MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014e-1 Biophysical and structural characterization of a folded core domain within the proregion of Growth and Differentiation Factor-5 (GDF5) in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined elisabeth.schwarz@biochemtech.uni-halle.de 2014 imex curation Thieme T., Patzschke R., Job F., Liebold J., Seemann P., Lilie H., Balbach J., Schwarz E. FEBS J. (1742-464X) Only protein-protein interactions gdf5_human Growth/differentiation factor 5 GDF5 CDMP1 Cartilage-derived morphogenetic protein 1 Radotermin BMP14 Bone morphogenetic protein 14 Lipopolysaccharide-associated protein 4 protein protein human Homo sapiens Human MRLPKLLTFLLWYLAWLDLEFICTVLGAPDLGQRPQGTRPGLAKAEAKERPPLARNVFRPGGHSYGGGATNANARAKGGTGQTGGLTQPKKDEPKKLPPRPGGPEPKPGHPPQTRQATARTVTPKGQLPGGKAPPKAGSVPSSFLLKKAREPGPPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPVVRKQRYVFDISALEKDGLLGAELRILRKKPSDTAKPAAPGGGRAAQLKLSSCPSGRQPASLLDVRSVPGLDGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFDRAARQVHEKALFLVFGRTKKRDLFFNEIKARSGQDDKTVYEYLFSQRRKRRAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR mint gdf5-gdf5 1565372 n/a proGDF5 1565373 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a mature GDF5 1565373 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1565372 1565372 f7a part. In order to address this assumption via an independent experimental approach, the interaction of the proregion with the mature part was analyzed in SPR experiments. Mature GDF5 was immobilized and the proregion used as a soluble analyte. Association of the wild type proregion with mature GDF5 deviated from a 1 : 1 interaction. The curve shape was interpreted according to two binding reactions and one association constant with KD = 15.1 nM and the second one with KD = 911 nM. It is possible that the low affinity interaction reflects the association of the proregion with the mature part that has already bound one molecule of proregion. Association of GDF5 with the T201P proregion occurred at clearly reduced affinities of KD = 304 nM and KD = 7.2 μM. gdf5-gdf5-1 1565372 n/a mature GDF5 1565373 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a proGDF5 1565373 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified P43026:p.Thr201Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1565372 1565372 f7b part. In order to address this assumption via an independent experimental approach, the interaction of the proregion with the mature part was analyzed in SPR experiments. Mature GDF5 was immobilized and the proregion used as a soluble analyte. Association of the wild type proregion with mature GDF5 deviated from a 1 : 1 interaction. The curve shape was interpreted according to two binding reactions and one association constant with KD = 15.1 nM and the second one with KD = 911 nM. It is possible that the low affinity interaction reflects the association of the proregion with the mature part that has already bound one molecule of proregion. Association of GDF5 with the T201P proregion occurred at clearly reduced affinities of KD = 304 nM and KD = 7.2 μM.