HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html chi-2012-4 in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn PloS one 2012 Only protein-protein interactions imex curation imex curation chi-2012-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn PloS one 2012 Only protein-protein interactions imex curation imex curation chi-2012-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn PloS one 2012 Only protein-protein interactions imex curation imex curation chi-2012-1 chick-embryonic_fibroblast Chick embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M., Chi XJ., Lu YX., Zhao P., Li CG., Wang XJ., Wang M. wangxj@cau.edu.cn PloS one 2012 Only protein-protein interactions imex curation imex curation gh_gahvr Envelope glycoprotein H protein protein Gallid herpesvirus 2 Gallid herpesvirus 2 SPPGHGAHVRENVELOPEGLYCOPROTEINHOSGALLIDHERPESVIRUSSTRAINRBBGNGHPESVMGLPGSIVFLIMIHAFCAKKTPTNTLPSLLSLLGITDLPSLRLNILSLDGSANNQGSWVRDNTTFVYIGASSPANGVLFYMPTSHVQQMTFYKRPVSKLLASNNLIKFLNTGSYINHSFMTAMPPYRRNVQIPSDRSGLKLDDKDDAQPTGTNPPTELKNLKPIDVVNPEHRFILTSELTGTYVKHVCFVDPMDMLIPVDYAHIRTIIFGSDGAEVIMKIGITFASITISMKSAPPVELILSERARNISLIWPALKPYEPVDKFTRRPYLIYLLGPHMNASDMEIKSYINMIESVEESSNYDFQIAQTHAQLFIFAATPISDINDIYCFRVVTTRLFMSLVASVRNAFQSGYISFDEIIKTEANIKMITETLSTFALHSNPGTYFLLSGMHLRNENADIIKSLIRKTIINASKNTASLSILQHLYVLRSAYAFNISQESGNLGEHVSSISLELIIALHEESVRDTIAWNTSARHALYYAFASIFQRPPNEWDASRTARKALLFASSMCTEEHIVATELVIQEMYIKINVKNSPVHILDVYTPCVTALRMDISEHHHRLYAMSDVILHPVIEKYLENDSRGIDAEEELETKAELVITKLKTPLMRRLTIYASEVVTCSDADILEATALLVLPISGLGSYVVTRQLGIRGIVYNVDGVDVNNQLYITYVRLPCTTTAGNIVPMVLPRPLGSDCPYCGCVLLRYSTNGNLRHTIYISSQDLQRELIAGGNSSIRYFNPTIAQIYGTSLLLYPNGTIVRILAFESERVTIISATYVATATAGASIAISIAIITVRMRINNFRYNYHRYKKLSLYDDL gb_gahvr Envelope glycoprotein B protein protein Gallid herpesvirus 2 Gallid herpesvirus 2 SPPGBGAHVRENVELOPEGLYCOPROTEINBOSGALLIDHERPESVIRUSSTRAINRBBGNGBPESVMHYFRRNCIFFLIVILYGTNSSPSTQNVTSREVVSSVQLSEEESTFYLCPPPVGSTVIRLEPPRKCPEPRKATEWGEGIAILFKENISPYKFKVTLYYKNIIQTTTWTGTTYRQITNRYTDRTPVSIEEITDLIDGKGRCSSKARYLRNNVYVEAFDRDAGEKQVLLKPSKFNTPESRAWHTTNETYTVWGSPWIYRTGTSVNCIVEEMDARSVFPYSYFAMANGDIANISPFYGLSPPEAAAEPMGYPQDNFKQLDSYFSMDLDKRRKASLPVKRNFLITSHFTVGWDWAPKTTRVCSMTKWKEVTEMLRATVNGRYRFMARELSATFISNTTEFDPNRIILGQCIKREAEAAIEQIFRTKYNDSHVKVGHVQYFLALGGFIVAYQPVLSKSLAHMYLRELMRDNRTDEMLDLVNNKHAIYKKNATSLSRLRRDIRNAPNRKITLDDTTAIKSTSSVQFAMLQFLYDHIQTHINDMFSRIATAWCELQNRELVLWHEGIKINPSATASATLGRRVAAKMLGDVAAVSSCTAIDAESVTLQNSMRVITSTNTCYSRPLVLFSYGENQGNIQGQLGENNELLPTLEAVEPCSANHRRYFLFGSGYALFENYNFVKMVDAADIQIASTFVELNLTLLEDREILPLSVYTKEELRDVGVLDYAEVARRNQLHELKFYDINKVIEVDTNYAFMNGLAELFNGMGQVGQAIGKVVVGAAGAIVSTISGVSAFMSNPFGALAIGLIIIAGLVAAFLAYRYVNKLKSNPMKALYPMTTEVLKAQATRELHGEESDDLERTSIDERKLEEAREMIKYMALVSAEERHEKKLRRKRRGTTAVLSDHLAKMRIKNSNPKYDKLPTTYSDSEDDAV gh_gahvr-gh_gahvr-1 1852410 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 5 and 6 gb_gahvr-gh_gahvr-4 1852410 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false direct interaction direct interaction false false false 5 and 6 gb_gahvr-gh_gahvr-5 1852410 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false direct interaction direct interaction false false false 5 and 6 gb_gahvr-gh_gahvr-2 1852410 N/A 1852414 competitor competitor competitor unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852414 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false 5 gh_gahvr-2 1852410 N/A 1852414 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 self interaction self interaction false false false 5 gb_gahvr-6 1852410 N/A 1852415 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false Stoichiometry: 3.0 self interaction self interaction false false false 5 gh_gahvr-3 1852410 N/A 1852414 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 self interaction self interaction false false false 5 gb-gh-3 1852411 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false direct interaction direct interaction false false false 4 and table 1 gh-gh-2 1852411 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 4 and table 1 gb-gh-4 1852411 N/A 1852414 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false direct interaction direct interaction false false false 4 and table 1 gh_gahvr-1 1852411 N/A 1852414 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 self interaction self interaction false false false 4 and table 1 gb_gahvr-1 1852411 N/A 1852415 unspecified role unspecified role putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false Stoichiometry: 3.0 self interaction self interaction false false false 4 and table 1 gb_gahvr-gh_gahvr-3 1852411 N/A 1852414 competitor competitor competitor unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852414 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false association association false false false 4 and table 1 gb-gh-2 1852412 N/A 1852414 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3 gh-gh-1 1852412 N/A 1852414 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1852414 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3 gb-gh-5 1852412 N/A 1852414 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1852415 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3 gb-gh-1 1852413 N/A 1852415 unspecified role unspecified role prey prey N/A 1852414 unspecified role unspecified role bait bait physical association physical association false false false 2 gb_gahvr-gh_gahvr-1 1852413 N/A 1852415 unspecified role unspecified role bait bait N/A 1852414 unspecified role unspecified role prey prey physical association physical association false false false 2