IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ randhawa-1983-1 Separation of zeta (zeta) and various gamma (gamma) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography. human-erythrocyte Homo sapiens erythrocyte Erythrocyte, Red blood cell; Corpuscle; RBC electrophoresis electrophoretic mobility-based method electrophoresis predetermined predetermined participant predetermined Randhawa Z.I., Jones R.T., Lie-Injo L.E. HPLC. Haemoglobin was obtained from normal adults and neonates with Hydrops fetalis due to homozygous a-Thalassaemia. Anal. Biochem. (0003-2697) 1983 Only protein-protein interactions imex curation hbb_human Hemoglobin subunit beta Hemoglobin beta chain Beta-globin HBB protein protein human Homo sapiens Human MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH heme b Heme b small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR hbg1_human Hemoglobin subunit gamma-1 HBG1 Hemoglobin gamma-1 chain Gamma-1-globin Hemoglobin gamma-A chain Hb F Agamma PRO2979 protein protein human Homo sapiens Human MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTAVASALSSRYH hbg2_human Hemoglobin subunit gamma-2 HBG2 Gamma-2-globin Hb F Ggamma Hemoglobin gamma-2 chain Hemoglobin gamma-G chain protein protein human Homo sapiens Human MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFGKEFTPEVQASWQKMVTGVASALSSRYH hbaz_human Hemoglobin subunit zeta Hemoglobin zeta chain Zeta-globin HBAZ HBZ HBZ2 protein protein human Homo sapiens Human MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAARFPADFTAEAHAAWDKFLSVVSSVLTEKYR hba1-hbb-1 1498978 n/a 1498979 unspecified role unspecified role neutral component neutral component n/a 1498980 unspecified role unspecified role neutral component neutral component n/a 1498981 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1a hbg1-hbg2 1498978 n/a 1498982 unspecified role unspecified role neutral component neutral component n/a 1498980 unspecified role unspecified role neutral component neutral component n/a 1498983 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1b, 2b, 2c hbg1-hbz 1498978 n/a 1498982 unspecified role unspecified role neutral component neutral component n/a 1498983 unspecified role unspecified role neutral component neutral component n/a 1498980 unspecified role unspecified role neutral component neutral component n/a 1498984 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 1c hbg1-hbz-2 1498978 n/a 1498983 unspecified role unspecified role neutral component neutral component n/a 1498982 unspecified role unspecified role neutral component neutral component n/a 1498979 unspecified role unspecified role neutral component neutral component n/a 1498984 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 3 Amino acid composition for zeta chain was determined by amino acid analysis. HPLC analysis of a non pre-purified sample can be found in Fig. 4.