MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint fukutomi-2014-1 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Fukutomi T., Takagi K., Mizushima T., Ohuchi N., Yamamoto M. masiyamamoto@med.tohoku.ac.jp Molecular and cellular biology 2014 Only protein-protein interactions imex curation imex curation fukutomi-2014-2 in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Fukutomi T., Takagi K., Mizushima T., Ohuchi N., Yamamoto M. masiyamamoto@med.tohoku.ac.jp Molecular and cellular biology 2014 Only protein-protein interactions imex curation imex curation fukutomi-2014-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Fukutomi T., Takagi K., Mizushima T., Ohuchi N., Yamamoto M. masiyamamoto@med.tohoku.ac.jp Molecular and cellular biology 2014 Only protein-protein interactions imex curation imex curation fukutomi-2014-5 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fukutomi T., Takagi K., Mizushima T., Ohuchi N., Yamamoto M. masiyamamoto@med.tohoku.ac.jp Molecular and cellular biology 2014 Only protein-protein interactions imex curation imex curation fukutomi-2014-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Fukutomi T., Takagi K., Mizushima T., Ohuchi N., Yamamoto M. masiyamamoto@med.tohoku.ac.jp Molecular and cellular biology 2014 Only protein-protein interactions imex curation imex curation keap1_mouse Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 Keap1 Inrf2 Kiaa0132 protein protein mouse Mus musculus Mouse MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTPSQDGNRTFSYTLEDHTKQAFGVMNELRLSQQLCDVTLQVKYEDIPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIEFAYTASISVGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYIYMHFGEVAKQEEFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHALTPRFLQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQSLSYLEAYNPSNGSWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCASMSVPRNRIGVGVIDGHIYAVGGSHGCIHHSSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITPMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRHHRSALGITVHQGKIYVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQNCTC nf2l2_mouse Nuclear factor erythroid 2-related factor 2 Nuclear factor, erythroid derived 2, like 2 Nfe2l2 Nrf2 protein protein mouse Mus musculus Mouse MMDLELPPPGLQSQQDMDLIDILWRQDIDLGVSREVFDFSQRQKDYELEKQKKLEKERQEQLQKEQEKAFFAQFQLDEETGEFLPIQPAQHIQTDTSGSASYSQVAHIPKQDALYFEDCMQLLAETFPFVDDHESLALDIPSHAESSVFTAPHQAQSLNSSLEAAMTDLSSIEQDMEQVWQELFSIPELQCLNTENKQLADTTAVPSPEATLTEMDSNYHFYSSISSLEKEVGNCGPHFLHGFEDSFSSILSTDDASQLTSLDSNPTLNTDFGDEFYSAFIAEPSDGGSMPSSAAISQSLSELLDGTIEGCDLSLCKAFNPKHAEGTMEFNDSDSGISLNTSPSRASPEHSVESSIYGDPPPGFSDSEMEELDSAPGSVKQNGPKAQPAHSPGDTVQPLSPAQGHSAPMRESQCENTTKKEVPVSPGHQKAPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVDDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLGHLKDEREKLLREKGENDRNLHLLKRRLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDTKKN 1-7 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Trp24Cys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-1 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Trp24Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-2 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Gln26Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-3 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Asp27Tyr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-4 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Ile28Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-5 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Asp29Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-6 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Asp29His mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-7 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Leu30Phe mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-8 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Gly31Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-9 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Val32Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-10 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Arg34Gln mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-11 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Leu23Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-12 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Ser33Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-13 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q60795:p.Gln43Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f2b f2c nfe2l2-keap1-14 1791275 N/A 1791280 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1791281 unspecified role unspecified role bait bait purified purified range sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gst-his tag tandem tag tandem tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f3 the combined deletion analysis revealed that the region spanning Met17 to Tyr46 is essential and sufficient for Keap1 binding (Fig. 3B). We refer to this region as Nrf2-DLG. The results also indicate that the region corresponding to Met17 to Gln51 retains slightly stronger Keap1-binding activity. Therefore, we designated Met17 to Gln51 as the extended DLG motif of Nrf2 (Nrf2-DLGex) in this study. 1-8 1791276 N/A 1791281 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f4 keap1-nfe2l2-16 1791276 N/A 1791281 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f4 keap1-nfe2l2-17 1791277 N/A 1791281 peptide synthesis peptide synthesis acceptor acceptor neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 acceptor acceptor neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f5 t1 ka (106 M-1) 38 keap1-nfe2l2-18 1791277 N/A 1791281 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f5 t1 ka (106 M-1) 1.9 keap1-nfe2l2-20 1791278 N/A 1791281 peptide synthesis peptide synthesis unspecified role unspecified role unspecified role unspecified role purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role unspecified role unspecified role purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f8 t3 keap1-nfe2l2-21 1791278 N/A 1791281 peptide synthesis peptide synthesis unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f8 t3 keap1-nfe2l2-19 1791279 N/A 1791281 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 1791280 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false t2 f6 f7 we crystallized Keap1-DC in complex with a long DLG peptide, DLGex(Met17-Gln51), and analyzed the crystal by X-ray diffraction at a 1.6 Å resolution (Table 2). The asymmetric unit contains two heterodimers of Keap1-DC-DLGex (chains A to B and L to M).