UniProt UniProt http://www.uniprot.org mancini-2000-1 The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mancini A., Koch A., Stefan M., Niemann H., Tamura T. Experiments/interactions involving phosphatidylinositol 3-kinase 85 kDa regulatory subunit (fig 2A and 4) omitted as protein identity is ambiguous and protein cannot be mapped to UniProt. Interactions involving Grb10 (fig 4) omitted as protein source and identity cannot be established. tamura.teruko@mh-hannover.de 10-11-day mouse embryo cDNA FEBS Lett. (0014-5793) 2000 imex curation Only protein-protein interactions mancini-2000-2 The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mancini A., Koch A., Stefan M., Niemann H., Tamura T. Experiments/interactions involving phosphatidylinositol 3-kinase 85 kDa regulatory subunit (fig 2A and 4) omitted as protein identity is ambiguous and protein cannot be mapped to UniProt. Interactions involving Grb10 (fig 4) omitted as protein source and identity cannot be established. tamura.teruko@mh-hannover.de FEBS Lett. (0014-5793) 2000 imex curation Only protein-protein interactions mancini-2000-3 The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mancini A., Koch A., Stefan M., Niemann H., Tamura T. Experiments/interactions involving phosphatidylinositol 3-kinase 85 kDa regulatory subunit (fig 2A and 4) omitted as protein identity is ambiguous and protein cannot be mapped to UniProt. Interactions involving Grb10 (fig 4) omitted as protein source and identity cannot be established. tamura.teruko@mh-hannover.de FEBS Lett. (0014-5793) 2000 imex curation Only protein-protein interactions mancini-2000-4 The direct association of the multiple PDZ domain containing proteins (MUPP-1) with the human c-Kit C-terminus is regulated by tyrosine kinase activity. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mancini A., Koch A., Stefan M., Niemann H., Tamura T. Experiments/interactions involving phosphatidylinositol 3-kinase 85 kDa regulatory subunit (fig 2A and 4) omitted as protein identity is ambiguous and protein cannot be mapped to UniProt. Interactions involving Grb10 (fig 4) omitted as protein source and identity cannot be established. tamura.teruko@mh-hannover.de 10-11-day mouse embryo cDNA FEBS Lett. (0014-5793) 2000 imex curation Only protein-protein interactions mpdz_mouse Multiple PDZ domain protein Mpdz Mupp1 Multi-PDZ domain protein 1 protein protein mouse Mus musculus Mouse MLETIDKNRALQAAERLQSKLKERGDVANEDKLSLLKSVLQSPLFSQILNLQTSLQQLKDQVNIATLATAAADHAHTPQFSSAVISNLQSESLLLSPNHGNLEALPGPGAPAVMDGKPTCDELDQLIKNMAQGRHVEIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIARGSLPPVSSPRISRSPSAASTISAHSNPMHWQHVETIELVNDGSGLGFGIIGGKATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPASSSLGITLSSSTSSTSEMRVDASTQKNDESETFDVELTKNVQGLGITIAGYIGDKKLEPSGIFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLMRKGASQEAELTSRGDTAKDVDLPAENCEKDEESLSLKRNTSILPIEEEGFPLLSAELEEAEDVQQEAALLTKWQRIMGINYEIVVAHVSKFSENSGLGISLEATVGHHFIRSVLPEGPVGHSGKLFSGDELLEVNGINLLGENHQDVVNILKELPIDVTMVCCRRTVPPIALSEMDSLDINDLELTEKPHIDLGEFIGSSETEDPMLAMSDVDQNAEEIQTPLAMWEAGGQSIELEKGSRGLGFSILDYQDPIDPANTVIVIRSLVPGGIAEKDGRLFPGDRLMFVNDINLENSTLEEAVEALKGAPSGMVRIGVAKPLPLSPEEGYVSAKEDAFLCSPHACKESGLSDKALFRADLALIDTPDAESIAESRFESQFSPDNDSVYSTQASIFSLHDGTCSDGMNYGPSLPSSPPKDVTSSSEVVLGLHLSLEELYTQNLLQRQHAGSPPTDMRPAPTSGFPISDYTTTNAVEQKYECANPVAWPHSQLPSSLSTSELAPALPAVAQKYLTDQSSLASDAESVNLQSMSQEAFERTVTIAKGSSSLGMTVSANKDGLGVIVRSIIHGGAISRDGRIAVGDCILSINEESTISLTNAQARAMLRRHSLIGPDIKITYVPAEHLEEFRVSFGQQAGGIMALDIFSSYTGRDIPELPEREEGEGEESELQNAAYSSWSQPRRVELWREPSKSLGISIVGGRGMGSRLSNGEVMRGIFIKHVLEDSPAGKNGTLKPGDRIIEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQSIINRPRKSPLPSLPHSLYPKYSFSSTNPFADSLQLTTDQAPSQSESETEKPALCNVPPSSPSVFSEMGSDCAQPSATAVSEDEDKEDEFGYSWKNIQERYGSLTGQLHVIELEKGQSGLGLSLAGNKDRTRMSVFIVGIDPTGAAGRDGRLQIADELLEINGQILYGRSHQNASSIIKCAPSKVKIIFIRNADAVNQMAVCPGIAADSPSSTSDSPQNKEVEPCSTTSASAADLSSLTDVYQLELPKDQGGLGIAICEEDTINGVMIESLTEHGGAAKDGRLKPGDHILAVDDEVVAGCPVEKFISLLKTAKATVKLTVRAENPACPAVPSSAVTVSGERKDNSQTPAVPAPDLEPIPSTSRSSTPAVFASDPATCPIIPGCETTIEISKGQTGLGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQRVRLTLYRDEAPYKEEDVCDTFTIELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRHATQEAVAALLKCSLGAVTLEVGRVKAAPFHSERRPSQSSQVSESSLSSFTPPLSGINTSESLESNSKKNALASEIQGLRTVEIKKGPADSLGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNASGSIEVQVVAGGDVSVVTGHQQELANPCLAFTGLTSSSIFPDDLGPPQSKTITLDRGPDGLGFSIVGGYGSPHGDLPIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMVLS mint Interacts with HTR2C and provokes its clustering at the cell surface. Member of the NMDAR signaling complex that may play a role in control of AMPAR potentiation and synaptic plasticity in excitatory synapses kit_human Mast/stem cell growth factor receptor Kit Tyrosine-protein kinase Kit KIT Proto-oncogene c-Kit SCFR Piebald trait protein p145 c-kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog protein protein human Homo sapiens Human MRGARGAWDFLCVLLLLLRVQTGSSQPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKGNNKEQIHPHTLFTPLLIGFVIVAGMMCIIVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPNRQKPVVDHSVRINSVGSTASSSQPLLVHDDV kit-mpdz-1 1768243 n/a 1768247 unspecified role unspecified role prey prey pdz_10 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1768248 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false physical association physical association false false false 1A mpdz-kit 1768244 n/a 1768247 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false n/a 1768248 unspecified role unspecified role bait bait val976del required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 2B mpdz-kit-1 1768245 n/a 1768247 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz_10 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1768248 unspecified role unspecified role prey prey human-m07e Homo sapiens hematopoietic cells m07e human hematopoietic cells physical association physical association false false false 3 kit-mpdz-2 1768246 n/a 1768247 unspecified role unspecified role prey prey pdz_10 sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1768248 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false val976del required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false P10721:p.Lys623Met mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false kinase negative mutant P10721:p.Asp816Val mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false In mast cell leukemia and mastocytosis. UniProt FTId: VAR_004109 1768271 1768269 physical association physical association false false false 2A