MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014t-1 Structural insights into bacterial resistance to cerulenin in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined schujman@ibr-conicet.gov.ar 2014 imex curation Trajtenberg F., Altabe S., Larrieux N., Ficarra F., de Mendoza D., Buschiazzo A., Schujman G.E. FEBS J. (1742-464X) Only protein-protein interactions fabf_bacsu 3-oxoacyl-[acyl-carrier-protein] synthase 2 fabF yjaY 3-oxoacyl-[acyl-carrier-protein] synthase II Beta-ketoacyl-ACP synthase II BSU11340 protein protein bacsu Bacillus subtilis MTKKRVVVTGLGALSPLGNDVDTSWNNAINGVSGIGPITRVDAEEYPAKVAAELKDFNVEDYMDKKEARKMDRFTQYAVVAAKMAVEDADLNITDEIAPRVGVWVGSGIGGLETLESQFEIFLTKGPRRVSPFFVPMMIPDMATGQISIALGAKGVNSCTVTACATGTNSIGDAFKVIQRGDADVMVTGGTEAPLTRMSFAGFSANKALSTNPDPKTASRPFDKNRDGFVMGEGAGIIVLEELEHALARGAKIYGEIVGYGSTGDAYHITAPAQDGEGGARAMQEAIKDAGIAPEEIDYINAHGTSTYYNDKYETMAIKTVFGEHAHKLAVSSTKSMTGHLLGAAGGIEAIFSILAIKEGVIPPTINIQTPDEECDLDYVPDEARRQELNYVLSNSLGFGGHNATLIFKKYQS fabf-1 1512861 n/a FabF 1512862 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false t2 f1 In order to understand the structural bases of cerulenin-resistance in the FabF[I108F] enzyme of B. subtilis, as well as the differential properties that have been reported for the same mutation in E. coli, we decided to solve the 3D structures of the B. subtilis enzymes. Both wtFabF and the FabF[I108F] point-mutant, crystallized in similar mother liquors resulting in isomorphous orthorhombic crystals, containing one dimer per asymmetric unit. The diffraction data allowed us to solve both crystal structures at high resolution, 1.8Å for wtFabF and 1.55Å for the ceruleninresistant FabF[I108F] (Table 2). Furthermore, we solved the structure of wtFabF in complex with cerulenin, both covalently and non-covalently (Table 2).