IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ viollet-1996-1 Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa tag visualisation tag visualisation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. Only the in vitro experiments were curated as in vivo the bands could potentially come from any protein binding to the MLP probe. J. Biol. Chem. (0021-9258) 1996 Only protein-protein interactions imex curation viollet-1996-2 Immunochemical characterization and transacting properties of upstream stimulatory factor isoforms. in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift tag visualisation tag visualisation Viollet B., Lefrançois-Martinez A.M., Henrion A., Kahn A., Raymondjean M., Martinez A. Only the in vitro experiments were curated as in vivo the bands could potentially come from any protein binding to the MLP probe. Fig. 5b, lane 12, has not been curated as the antibody recognises both isoforms, USF2a and USF2b, but the band cannot distinguish which homo- or heterodimer were in fact supershifted. J. Biol. Chem. (0021-9258) 1996 Only protein-protein interactions imex curation mlp-dsdna probe MLP-dsDNA probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) GTAGGCCACGTGACCGGGT usf1_human Upstream stimulatory factor 1 Major late transcription factor 1 USF1 USF BHLHB11 Class B basic helix-loop-helix protein 11 protein protein human Homo sapiens Human MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN q15853-3 Upstream stimulatory factor 2 USF2 BHLHB12 Class B basic helix-loop-helix protein 12 FOS-interacting protein Major late transcription factor 2 Upstream transcription factor 2 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ q15853-1 Upstream stimulatory factor 2 USF2 BHLHB12 Class B basic helix-loop-helix protein 12 FOS-interacting protein Major late transcription factor 2 Upstream transcription factor 2 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ usf2_human Upstream stimulatory factor 2 USF2 Upstream transcription factor 2 FOS-interacting protein Major late transcription factor 2 BHLHB12 Class B basic helix-loop-helix protein 12 protein protein human Homo sapiens Human MDMLDPGLDPAASATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAITSVQQAAFGDHNIQYQFRTETNGGQVTYRVVQVTDGQLDGQGDTAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPPGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCNADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGEGTRQ mlp-usf1-1 1536492 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 physical association physical association false false false Fig. 2b, lanes 3, 8, 9 & 10; Fig. 5b, lanes 1, 4, 5, 7 & 8 mlp-usf1-usf2b-1 1536492 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lane 3; Fig. 5b, lane 7 mlp-usf2b-1 1536492 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lanes 2 & 3; Fig. 5b, lanes 3, 7, 9, 10 & 11 mlp-usf2a-usf2b-1 1536492 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lane 2; Fig. 5b, lane 10 mlp-usf2a-1 1536492 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lanes 2, 9 & 11; Fig. 5b, lanes 2, 4, 6 & 10 mlp-usf1-2 1536492 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Fig. 5a, lanes 7, 9 & 10 mlp-usf2a-2 1536492 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 5a, lanes 1, 2, 6, 8 & 9 mlp-usf1-usf2a-1 1536492 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lane 9; Fig. 5b, lane 4 mlp-usf1-usf2-1 1536492 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536498 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lane 10 mlp-usf2-1 1536492 n/a 1536498 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey myc-type, basic helix-loop-helix (bhlh) domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 2b, lanes 10 & 11 mlp-usf2b-2 1536492 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 5a, lanes 2-5 mlp-usf2a-usf2b-2 1536492 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false Fig. 5a, lanes 1, 2 & 4 mlp-usf1-usf2a-2 1536492 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 5a, lanes 8-10 mlp-usf2a-3 1536493 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536497 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 physical association physical association false false false Fig. 5b, lanes 5 & 11 mlp-usf1-3 1536493 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1536495 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 physical association physical association false false false Fig. 5b, lanes 6 & 9 mlp-usf2b-3 1536493 n/a 1536496 weight identificat confirmation by molecular weight weight identificat unspecified role unspecified role prey prey rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 2.0 n/a 1536494 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Fig. 5b, lane 8