UniProt UniProt http://www.uniprot.org couarch-2011-1 Lafora progressive myoclonus epilepsy: NHLRC1 mutations affect glycogen metabolism. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining Couarch P., Vernia S., Gourfinkel-An I., Lesca G., Gataullina S., Fedirko E., Trouillard O., Depienne C., Dulac O., Steschenko D., Leguern E., Sanz P., Baulac S. Origin of laforin/ EPM2A according pubmed 14532330 (following cited reference 10 in publication) stephanie.baulac@upmc.fr J. Mol. Med. (0946-2716) 2011 imex curation Only protein-protein interactions couarch-2011-2 Lafora progressive myoclonus epilepsy: NHLRC1 mutations affect glycogen metabolism. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Couarch P., Vernia S., Gourfinkel-An I., Lesca G., Gataullina S., Fedirko E., Trouillard O., Depienne C., Dulac O., Steschenko D., Leguern E., Sanz P., Baulac S. Origin of laforin/ EPM2A according pubmed 14532330 (following cited reference 10 in publication) stephanie.baulac@upmc.fr J. Mol. Med. (0946-2716) 2011 imex curation Only protein-protein interactions nhlc1_human E3 ubiquitin-protein ligase NHLRC1 NHLRC1 EPM2B Malin NHL repeat-containing protein 1 RING-type E3 ubiquitin transferase NHLRC1 protein protein human Homo sapiens Human MAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVVCLACVAALAHPRTLALECPFCRRACRGCDTSDCLPVLHLIELLGSALRQSPAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGRVVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLVIGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLEHPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPEEFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWG epm2a_human Laforin EPM2A Lafora PTPase Glycogen phosphatase Glucan phosphatase protein protein human Homo sapiens Human MRFRFGVVVPPAVAGARPELLVVGSRPELGRWEPRGAVRLRPAGTAAGDGALALQEPGLWLGEVELAAEEAAQDGAEPGRVDTFWYKFLKREPGGELSWEGNGPHHDRCCTYNENNLVDGVYCLPIGHWIEATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLKHELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWMPTPDMSTEGRVQMLPQAVCLLHALLEKGHIVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPAVYIDEEALARAQEDFFQKFGKVRSSVCSL epm2a-nhlrc1-11 1776185 n/a 1776187 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NHLRC1/EPM2B/malin EPM2 mutations cy46tyr pro69ala,asp146asn and leu261pro also colocalize n/a 1776188 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 5 epm2a-nhlrc1-14 1776186 n/a 1776187 unspecified role unspecified role prey prey Q6VVB1:p.Pro69Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Variant in EPM2 (Epilepsy, progressive myoclonic 2; MIM:254780); dbSNP:rs28940576; UniProt FTId:VAR_019484 Q6VVB1:p.Asp146Asn mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Variant in EPM2 (Epilepsy, progressive myoclonic 2; MIM:254780); UniProt FTId:VAR_019487 Q6VVB1:p.Cys46Tyr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Variant in EPM2 (Epilepsy, progressive myoclonic 2; MIM:254780); UniProt FTId:VAR_070793 Q6VVB1:p.Leu261Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Variant in EPM2 (Epilepsy, progressive myoclonic 2; MIM:254780); UniProt FTId:VAR_070794 n/a 1776188 unspecified role unspecified role bait bait physical association physical association false false false 6A