MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014s-2 Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities in vitro In vitro protease assay protease assay weight by staining molecular weight estimation by staining weight by staining evakutej@hotmail.com; jacob.bauer@savba.sk 2014 imex curation Ambro L., Pevala V., Ondrovičová G., Bellová J., Kunová N., Kutejová E., Bauer J. FEBS J. (1742-464X) Only protein-protein interactions anonymous-2014s-1 Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie evakutej@hotmail.com; jacob.bauer@savba.sk 2014 imex curation Ambro L., Pevala V., Ondrovičová G., Bellová J., Kunová N., Kutejová E., Bauer J. FEBS J. (1742-464X) Only protein-protein interactions casb_bovin Beta-casein CSN2 protein protein bovin Bos taurus (Bovine) Bovine MKVLILACLVALALARELEELNVPGEIVESLSSSEESITRINKKIEKFQSEEQQQTEDELQDKIHPFAQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFLQPEVMGVSKVKEAMAPKHKEMPFPKYPVEPFTESQSLTLTDVENLHLPLPLLQSWMHQPHQPLPPTVMFPPQSVLSLSQSKVLPVPQKAVPYPQRDMPIQAFLLYQEPVLGPVRGPFPIIV lonm_human Lon protease homolog, mitochondrial LONHs PRSS15 LONP1 Lon protease-like protein Mitochondrial ATP-dependent protease Lon Serine protease 15 protein protein human Homo sapiens Human MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER csn2-lonp1 1480515 n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Gly894Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-1 1480515 n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893_Gly894delinsAlaPro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-2 1480515 n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Trp770Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-3 1480515 n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cleavage cleavage reaction cleavage false false false f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-4 1480515 n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893_Gly894delinsProAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cleavage cleavage reaction cleavage false false false f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-5 1480515 n/a hLonP 1480518 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified P36776:p.Lys529Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a beta casein 1480517 weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cleavage cleavage reaction cleavage false false false f3 t1 sf23 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) lonp1-lonp1 1480516 n/a hLon 1480518 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a hLon 1480518 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false sf13 All mutants showed the same pattern of oligomerization as wild-type hLon by both gel-filtration and glutaraldehyde cross-linking (supplementary Figure S13). These residues, therefore, do not appear to be important for proper oligomerization. Correct oligomerization seems to be important for the proper functioning of Lon’s various activities, so verification that all mutants have the same oligomeric form as the wild-type allows us to directly compare their various activities and to look for the source of their differences only in their local effects