MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014p-1 Mcp1p tracks microtubule plus ends to destabilize microtubules at cell tips schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence chuanhai@hku.hk 2014 imex curation Zheng F., Li T., Cheung M., Syrovatkina V., Fu C. FEBS Lett. (0014-5793) Only protein-protein interactions tba2_schpo Tubulin alpha-2 chain tub1 alp2 atb2 SPBC800.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMEVDYMEEEY mcp1_schpo Meiotic coiled-coil protein 1 mcp1 SPAC1687.10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MELAKIGDRLNNYYNNIYTLSEKLGYSFTKISFNNIILDALSGHQRKLEIKIINIESKCNELSSEISLLRKRLGIIEEIEIPRTLIDQYECLVTYHNGIRAIYNTKKNEAKNMYKEIERLSVILGESVDEVNFVNGDFEDISDIKLEQLSAKLNELHQIYRLRKEKNIEIKEELKKLQDSLSIKLSMPADDLSKNGIKHLIIYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPTSELPTNYSDKTLTLWENELSSMRKVYFVKYKTEYEFLQKRYLSLARIMFVSKKEMLFTSQFKEIPNVNRELIQSMKQEIGNLEVDLHLQGELNNMIRRYLLLESRVYKESQRMKVLSGSASQPFTLKRLQKDFQLLKAKLICALREWEEDNEKKIYVFGKPLSSRLLMIHSPPILQNQENISSNDNSKSSPESSPPARKTTGKIENKKLRNIDVQSKKSIRFPLRLASHEEPLIDRMVNQSPDTRSVSKSKRLVRETSSGCLFKNHSKSNTLSPRRKGSRHGPREPCLSPDASSSSIPVTDIKEILPSQHDTPHNSVKLTGSSTTPASVSLRQMIEPLLSRTPPKGEFTNSLDDTPTQSNVEKVDRFLENHDWETCSESSSASEDQSLCKQLSSFSLSSSDISKKLKNNLNGLHFSSDEGSKLPTFL mal3_schpo Microtubule integrity protein mal3 mal3 SPAC18G6.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSESRQELLAWINQVTSLGLTRIEDCGKGYAMIQIFDSIYQDIPLKKVNFECNNEYQYINNWKVLQQVFLKKGIDKVVDPERLSRCKMQDNLEFVQWAKRFWDQYYPGGDYDALARRGNRGPANTRVMNSSAGATGPSRRRQVSSGSSTPSMTKSSANNNNVSSTANTAAVLRAKQAQQQITSLETQLYEVNETMFGLERERDFYFNKLREIEILVQTHLTTSPMSMENMLERIQAILYSTEDGFELPPDQPADLTTALTDHDTNNVAEEAQMTDLKDSETQRVPSAPDFVHARLQSLEVDDDENITF 9527D31CAA024256 mcp1-tub1 1479296 n/a atb2p 1479297 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a mcp1p 1479298 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell yfp tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f1b In our microscopy screen for new MAPs, we found that mcp1p displayed remarkable staining along 3 cytoplasmic microtubules when overexpressed from the inducible nmt promoter in vegetative cells (Fig. 1B). This microtubule localization is consistent with the result from the ORFeome localization project [20]. In addition, we noted that overexpressed mcp1p appeared to concentrate near microtubule plus ends, adjacent to cell tips. Consistent with this, the endogenous mcp1p tagged with three tandem GFPs at its carboxyl-terminus (mcp1p-3GFP) mainly resided at a microtubule region near cell tips, likely at microtubule plus ends (Fig 1C). These findings suggest that mcp1p is also a MAP in vegetative cells. mcp1-tub1-1 1479296 n/a atb2p 1479297 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a mcp1p 1479298 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f1c f3b sf2b In our microscopy screen for new MAPs, we found that mcp1p displayed remarkable staining along 3 cytoplasmic microtubules when overexpressed from the inducible nmt promoter in vegetative cells (Fig. 1B). This microtubule localization is consistent with the result from the ORFeome localization project [20]. In addition, we noted that overexpressed mcp1p appeared to concentrate near microtubule plus ends, adjacent to cell tips. Consistent with this, the endogenous mcp1p tagged with three tandem GFPs at its carboxyl-terminus (mcp1p-3GFP) mainly resided at a microtubule region near cell tips, likely at microtubule plus ends (Fig 1C). These findings suggest that mcp1p is also a MAP in vegetative cells. mcp1-tub1-1 1479296 n/a mal3p 1479299 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a mcp1p 1479298 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f1c f3b sf2b In our microscopy screen for new MAPs, we found that mcp1p displayed remarkable staining along 3 cytoplasmic microtubules when overexpressed from the inducible nmt promoter in vegetative cells (Fig. 1B). This microtubule localization is consistent with the result from the ORFeome localization project [20]. In addition, we noted that overexpressed mcp1p appeared to concentrate near microtubule plus ends, adjacent to cell tips. Consistent with this, the endogenous mcp1p tagged with three tandem GFPs at its carboxyl-terminus (mcp1p-3GFP) mainly resided at a microtubule region near cell tips, likely at microtubule plus ends (Fig 1C). These findings suggest that mcp1p is also a MAP in vegetative cells.