IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ p_rez_vargas-2014-1 Structural basis of eukaryotic cell-cell fusion. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pérez-Vargas J., Krey T., Valansi C., Avinoam O., Haouz A., Jamin M., Raveh-Barak H., Podbilewicz B., Rey F.A. rey@pasteur.fr, podbilew@technion.ac.il Cell (0092-8674) 2014 Only protein-protein interactions imex curation p_rez_vargas-2014-2 Structural basis of eukaryotic cell-cell fusion. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pérez-Vargas J., Krey T., Valansi C., Avinoam O., Haouz A., Jamin M., Raveh-Barak H., Podbilewicz B., Rey F.A. rey@pasteur.fr, podbilew@technion.ac.il Cell (0092-8674) 2014 Only protein-protein interactions imex curation g5eca1_caeel eff-1 C26D10.5 CELE_C26D10.5 protein protein caeel Caenorhabditis elegans MEPPFEWSPQFILLLLAVTTYGFPLEEKFDGLFRAEPPHCSKTPIVRAQTSQNAMSSIARGMQMQFSIGLHTAVCFRLYEDTQLASQEINDDENAGNQTSLLHTIRLEKLEHHHPITQRYTFGIPEVHASCICECDATSSTCTAESHQFTACPESDKSDETSSCYRTFFPNQTPIGCSEDDIPKLCCDVRFKPYKNMTFLAVKLEQPTTYATFVYAAYDFVNGYWVEKDKTKIRSQLDGGTQDRHLDQKRRISLAVTAGGRASHQLETGMYFSRTSNGGETEELRMQPLNEITDNNFDRLGWYRMDDSGHFHVNNGVVKMDDIHKAKVKNCKEQTYKSILSANHYMPGHFNLTRPLEVIKPWIQSARIFDSSLRQAVVTHAEGTNLQISIHLDDEVESQNLVFFHNASRIRDFSGSIIVDSKSNRLFNLTVYEASGKIDGSVKMSTGFGSDTIHTFTAYVSDLHASNRSMIIPLPAIVGQGARAICLRADSMADIDKICHVIEYFESPLEIDLVEGKWHEMIGTCPTCNQINFNGMMKFLNPAHWIKGISSIGDGVMIATDIVVYLGVLCILYLLITKIIVPLVRCWVCPMSIFCNGSSSSSKNKNDKRRKEREERRRKDKFVSESEDGARSSSEPHDTLARYHGNHSERHYSSSQYI eff-1 1539331 n/a 1539333 unspecified role unspecified role neutral component neutral component ecto region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Stoichiometry: 3.0 direct interaction direct interaction false false false Rworking = 0.22 Rfree = 0.28 2.9A Supp. Table 1 eff-1 1539331 n/a 1539333 unspecified role unspecified role neutral component neutral component ecto region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Stoichiometry: 3.0 direct interaction direct interaction false false false Rworking = 0.18 Rfree = 0.22 2.6A Supp. Table 1 eff-1 1539331 n/a 1539333 unspecified role unspecified role neutral component neutral component ecto region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G5ECA1:p.[Gly260Ala;Asp321_Asp322delinsGluGlu] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Stoichiometry: 3.0 direct interaction direct interaction false false false Rworking = 0.2 Rfree = 0.22 2.3A Supp. Table 1 eff-1 1539332 n/a 1539333 unspecified role unspecified role neutral component neutral component G5ECA1:p.[Gly260Ala;Asp321_Asp322delinsGluGlu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false G5ECA1:p.Asp321_Asp322delinsGluGlu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecto region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G5ECA1:p.Gly260Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Stoichiometry: 3.0 direct interaction direct interaction false false false Fig. 4B, Supp 6A