MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014s-1 Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence takashi.toda@cancer.org.uk 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014za-2 Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot takashi.toda@cancer.org.uk 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. FEBS Lett. (0014-5793) Only protein-protein interactions anonymous-2014za-3 Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot takashi.toda@cancer.org.uk 2014 imex curation Tang N.H., Okada N., Fong C.S., Arai K., Sato M., Toda T. FEBS Lett. (0014-5793) Only protein-protein interactions alp7_schpo Microtubule protein alp7 Altered polarity protein 7 Transforming acidic coiled-coil protein mia1 alp7 mia1 SPAC890.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDIVSSSTDYSRRSPSSSSIGTNETDHTGFHEKRQGASSESLIPPAQRSSEESMPAPKLFPKLTSKPNPQLNLKDTLNKRVSDRLQALELNKSFDFSGTPRPMHPISHPLSQHKTPEFKHRKRNVESILTPKNPSLFSSSNAASQRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSNGSFPRQTNTAPLNPPVHLKDNIRNSATPSTSQADIPTQYPINSTQKQQAKYEAEIEGYKAKLAGTYHEISVLQNTIVNVSGQLIAVNDQLQQLRSGKASTSPSTKDTNMRLVEGHNEETLALQRGKYTQEEVDKLIQERMEKVAEDLHAQYSAKHTQKINAFKANYARKYEATIQELQNQIGTAPNAPKISNSNWEEERRALKADNQTLQKQLEKAIQERQDMSDFLNNFKADMAKSDKLLMQQQSQQTGDLETLRLQLQALQEELRVEREERQQLIQMSEDLVIAMDQLNLEQKS 22B4216C196E310C alp14_schpo Spindle pole body component alp14 Altered polarity protein 14 alp14 mtc1 SPCC895.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSQDQEEDYSKLPLESRIVHKVWKVRLSAYEECSKSFSLSADGSDNCFELWNNQSELWKSVLTDSNVAAQEAGTAAFVAYCRFSDPSHLLKAREISVLSISEKCLTSPRAGTRENALEALMLLVEADSAAPVIESIIPSLSARSPKVIASNVAAIASLVEQFGAKVIPSKMIIPHISNLFGHADKNVRKEASRLTVNIYRWTGDPLKDLLFKDLRPVQTKELESLFAELPTEPPKQTRFLKSQQPTSEPNVETQVEEQPALENEESEPEPSDDQFDLVEEVDVLPNVDPNLETLMASSKWKDRKEALDKLLPVLSQPKIKDNDFFNLVAILTKSVSKDANIMVVINAAHCIQAMAKGLRSNFSKYASTSINALLERSKEKKANVIESLSSAMDAVLATSSLDDLAELIASFAGNKNPQIKSSCFSLFSRSFSNMTSLPSKFTVDTCAKACVPGVSDTFEPVRSAAAEALGVLMKLVGERAINQYLSPLDDIRKSKIRSFYETATVKAKAPTKKSKVKPSKQEESKVVVPSNAKAVKKSVVPSSPVVPSPRKATNKSLSMDVSKGNAFENGPLLPRPTTRPVSRGLSRGTSSSLQQKVKASTPLNSGALNETVQNLKNMELDDPAPQPAKHSRVDRYEHPKVLEDNDSTISSLESLKRENEELREQLKVEHEENISMQKQLSELKGELNTLRSARKASPIGDRKPAFMRRANTDFLELSTSPSFQRSVREFEPTRPKLYSSIDVNQRSPLASAKTNGNFTFHAELPRSPFSSRANNINPDWTKAIDLAAKLKQKITEMKQTDQRHQGLIH 78B2027F0EC9A939 alp4_schpo Spindle pole body component alp4 Altered polarity protein 4 alp4 SPBC365.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVRSISSVLAKEESENGSPIPALEEGSVYHVSLKSEGVSPFSDRIQLNYLYDGKTFSDPSNLNIHQQEACLINELLNAFMGMEGVFVHLQDMKASSEFETIIMPPSFYILPGFDLGIKDIASEMLEMGSHYLSITAFIESRSHFEYGFVNHALCAALRKFVMDYVVLIMQCENQSRIDPNFSLQTLRLYTLPTSRSLRQVYLILRDLLLSMEKNASSSDDLGLSNIDDLLEQLNEGNDISHVVNATRSKKKVCKGGQVISFLTESLTKYAGDPVARKILTYLLREASRPYTKMLNEWIHLGLVNDPYDEFMIKIHKGITSMQLDEDYTDEYWEKRYVIREDQVPPQLLDLQNKVLFAGKYLNVVLECRKGVNNLASLNAKDDTQNQLLWPSTFDDDNFTLNIMNAYVYANESLLQLLQSSQSLYAHLYSLKHYFFLDQSDFFTTFLDNAQHELRKPAKYISITKLQSQLDLALRQPGTITATDPHKEYVTVEVNQTSLIDWLMHIVSISGLEEGTSSQGNEVWNESITKQADVGNETRNFESEHNRSTQGTSKVGSDKDINGFETMQLCYKVPFPLSLILSRKAIIRYQLLFRYFLLLRHVEMQLENSWVQHSKNSAWRLNSSNAKIEQWKRNSWLLRTRMLSFVQKIIYYTTSEVIETHWGKFMGELENARTVDNLMQEHIDFLDTCLKECMLTNSRLLKVQSKLLNTCAMFASYTSTFTRSLYLLENSEESFDEGRMDKMEEILRRYEDSFSRHLKSLVNACNYFASTETAALLSLVMKLTG pcp1_schpo Spindle pole body protein pcp1 pcp1 SPAC6G9.06c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSERDFNTQSPKFKDENANSVISQSDFLGKSLKNNNDDYSDFRGSYLNDKSSFQTPLRNGSYQPKGSLEFTPLLQSSNKNSDKYNGSLGDKGSFDPNSYGLSAISKQATQEALSISQGNDSYDVSKLTDLSKNSEIDHTDGELPANAALTLREQEKVLEKVSRENFGLRIKIVCLEKRLESMAPEQIKEAVKDNVELHAERANLQLQLKRTESLLQKSEDKNFKLEEKVDYLSKVNDVEQSQNVKVFTERIRFLENALEKVQREKDSLSTEMEEDKSNKEVDYEYEIRQLQNRLDELSEELDVAQDLLTEKEDEIATLKRQIEEKENSSSAFENEENSSYVHLQEDYAILQAKCDEFADRIQVLTADLEKEKENQIMHESEASIGLTDSMQVHTLQEQLHKANEEIEFLHDQISRMNEEGKNFEDIMLQFRSLEEERDVLESKLQTLEDDNNSLRLMTSSLGNQIESLRTQNREIDEEKNHLRLLASKNSDKALAETNIRLQEVTKELETLRMKNSNDLNEIHDLREENEGLTLKIDSITKEKDRLINELEQRIKSYEVNVSELNGTIDEYRNKLKDKEETYNEVMNAFQYKDNDLRRFHESINKLQDREKELTSNLEKKNLVISSLRETVAMLEKERESIKKYLSGNAKDLDNTNLMEILNDKISVLQRQLTDVKDELDVSEEEREEAIVAGQKLSASFELMSNEKQALELKYSSLKNELINAQNLLDRREEELSELSKKLFEERKIRSGSNDDIEKNKEINVLNSELADKLAQIRHLESDKMELDKLVHHLNRGIEEANIEENAVKKRLCLLMGCDYSSVSILQIVSQIEHFVNQQIQTIRSLKQELRHDFVQFSGKKEQELSRSFEKFGLGTETKHDILAQRNRNVSEKMNDLENAAQKFFSSPDRKNGYLYPSEHTSKIEYLEKTIEDLKLALQDELKNRNLLMDDISSYNKQTTKLQEKIKWLERERSILIDELESYRSNQFNYQNNLVQDKNELEERLKEIQKELEVYNNHFMKQAELMTSNVTDESQLMLKTLREALQSKTNNIDHLSTILERNRKEYKSLLDDYNQLRARYKNLQSNTPQSTQSGQYESEIKGLSKLTKYLQSKCRREHSLRLDLAFSKKFILMQLTGYETCNKINLRMLQKIGISPDPDLSKKHIKLKSLIIVVCSIERMKRMKNEWLKQAQLKQSLQRAAAKAKTANY mint Spindle pole body component that binds calmodulin. Overexpression of pcp1 causes the formation of supernumerary SPB-like structures and disrupts both mitotic spindle assembly and chromosome segregation alp14-alp7-5 1545751 n/a Alp7 1545754 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Alp14 1545755 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f2b alp14-alp7-6 1545751 n/a Alp7 1545754 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Alp4 1545756 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f2d alp7-pcp1 1545751 n/a Alp7 1545754 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell Q9URY2:p.[Lys341Ala;Tyr344Ala;Arg346_Tyr348delinsAlaAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Pcp1 1545757 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f2e alp7-pcp1-1 1545752 n/a Alp7 1545754 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell n/a Pcp1 1545757 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f4g pcp1-alp7 1545753 n/a Alp7 1545754 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell n/a Pcp1 1545757 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f4g