MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014za-2 Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined karen.glass@acphs.edu 2014 imex curation Lubula M.Y., Eckenroth B.E., Carlson S., Poplawski A., Chruszcz M., Glass K.C. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014q-1 Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined karen.glass@acphs.edu 2014 imex curation Lubula M.Y., Eckenroth B.E., Carlson S., Poplawski A., Chruszcz M., Glass K.C. FEBS Lett. (0014-5793) Only protein-protein interactions brpf1_human Peregrin BRPF1 BR140 Bromodomain and PHD finger-containing protein 1 Protein Br140 protein protein human Homo sapiens Human MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQSRPANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTETPAATPKSGKHKNKEKRKDSNHHHHHNVSASTTPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEDEDEEEDEGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQVGRDSEDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQIAMEMQLTPFLILLRKTLEQLQEKDTGNIFSEPVPLSEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLIVSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRESQMTPSHGGSPVGPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGFSGGNQPVKKSFLVYRNDCSLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTSGTENEAYSVGTGRGVGHSMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYPALIIDPKMPREGMFHHGVPIPVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGVNQDLDKEKMLEGRKSNIRKSVQIAYHRALQHRSKVQGEQSSETSDSD h2a1_human Histone H2A type 1 H2AC11 H2AC13 H2AC15 H2AC16 H2AC17 H2AFC H2AFD H2AFI H2AFN H2AFP HIST1H2AG HIST1H2AI HIST1H2AK HIST1H2AL HIST1H2AM Histone H2A/ptl protein protein human Homo sapiens Human MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK h4_human Histone H4 H4-16 H4C1 H4C11 H4C12 H4C13 H4C14 H4C15 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A HIST2H4B HIST4H4 protein protein human Homo sapiens Human MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG brpf1-hist1h2ag 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3a sf1a brpf1-hist1h2ag-1 1568127 n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Ala709Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3a sf1g brpf1-hist1h2ag-2 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P55201:p.Glu661Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3a sf1d brpf1-hist1h2ag-3 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Ile713Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3a sf1f brpf1-hist1h2ag-4 1568127 n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Cys704Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3a sf1e brpf1-hist1h2ag-5 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Phe714Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f3a sf1c brpf1-hist1h2ag-6 1568127 n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Asn708Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f3a sf1b brpf1-hist1h4a-2 1568127 n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3b f3c sf2a sf3a No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-3 1568127 n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Ala709Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3b f3c sf2g sf3g No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-4 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P55201:p.Glu661Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3b f3c sf2d sf3d No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-5 1568127 n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P55201:p.Ile713Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3b f3c sf2f sf3f No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-6 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Cys704Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1568127 f3b f3c sf2e sf3e No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-7 1568127 n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P55201:p.Phe714Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f3b f3c sf2c sf3c No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a-8 1568127 n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P55201:p.Asn708Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f3b f3c sf2b sf3b No binding was observed between BRPF1 and H4(4-17) unmodified peptide. brpf1-hist1h4a 1568128 n/a H4K12ac 1568131 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys12 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false t1 f2b f2d brpf1-hist1h4a-1 1568128 n/a H2AK5ac 1568130 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified naclys5 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a BRPF1 1568129 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false T1 f1c f1d f2a f2c