IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ du-2008-3 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Du J., Huang C., Zhou B., Ziegler SF. A 548 amino acid sequence was also identified in this screen but could not be matched to a known isoform. sziegler@benaroyaresearch.org In vitro-generated human CD4+CD25+ Treg cell Journal of immunology (Baltimore, Md. : 1950) 2008 imex curation Only protein-protein interactions du-2008-5 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Du J., Huang C., Zhou B., Ziegler SF. sziegler@benaroyaresearch.org Journal of immunology (Baltimore, Md. : 1950) 2008 imex curation Only protein-protein interactions du-2008-6 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Du J., Huang C., Zhou B., Ziegler SF. sziegler@benaroyaresearch.org Journal of immunology (Baltimore, Md. : 1950) 2008 imex curation Only protein-protein interactions du-2008-4 human-jurkat Homo sapiens jurkat Human T cell leukemia anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Du J., Huang C., Zhou B., Ziegler SF. sziegler@benaroyaresearch.org Journal of immunology (Baltimore, Md. : 1950) 2008 imex curation Only protein-protein interactions foxp3_human Forkhead box protein P3 Scurfin FOXP3 IPEX JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP p35398-2 Nuclear receptor ROR-alpha RORA NR1F1 RZRA Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Alpha-1 protein protein human Homo sapiens Human MESAPAAPDPAASEPGSSGADAAAGSRETPLNQESARKSEPPAPVRRQSYSSTSRGISVTKKTHTSQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSNYIDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG p35398-1 Nuclear receptor ROR-alpha RORA NR1F1 RZRA Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Alpha-2 protein protein human Homo sapiens Human MNEGAPGDSDLETEARVPWSIMGHCLRTGQARMSATPTPAGEGARRDELFGILQILHQCILSSGDAFVLTGVCCSWRQNGKPPYSQKEDKEVQTGYMNAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSNYIDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG Produced by alternative promoter usage. Region from 23 to 71 inhibits DNA-binding and transactivation activity. Variant in position: 18:P->S (in a colorectal cancer sample, somatic mutation). p35398-4 Nuclear receptor ROR-alpha RORA NR1F1 RZRA Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Alpha-4 protein protein human Homo sapiens Human MMYFVIAAMKSQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSNYIDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG Produced by alternative promoter usage. Ref.2 (AAA02963) sequence is in conflict in position: 7:A->E. rora_human Nuclear receptor ROR-alpha Retinoid-related orphan receptor-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RORA NR1F1 RZRA RAR-related orphan receptor A protein protein human Homo sapiens Human MESAPAAPDPAASEPGSSGADAAAGSRETPLNQESARKSEPPAPVRRQSYSSTSRGISVTKKTHTSQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTELHDDLSNYIDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG foxp3-rora-2 1861028 N/A 1861032 unspecified role unspecified role bait bait n-terminus foxp3hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1861033 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A, Fig. 1B. foxp3-rora-1 1861028 N/A 1861034 unspecified role unspecified role prey prey N/A 1861032 unspecified role unspecified role bait bait n-terminus foxp3hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A, Fig. 1B foxp3-rora-3 1861028 N/A 1861035 unspecified role unspecified role prey prey N/A 1861032 unspecified role unspecified role bait bait n-terminus foxp3hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A, Fig. 1B rora4-foxp3-4 1861029 N/A 1861032 unspecified role unspecified role prey prey N/A 1861035 unspecified role unspecified role bait bait n-terminus ror-alphaa gal4 dna bd gal4 dna binding domain gal4 dna bd n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region rora4 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4C (top blot, lane 1) rora4-foxp3-5 1861029 N/A 1861032 unspecified role unspecified role prey prey region hu_fox3-1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false Q9BZS1:p.Leu95_Leu96delinsAlaAla foxp3 mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 1861035 unspecified role unspecified role bait bait region ror-alpha-4 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminus ror-alphaa flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P35398-4:p.Leu455_Phe456delinsAlaAla rora4 mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2A (top blot, lane 3), Fig. 3B (top blot, lane3), Fig. 4E (top blot, lane 4), Fig. 5A (top blot, lane 2, lane 4) foxp3-rora-4 1861030 N/A 1861032 unspecified role unspecified role bait bait region foxp3hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1861036 unspecified role unspecified role prey prey region rora-hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 1861067 1861065 physical association physical association false false false Fig. 3A foxp3-rora-5 1861030 N/A 1861035 unspecified role unspecified role prey prey region ror-alpha-4 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 1861032 unspecified role unspecified role bait bait n-terminus foxp3hu sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4A, Fig. 4B foxp3-1 1861031 N/A 1861035 unspecified role unspecified role bait bait region ror-alpha-4 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1861032 unspecified role unspecified role prey prey physical association physical association false false false Fig1.C, (top panel lane 1)