biol. Chem. (0021-9258) 2014 Only protein-protein interactions imex curation kel1_yeast Kelch repeat-containing protein 1 KEL1 YHR158C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGFSFAKKFTHKKHGKTPSDASISDQSREASLSTPPNEKFFTKQETPQKGRQFSQGYHSNVNKTSSPPMFARKQVSESRIQPSAVPPQQRNVSGPSTTLHKQLSKQREYTVWNRIKLQNSPFPRYRHVASAYVTDKNQIYVIGGLHDQSVYGDTWILTAFDNATRFSTTTIDISEATPPPRVGHAAVLCGNAFVVFGGDTHKVNKEGLMDDDIYLLNINSYKWTVPAPVGPRPLGRYGHKISIIATTQMKTKLYVFGGQFDDTYFNDLAVYDLSSFRRPDSHWEFLKPRTFTPPPITNFTMISYDSKLWVFGGDTLQGLVNDVFMYDPAINDWFIIDTTGEKPPPVQEHATVVYNDLMCVVGGKDEHDAYLNSVYFLNLKSRKWFKLPVFTAGIPQGRSGHSLTLLKNDKILIMGGDKFDYARVEEYDLHTSDIDMQRGTIVYTLDLARIKDLCPGVMDVPTDTPTPRNGNLDLATPVTPTSHQTKNMNVPISAAPLASAPSPAPKDFSDADRLNREVHNRNVSTEHQNQSHPVNSESHLIAEPNILTPYVPSESSQTPVMKITSNKPFDTPTIQKEPDLSETMDPTVGNQRIPSSIYGDNLTPANQIKNNSPILETLPSNEIKTPQNGNIEEIKHLPDADEKIDSTTTFDQEINGDKLGTSSMSKVEEDGNVADEDDEIGVAQMASSPSKDQFKIKHYNESSELSQNNTEIDKLSEPVDITIKKSDTAGHDSANHVIDASDEKNVSPMGDVPTDTKNEEASVPINRDATTEVVDRALFEKLRSELQSLKELTHEKALEAGAHIKELETELWQLKSQKNSGTTKEIDELDSVRLQSKCEILEADNHSLEDKVNELEELVNSKFLDIENLNEVIQFQNEKIKSLELEPNYKEKLEELQIEHENLSRENERLKNESKQHNEDIINNVANYSSQLGSLISHWKENRANSSFLESSSSLISVSDENGEKTVGEPYGDQSRHHRVVINKLTNRLDDLLERSQELTISKEKLSSEYHALKMEHSSLSQDVLVKENEIKKIQNDYKESISSMDSASKALMVSQRELEKYKSLNKKLIDELDELKFKNGVCSENFENGLRSTEESSNNVKNSNSIRENQFNIKINDLKAELFITNQERDDLKSEVLELKKRLLNLENNTKQVNEDADSDLL 43D0FC570F1D5E4D bud14_yeast Bud site selection protein 14 FUN2 YAR014C BUD14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKEEHVDETSASGVKEVSSIAARHDNGYAPSLITSTSGMDSFQSHALLNDPTLIEDYSDIINNRPTSGSKLTLGNEDSESMGGSVVVTPTSNKSSPFNSKLNILSNAAEKGHDVLRNRDDDKELEEENVEKHMHSNSKRDQRHYKENSSELPDSYDYSDSEFEDNLERRLQEIETDSVDSADKDEVHFSVNNTMNPDVDDFSDGLKYAISEDEDEEENYSDDDDFDRKFQDSGFQGEKDDLEEENDDYQPLSPPRELDPDKLYALYAFNGHDSSHCQLGQDEPCILLNDQDAYWWLVKRITDGKIGFAPAEILETFPERLARLNCWKNENMSSQSVASSDSKDDSISSGNKNQSDAESIIPTPALNGYGKGNKSVSFNDVVGYADRFIDDAIEDTSLDSNDDGGEGNGQSYDDDVDNDKETKVTHRDEYTEAKLNFGKFQDDDTSDVVSDVSFSTSLNTPLNVKKVRRQDNKNESEPKTSSSKDREDDYNANRYVGQEKSEPVDSDYDTDLKKVFEAPRMPFANGMAKSDSQNSLSTIGEFSPSSSEWTNESPSTPIVEESSSIPSSRAIKDISQYIHAKSKIEETTNVENTEGQIQASLGSSGGMANQTDAEQPKEELEKHHSTPEEEKQSTLSLHSSSEEDFYMDEQRAVSSASINSSLSGSRALSNTNMSDPASKPNSLVQHLYAPVFDRMDVLMKQLDEIIRK F123511A4C592EB4 kel2_yeast Kelch repeat-containing protein 2 KEL2 G8585 YGR238C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVPFKLTNKVPTDTGPSLISAQSVPRPIVFMDNRNNTRIVTPTLPPNQHRGISGASTALPWSPESKNTGKYIWNRVKLKNSPFPRYRHSSSFIVTNDNRIFVTGGLHDQSVYGDVWQIAANADGTSFTSKRIDIDQNTPPPRVGHASTICGNAYVVFGGDTHKLNKNGLLDDDLYLFNINSYKWTIPQPIGRRPLGRYGHKISIIASNPMQTKLYLFGGQVDETYFNDLVVFDLSSFRRPNSHWEFLEPVGDLPPPLTNHTMVAYDNKLWVFGGETPKTISNDTYRYDPAQSEWSKVKTTGEKPPPIQEHASVVYKHLMCVLGGKDTHNAYSNDVYFLNLLSLKWYKLPRMKEGIPQERSGHSLTLMKNEKLLIMGGDKTDYASPNIHDLQTSETDQGEGTLLYTLDLSSLNELCPGIMCESLHAGESFSNSLSGGFTPSKSTESENQEIINILTPRLPDSKVLSYNDIDEGAGSYSSALDDKAFERKSDREEKKPQSSKVDSSINKESPGTGIKVSKKNFPVLRGLTVDSEEYGSSSYKDTSCQKGIPKNLFDDLNLNLQTLRLEAQQKELETARHISQLEKEVQRLMVIKEASKDSNFQTARLKNLEIQKTFLESRINDLKNLLMVKLSQASKLCDQITIQNNGLKTCSEHVTIKRDIIDLENKCDVLKRQNEILVNNMQKITPELHTYLNESSCYLGKLLKSYPTSARPPSSEKDNQIYEKDSLNKIEKVINEMHETVRAKEKLHLETQKLNDERDSLRANLLDNNNKLDALRKLSDGSSKSMDLTKKAIHLSQSELEKYRKNNDDLQKEIDRIKTEQAEQDDKQEQRGAITHGNFDAFHRMKINNLKAELYMSKENRDSLKDELLALKKKLYTLEQKK 63B9772FF017E6D9 bud14-kel1-1 1775808 n/a 1775810 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1775811 unspecified role unspecified role neutral component neutral component n-terminal mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig. 2B bud14-kel1-2 1775808 n/a 1775812 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1775811 unspecified role unspecified role neutral component neutral component n-terminal mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig. 2C bud14-1 1775809 n/a 1775811 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 1 Mass of complex was approx 140kDa