bhf-ucl huang-1995a-1 ApoE3 binding to tau tandem repeat I is abolished by tau serine262 phosphorylation. in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis western blot western blot Immuno blot Immuno blot Huang D.Y., Weisgraber K.H., Goedert M., Saunders A.M., Roses A.D., Strittmatter W.J. Cardiac - Interactions involving cardiac related proteins Human tau peptides were incubated with either ApoE3 or ApoE4. After the incubation, proteins were submitted to electrophoresis and transferred to a membrane that was incubated with the anti-tau serum 135 primary antibody. Apo E purified from human plasma confirmed in reference 19: Rall S.C. et al 1986 (PMID: 3724506) warren@neuro.duke.edu Neurosci. Lett. (0304-3940) 1995 Only protein-protein interactions imex curation p02649-pro_0000001987 Apolipoprotein E APOE protein protein human Homo sapiens Human KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 19 317 tau_human Microtubule-associated protein tau MAPT MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau MAPTL protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL apoe-mapt-1 1480702 n/a 1480703 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma n/a 1480704 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P10636:p.Cys639Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false phosphoserine 579 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false Phosphorylation of Serine 579 of Tau protein peptide I completely disrupts its interaction with ApoE3 chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig 1, 2, 3 apoe-mapt-2 1480702 n/a 1480704 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1480703 unspecified role unspecified role neutral component neutral component cys130arg variant variant certain certain sequence position certain certain certain sequence position certain false human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction false false false Fig 1 apoe-apoe-4 1480702 n/a 1480704 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P10636:p.Cys639Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1480703 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma n/a 1480703 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma physical association physical association false false false Fig 2