IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ karag_z-2014-2 Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Cell (0092-8674) 2014 Only protein-protein interactions imex curation Alzheimers - Interactions investigated in the context of Alzheimers disease karag_z-2014-1 Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Cell (0092-8674) 2014 Only protein-protein interactions imex curation Alzheimers - Interactions investigated in the context of Alzheimers disease karag_z-2014-3 Hsp90-Tau complex reveals molecular basis for specificity in chaperone action. in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Karagöz G.E., Duarte A.M., Akoury E., Ippel H., Biernat J., Morán Luengo T., Radli M., Didenko T., Nordhues B.A., Veprintsev D.B., Dickey C.A., Mandelkow E., Zweckstetter M., Boelens R., Madl T., Rüdiger S.G. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. t.madl@tum.de, s.g.d.rudiger@uu.nl Cell (0092-8674) 2014 Only protein-protein interactions imex curation Alzheimers - Interactions investigated in the context of Alzheimers disease hs90b_human Heat shock protein HSP 90-beta HSP90AB1 HSP90B HSPC2 HSPCB Heat shock 84 kDa protein protein human Homo sapiens Human MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hsp90ab1-mapt-1 1537226 n/a 1537229 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1537230 unspecified role unspecified role neutral component neutral component region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1B, 1D hsp90ab1-mapt-2 1537226 n/a 1537230 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1537229 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false isoleucine-labelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig. 2, Supp. Fig. 1 adenosine_5___gamma_thio_triphosphate-hsp90ab1 1537226 n/a 1537231 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1537229 unspecified role unspecified role neutral component neutral component isoleucine-labelled 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1537230 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2, Supp. Fig. 1 hsp90ab1-mapt 1537227 n/a 1537229 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1537230 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1537227 Fig. 1A The affinity of Hsp90’s for Tau in the ATP state did not differ significantly from the affinity of Hsp90 in the nucleotide-free apo state. hsp90ab1-mapt-3 1537228 n/a 1537229 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1537230 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3