IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ blanco_s_nchez-2014-2 Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. danre-larvae Danio rerio Brachydanio rerio larva Immature form of an organism pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Blanco-Sánchez B., Clément A., Fierro J., Washbourne P., Westerfield M. Interactions involving Sec23 have not been included due to lack of antibody specificity. hair cells in the anterior macula of the inner ear. UniProt identifiers have been taken from the ZFIN database. monte@uoneuro.uoregon.edu Dis Model Mech (1754-8403) 2014 Only protein-protein interactions imex curation blanco_s_nchez-2014-1 Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. danre-larvae Danio rerio Brachydanio rerio larva Immature form of an organism confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Blanco-Sánchez B., Clément A., Fierro J., Washbourne P., Westerfield M. hair cells in the anterior macula of the inner ear. UniProt identifiers have been taken from the ZFIN database. monte@uoneuro.uoregon.edu Dis Model Mech (1754-8403) 2014 Only protein-protein interactions imex curation blanco_s_nchez-2014-3 Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Blanco-Sánchez B., Clément A., Fierro J., Washbourne P., Westerfield M. UniProt identifiers have been taken from the ZFIN database. monte@uoneuro.uoregon.edu Dis Model Mech (1754-8403) 2014 Only protein-protein interactions imex curation blanco_s_nchez-2014-4 Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Blanco-Sánchez B., Clément A., Fierro J., Washbourne P., Westerfield M. UniProt identifiers have been taken from the ZFIN database. monte@uoneuro.uoregon.edu Dis Model Mech (1754-8403) 2014 Only protein-protein interactions imex curation blanco_s_nchez-2014-5 Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Blanco-Sánchez B., Clément A., Fierro J., Washbourne P., Westerfield M. UniProt identifiers have been taken from the ZFIN database. monte@uoneuro.uoregon.edu Dis Model Mech (1754-8403) 2014 Only protein-protein interactions imex curation f1qvn7_danre ift88 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MENVHLVPEEEEDDLYTGYNDYNPTFDSEDLHNDVAFQQAVRTSHGRRPPMTAKYPGTAIGGRPIGTAYGSRVPIGTAMGRPMTGAVQDGAARPMTAVRAAGYSSSIARGSAFDPLGQAKGPAPPLETKNEDTPEEKIKILEKKVNDLIEDSCLAHARGDLQVSLEKAKEAGRKERALVRQREQTGTADHINLDLTYSVLFNLANQYANNDMYTEALNTYQVIVKNKMFNNAGRLKVNMANIYFKQKNYTKAIKFYRMALDQISNAHNAMRIKIMQNIGVVFIHMGQYSDAITSFEYIMTESPNIKTGFNLILCYYAIGDRERMKKAFQKLICVPLGVDDDDKYIPPNDDPHANMVIEAIKNDKLHQMERERKALAEKYIMTSAKLIAPAIEMSFAAGFDWCVDMVKGSQYVELANDLEINKAITYLRQRDFKQAVETLKMFEKKDSRVKSAAATNLSFLYFLEKDFDQADRYAELAMSADRYNPAALINKGNTLFVKEDYEKAAEFYKESLRNDSSCTEALYNLGLTYKRLGRLEEALDCFLKLHAILRNSAQVMYQLANLYEMLEDPHQAIEWLMQLTSVTPTDAQVLAKLGDLYDNEGDKSQAFQYYYESYRYFPSNISVIEWLGAYYIDTQFCEKAIQYFERATLIQPTQVKWQLMVASCYRRSGNYQKALETYKEIHRKFPENVECLRFLVRLCTDMGLKEVQDYATKLKKVEKMKEIREQRVRSGRDSSARSRREGSAGSGSSSSPINTPPLRASVSRSEVKYSPVLEQSRDSGQSNHGTSAKSERLSVKLKTLPGSNEPYEASTQQEIDASYVDPLGPQTQRPKTAARKRNEEDEFADEELGDDLLPE q29rc0_danre ush1c protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVTFF q6qqe1_danre cdh23 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MSWCEGSGGKMTWVKLLLSLVLLHAVSEASANQPPRFQNYFFQSYLLVYEDTPVGTSITQLQAVDPDGEPLIFGVVGEEAMRYFAVQGTTGVVWLRQPLDREAKSEMQVEFTVSDSQGVVKDTVNIQIGDVNDNAPSFYNQPYAIQIPENTPVGTSVFMVNATDPDQGVGGSVLFSFQPPSQFFSIDGARGIVTVTRALDYETTIAYQLTVNATDQDKRRPLSSLANLAITITDIQDMDPIFTNLPYSTNIMEDAPPGYEVRKIRAIDQDLGRPRGIGYTIISGNTNSMFALDYISGSLTVSGQLDRENPLYSSGFIITVKATELNDDRTPSSATVTTTFTILLIDKNDNPPRFNSSEYRVLITELAQVGFALPLYIQVEDKDEGVNSMFQVFLTGNNSDHFSISPTSVQGRADLRLRVAIPLDYETIRSYSFSLYANESLSEHVGFARVFIDLINENDNRPIFSQTLYNISLPESIEPGASLLQIKATDNDIGTFGVVRYYFSDEPDQFSLDAETGWVTLRGRLDYELMRRFTLTILARDGGGEETTGRLRINVMDVNDNAPVFQKEAYTGSLMENEQAPQQVIRARATDEDSPPNNILTYSIIYASQFRSFFSISIAEGYAVITVTRPLDYEQVPGGLIFLTLMAKDGGNPSLNSTVPVIIELIDQNDNPPEFSLPSYIVNIPENIVAGATVLFVNATDLDASREFGQTSLIYSLQGSSQFRLNTRSGEITTTALLDREMKSEYILIVRAVDGGVGPQQKTGIATVNITVLDINDNAPVWRDEPYQANVVEMSPIDTDVITVFAVDPDYGDNGTVVYSINPENPFYTINRNTGKIRTSGAVLDRESQDARSAQLMRTIIISATDRGTPPLRSSASATVFVNLLDLNDNDPAFLNLPFVAEVPEGLPIGSSVFRVQVQDPDEAANGAVTMALQMGMPRLDFRLNITTGILVSTVVLDREQIGQYYLRIIAYDAGQFPRTSTSTLTITVLDVNDETPTFFPSVYNVSLEESVPRDYLVVRLNCTDNDAGLNAELSYFITAGNQDGKFSVGFRTGVVRTVVGLDRETQASYRLVIEAIDNGPAGGRRTGTATVYVEVLDVNDNRPIFLQNSYETSVLENIPRGTSILQVQATDADQGENGKVLYRILSGNNGQFSMDSLSGLISRGSVALDRETRSSNVLEVEAYNSDLGSMRSTVRVIVYVEDVNDEFPVFTQSQYNRLGLRETAGIGTSVVVVRATDPDTGDGGAVAYSLVSGSDRKFEVDVSTGLLTTVDYLDYETKTSYLMNVSATDQSPPFHRSYCTVYVTLLNELDEAVAFLSAGYEVSLRENIATGTEVVQVKAQSADNLNQLSYRFDPDTSPAALALFKIDSVTGRITVTGLLDRERGDSYTLTVVADDGGPKRGSTVKVSITILDENDNSPEFDITSDLSVNIPEDTAIGKRVAVVLARDKDAGRNGLVNFTLIAGNMQDVFKINTVNNTYGEVYLNAPLDRESVDRYLLKVRAMDNGSPPRYTDVSLTVNIVDVNDNPPVLQSPRGYNVSVSENVGGGTSVLRVVATDRDIGANAMLSYYITAGNQDLTFRMDRMTGEIVTRPSPPDREKQQQYTLTVTVEDDGTPTLSTSTTVWVRIIDENDNAPEFPEEEYVTELREGPNTVGAIIATVTAIDPDEGLNGTIRYAISKGNLAQTFQINSVTGQIVVVSALDYEISNGHYVLTVTATDQCPIPLLRLTSSTTVLVNVIDVNDNPPIFPRPFEGPFEITEGQPGPRVWTVKATDADSGSNGKVEYSITAGDLKNEFVISSVEGELRVRKDIELDRETIAYYNITITAKDLGTPSLNATVVVGILVLDINDNDPILLNLPANTSVSEGAAVQTFIIQVRAQDSDSGRNALLTYSITAGNSDGAFYINETSGVVQVNRPLDRERVAEYSLTITVKDNPDNPRIARRDSELLTVTLLDVNDNRPIFSQSSYRGEISENSPTGTTVLILNGPVLAEDKDIGVNAVVSYGLLGTRVDIFSIDSRTGVVSVRGGAVLDREAFSVPRVELFLVSVDVGGLNSSVPLTITILDVNDNPPVFNPSSFTVRLPENSPTGVVVTQLSATDADAGSNGWLQYRLDSGAQDRFIVDALSGAVLVGNATLDREERSSYRLVVIATDRGTPSLSGTATLTVVLDDVNDSRPRFIQPVQTISVNESTPPGVVVATLAAEDPDLKPRLEYYIISVEAKDDGNNAVDGLQQSFGIDFHTGAVFVRNPLNRELVATFEIIVSVHDNASDVIEMSVSVPNAKLTINVLDVNDNAPRFRPFGVANFSESILEGAVPGTTLLSVTAVDPDKGPNGQIIYQLLNLPRGNYIRLEDPSTGKIVANRTVDFEQVQWLNFTVRAQDHGSPPRFTELPVYLRIIDVNDNNPVFQQPLYQKSIFEDVALGTIIVSVRATDADSGLFSVIEYGLVDGEGKFGITPTTGDIYILSALDRETKDRYTLTAYARDNPGGSPNNRRENSVQVQLTVLDVNDFRPRFSERVFTTSVFENEPVGTSVITMKATDLDEGENALLTYSLQGPGADAFSLDPDTGLIRSLRLLQSFERFNLTVVATDQGRPPLWGTASLQITVIDVNDNRPIFVRPANGTIMHILEEQPPGLVVYEVFATDEDEGVNGEVRYSFLQTGAGNRDWENFHIDAVTGVITTAVKLDREKQALYSLVVVAYDLGQPVPYETMQPLQVALSDIDDNEPVFLKPPRGSLPYQSLSVPEHSSPGTVVGNVTGAVDADEGSNAIVYYFIAGGDTGGNFALSVSGVLRVKKDLDREEIPVYSIIVKASSNRNWTPPKGQRSQRAQVLDPSRDPTLQEVRIFLEDINDQSPRFTKLEYTTGVAADAKVGSDLIRVQAIDNDIGNNSLVLYHILSIRYIKLHSNDSEEMGNVFIIGETDGIICTFDLFMAYAPGYFIVEVLARDLAGHSDVALVSIYILRDDQRVKIVINEIPERVRLFQEEFINLLSNITGAIVNTDDVQFHVDKKGRVNFAQTDVLIHVVNKQTNRILDVEKVIQMIDENKEQLRNLFRNYNILDVQPAVTARAPDDLTTLQMAIIILAVLLFLAAMLFILMNWYYRTVHKRKLKAVVAGSTGNQGLMDILDMPNTNKYTFEGANPVWLDPFCRNLELAAQAEHEDDLPEDLSDIADLWNSPARTHGTFGREPQATKPEDDRYLRAAIQEYDNIAKLGQIMREGPIKGSLLNVVLDDYLRLKKLFAARLVTKSTSQGDRSSVTELIQSDLDEDDEERIGGRGTLRFKHKLPIELRGPDGVHAVHGSTGTLLTSDLNSLPEDDQRALARSLEALHADGGLYAERNARTESAKSTPLHRNKGSDTLSESPLEITEL b3djq7_danre myo7aa myo7a protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MVILQQGDYVWLDLKTGHEFEVPIGAVVKLCDSGQIQVLDDEGNEHWISPQNATNIKPMHPTSIHGVEDMIRLGDLNEAGILRNLLIRYREHLIYTYTGSILVAVNPYQLLPIYTADQIRLYTNKKIGEMPPHIFAIADNCYFNMQRNNKDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQARDERNYHIFYCMLKGMTPDQKKQLGLSKATDYTYLTIGNCTVCDGRDDQKEYSNIRSAMKVLMFTDKENWEISKLLAAILHMGNLRYEARIYDNLDACEVVRCSALTTAAVLLEVDLKDLMNCLTSRTIITRGETVSTPLSIEQALDVRDAFVKGIYGRLFVWIVEKINAAIYKPPSLELKAVRRSIGLLDIFGFENFMVNSFEQLCINFANENLQQFFVRHVFKLEQEEYNLENINWQHIEFTDNQDALDMIAIKPMNIISLIDEESKFPKGTDTTMLNKLNSQHKLNTNYIPPKNTYETQFGIQHFAGVVYYETRGFLEKNRDTLHGDIIQLVHSSKNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLSVCQPFFVRCIKPNEYKKPMLFDRELCVRQLRYSGMMETIRIRRAGYPIRYTFVEFVDRYRVLMPGVKPAYKQEDLRGTCQRIAEAVLGRDDDWQMGKTKIFLKDHHDMLLEIERDKAITDKVILIQKVVRGFKDRSNFLKMKKSAMLIQKTWRGYYCRKNYGAMRGGFSRLQALYRSRKLYQTYHVARQRIMLFQGRCRGFLVRRAFRHRLWAVITIQAYTRGMIARRLYKRLKGEYRRRLEAEKLRLAEEQKLRNQMSARKAKEEAEKMHQERLAQLAREDAEREKKERQEARRKMEMLDQMEKARQEPVNDSDMVDKMFGFLGTTNSFPGQEGQAPAGFEDLERTHRELEVEDLDESLPLPEDDLEDLSEYKFAKFSATYFQGTSTHTYMRRPLKQPLLFHEDEGDQLAALAVWITVLRFMGDLPEPKYHTAISDGSEKIPVMTKIYETLGKKTYKRELQALQGEGENTHIESHKKSSVRHKLVSLTLKKKSKITEEVTKRLNDGEYTVHGNSMLEDRPTSNLEKLHFIIGNGILRPGLRDEIYCQICKQLNQNPSKSSHARGWILMSLCVGCFAPSEKFVKYLRNFISEGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALSDKISLQDRFGFSLYIALFDKVSSLGSGNDHVMDAVSQCEQYAKEQGAQERNAPWRLFFRKEIFTPWHDPAEDAVATNLIYQQIVRGVKFGEYRCDREDLAELASQQYYVDYGSEILVERLLSLIPSYIPDREISSAKTVERWAQFIMAAHKKGVYTQKRVDPQKVKEEVVDFARYKWPLLFSRFYEAFKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEITAVSSSKGGKLQAQSFTLATIKADEFTFTSNNAEDIRDLVVTFLEGLRKRSKFVVALQDNPSPAADDSTFLSFLKGDLIVLDQDTGEQVMTSGWAHGTNDRTKQRGDFPADCVYVLPTVVRPPHDVVAFVTMTPDQRQESLRISHVPALTETEERVKPYTLEEFSYDYFRPPPKHTLSRVMITKNRGKDKLWCCTREPIKQALLKKCGGHEELSQEACMAFIAVMKYMGDYPSKRTRSVNELTDQIFEGALKAEPLKDEIYCQILKQLTENHIKYSEEKGWELLWLCVGLFPPSNVLLPHVQRFLQSKKHHPLALDCMQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCLNISSRLLLKTPEGFSLFVKISDKVISVPEGDFFFDFVRHLTDWIKKARPAKDGIVPSLTYQVFFMKKLWTTTVPGKDSFADSIFHYYQELPKYLRGYHKCSREEVFQLGALIYRVKFEDDKSHFPSIPKMLKEMIPQDLIRQLSPDDWKRSIVAYFNRHAGKSREEAKLMFLKIIFKWPTFGSAFFEVKQTTEPHFPEILLIAINKYGVSLIDPKNKDILTTYPFTKISNWSSGNTYFHITIGNLVQGSKLLCETSLGYKMDDLLTSYISQMLTTMSKQRNSRGGSSK q7t2e1_danre GATOR complex protein SEC13 Protein SEC13 homolog sec13 sec13l1 DKEY-72G22.2-001 protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MVSVINTVDTSHEDMIHDAQMDYYGTRLATCSSDRSVKIFDVKNGGQILVADLRGHEGPVWQVAWAHPMYGNILASCSYDRKVIIWKEENSTWDKMYEYTGHDSSVNSVCWGPYDFGLILACGSSDGAISVLTCSGDGHWDIKKINNAHTIGCNAVSWAPAVVPGSLIEQPTGQKPNYIKRFVSGGCDNLVKLWKEEDGQWKEDQKLEAHSDWVRDVGWAPSIGLPTSTIASCSQDGRVFIWTCDDPAGNTWTAKLLHKFNDVVWHVSWSITGNILAVSGGDNKVTLWKESVDGQWACISDVNKGQGAVSSITDSQQSEQ ift88-ush1c-4 1763494 n/a 1763499 unspecified role unspecified role neutral component neutral component n/a 1763500 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3C,I Labeling was greatly reduced or lost when any one protein was not expressed; the exception being for the mutant not expressing cdh23, as some signal remained for Cdh23-Harmonin,Cdh23-Ift88 and Harmonin-Ift88 protein pairs. cdh23-ush1c-2 1763494 n/a 1763501 unspecified role unspecified role neutral component neutral component n/a 1763500 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3A,G, Supp 7 Labeling was greatly reduced or lost when any one protein was not expressed; the exception being for the mutant not expressing cdh23, as some signal remained for Cdh23-Harmonin,Cdh23-Ift88 and Harmonin-Ift88 protein pairs. ift88-ush1c-4 1763494 n/a 1763500 unspecified role unspecified role neutral component neutral component n/a 1763502 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3E cdh23-ush1c-3 1763494 n/a 1763501 unspecified role unspecified role neutral component neutral component n/a 1763499 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3B, H Labeling was greatly reduced or lost when any one protein was not expressed; the exception being for the mutant not expressing cdh23, as some signal remained for Cdh23-Harmonin,Cdh23-Ift88 and Harmonin-Ift88 protein pairs. myo7aa-ush1c 1763494 n/a 1763502 unspecified role unspecified role neutral component neutral component n/a 1763501 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3D cdh23-myo7aa 1763494 n/a 1763502 unspecified role unspecified role neutral component neutral component n/a 1763499 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3F ift88-myo7aa 1763494 n/a 1763499 unspecified role unspecified role neutral component neutral component n/a 1763503 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 5C,M,W The interaction was much reduced in cells not expressing ift88 or cdh23 cdh23-ush1c-1 1763495 n/a 1763501 unspecified role unspecified role neutral component neutral component n/a 1763500 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1G, S2 ift88-ush1c-2 1763495 n/a 1763499 unspecified role unspecified role neutral component neutral component n/a 1763500 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1F, S2 ift88-ush1c-3 1763495 n/a 1763502 unspecified role unspecified role neutral component neutral component n/a 1763500 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1H, S2 cdh23-ush1c-4 1763496 n/a 1763500 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1763501 unspecified role unspecified role bait bait intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false syncam2_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 4A The intracellular Cdh23 domain, without membrane tethering (the syncam2 tag), did not co-precipitate with Harmonin. (The synCAM2 tag is made up from the signal peptide from human CD33 joined with the extracellular domain of human IGSF4D (Ala 21-Asp 335) fused with a 6X histidine tag at the C-terminus.) cdh23-ush1c-6 1763497 n/a 1763500 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1763501 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range false syncam2_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 4B The intracellular Cdh23 domain, without membrane tethering (the syncam2 tag), did not co-precipitate with Harmonin. (The synCAM2 tag is made up from the signal peptide from human CD33 joined with the extracellular domain of human IGSF4D (Ala 21-Asp 335) fused with a 6X histidine tag at the C-terminus.) cdh23-ush1c-9 1763498 n/a 1763500 unspecified role unspecified role neutral component neutral component c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1763501 unspecified role unspecified role neutral component neutral component syncam2_tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false intracellular_domain sufficient to bind sufficient binding region sufficient to bind range range range range false colocalization colocalization false false false Supp 10A The synCAM2 tag is made up from the signal peptide from human CD33 joined with the extracellular domain of human IGSF4D (Ala 21-Asp 335) fused with a 6X histidine tag at the C-terminus. It functions as a membrane tether for the cdh23 intracellular domain.