MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint brami_cherrier-2014-5 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-7 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-11 FAK dimerization controls its kinase-dependent functions at focal adhesions. mouse-fibroblast Mus musculus fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag visualisation tag visualisation Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-1 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-2 FAK dimerization controls its kinase-dependent functions at focal adhesions. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-3 FAK dimerization controls its kinase-dependent functions at focal adhesions. mouse-fibroblast Mus musculus fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-4 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-6 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-8 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-9 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-10 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-12 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro protein kinase assay protein kinase assay antibody detection identification by antibody antibody detection Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation brami_cherrier-2014-13 FAK dimerization controls its kinase-dependent functions at focal adhesions. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Brami-Cherrier K., Gervasi N., Arsenieva D., Walkiewicz K., Boutterin M.C., Ortega A., Leonard P.G., Seantier B., Gasmi L., Bouceba T., Kadaré G., Girault J.A., Arold S.T. jean-antoine.girault@inserm.fr; stefan.arold@kaust.edu.sa jean-antoine.girault@inserm.fr,stefan.arold@kaust.edu.sa EMBO J. (0261-4189) 2014 Only protein-protein interactions imex curation fak1_rat Focal adhesion kinase 1 Ptk2 Fak Fak1 Focal adhesion kinase-related nonkinase Protein-tyrosine kinase 2 p125FAK pp125FAK protein protein rat Rattus norvegicus (Rat) Rat MAAAYLDPNLNHTPSSSTKTHLGTGTERSPGAMERVLKVFHYFESSNEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQDIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFNQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGATQSFIIRPQKEGERALPSIPKLANNEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGVYLSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKVQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQISGYPGSHGIPAMAGSIYPGQASLLDQTELWNHRPQEMSMWQPSVEDSAALDLRGMGQVLPPHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSFQGPTGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLSNLSSISSPAESYNEGVKPWRLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPILPASTHREIEMAQKLLNSDLGELISKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH paxi_mouse Paxillin Pxn protein protein mouse Mus musculus Mouse MDDLDALLADLESTTSHISKRPVFLSEEPPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTVLDPLDQWQPSGSRYAHQQPPSPLPVYSSSAKNSSASNTQDGVGSLCSRAGEEEHVYSFPNKQKSAEPSPTVMSSSLGSNLSELDRLLLELNAVQHSPPGFPADEAESSPPLPGALSPLYGIPENNTPLGGKAGPLVKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSSQQQTRISASSATRELDELMASLSDFKMQGLEQRVDGERPWAAGWPPSSRQSSPEGQDEGGFMAQGKTGSSSPPGGLSKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHSLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQSCFVKLFC fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH ptk2-ptk2-7 1512865 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false sf2a ptk2-ptk2-11 1512865 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified O35346:p.[Lys216Ala;Lys218Ala;Arg221Ala;Lys222Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false sf4a ptk2-3 1512866 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O35346:p.Trp266Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false f4c f4d f4e sf4c ptk2-ptk2-13 1512866 n/a 1512878 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false sf4c-e ptk2-pxn-13 1512867 n/a 1512878 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed fixed cell fixed cell mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 1512879 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false sf1c f3d ptk2-pxn-14 1512867 n/a 1512878 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed fixed cell fixed cell O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1512878 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed fixed cell fixed cell O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false mcherry tag mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false proximity proximity close-contact colocalization neighbourhood interaction false false false f3a f3b f3c ptk2-3 1512868 n/a 1512880 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false f1 st1 ptk2-ptk2-5 1512869 n/a 1512878 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1512878 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false vsv tag vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f2a ptk2-ptk2-6 1512870 n/a 1512879 immunostaining immunostaining unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell n/a 1512878 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false f2c f2d In HA-FAK-transfected cells, confocal analysis revealed that HA immunoreactivity was co-localized with paxillin at FAs (Fig 2C, upper panels), as expected. HA-W266A-FAK was also detected at FAs, but a larger proportion was cytosolic than for the wild-type (WT), as predicted by our model (Fig 2C and D, lower panels). ptk2-ptk2-12 1512871 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1512871 sf4b ptk2-3 1512872 n/a 1512878 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false f5a f5b sf3 ptk2-3 1512873 n/a 1512878 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified O35346:p.Trp266Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false f4a f4b ptk2-ptk2-8 1512874 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1512874 sf2a ptk2-ptk2-9 1512875 n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1512878 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O35346:p.[Lys216Ala;Lys218Ala;Arg221Ala;Lys222Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f5c f5d sf4g ptk2-3 1512876 n/a 1512878 self self self self purified purified O35346:p.Trp266Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false phosphorylation phosphorylation reaction phosphorylation false true false f6a ptk2-3 1512877 n/a 1512878 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified O35346:p.Trp266Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false f4f