IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ dubois-2014-2 Remodeling of Channel-Forming ORAI Proteins Determines an Oncogenic Switch in Prostate Cancer. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dubois C., Vanden Abeele F., Lehen'kyi V., Gkika D., Guarmit B., Lepage G., Slomianny C., Borowiec A.S., Bidaux G., Benahmed M., Shuba Y., Prevarskaya N. Cancer - Interactions investigated in the context of cancer natacha.prevarskaya@univ-lille1.fr,Fabien.vanden-abeele@inserm.fr Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation dubois-2014-1 Remodeling of Channel-Forming ORAI Proteins Determines an Oncogenic Switch in Prostate Cancer. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Dubois C., Vanden Abeele F., Lehen'kyi V., Gkika D., Guarmit B., Lepage G., Slomianny C., Borowiec A.S., Bidaux G., Benahmed M., Shuba Y., Prevarskaya N. Cancer - Interactions investigated in the context of cancer natacha.prevarskaya@univ-lille1.fr,Fabien.vanden-abeele@inserm.fr Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation orai3_human Protein orai-3 ORAI3 TMEM142C Transmembrane protein 142C protein protein human Homo sapiens Human MKGGEGDAGEQAPLNPEGESPAGSATYREFVHRGYLDLMGASQHSLRALSWRRLYLSRAKLKASSRTSALLSGFAMVAMVEVQLESDHEYPPGLLVAFSACTTVLVAVHLFALMVSTCLLPHIEAVSNIHNLNSVHQSPHQRLHRYVELAWGFSTALGTFLFLAEVVLVGWVKFVPIGAPLDTPTPMVPTSRVPGTLAPVATSLSPASNLPRSSASAAPSQAEPACPPRQACGGGGAHGPGWQAAMASTAIMVPVGLVFVAFALHFYRSLVAHKTDRYKQELEELNRLQGELQAV crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA stim1_human Stromal interaction molecule 1 STIM1 GOK protein protein human Homo sapiens Human MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK orai3-orai1 1562681 n/a 1562683 unspecified role unspecified role bait bait n/a 1562684 unspecified role unspecified role prey prey physical association physical association false false false 6 E, orai1-orai3-1 1562681 n/a 1562683 unspecified role unspecified role prey prey n/a 1562684 unspecified role unspecified role bait bait physical association physical association false false false 6 E/F Thapsigargin (CHEBI:9516) - a calcium pump inhibitor Thapsigargin (CHEBI:9516) plus arachidonic acid (CHEBI:15843) orai1-orai3-2 1562681 n/a 1562684 unspecified role unspecified role bait bait n/a 1562685 unspecified role unspecified role prey prey physical association physical association false false false Supp figure 4E, 5J, 6I Thapsigargin (CHEBI:9516) - a calcium pump inhibitor ORAI3 does not coimmunoprecipitate with STIM1. ORAI3 overexpression reduces ORAI1 coimmunoprecipitation with STIM1 under TG exposure. orai1-orai3 1562682 n/a 1562683 unspecified role unspecified role neutral component neutral component n/a 1562684 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 6 A/B