MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint das-2013a-2 Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine. in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Das R., Liang Y.H., Mariano J., Li J., Huang T., King A., Tarasov S.G., Weissman A.M., Ji X., Byrd R.A. byrdra@mail.nih.gov EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation das-2013a-1 Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Das R., Liang Y.H., Mariano J., Li J., Huang T., King A., Tarasov S.G., Weissman A.M., Ji X., Byrd R.A. byrdra@mail.nih.gov EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation das-2013a-3 Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine. in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Das R., Liang Y.H., Mariano J., Li J., Huang T., King A., Tarasov S.G., Weissman A.M., Ji X., Byrd R.A. byrdra@mail.nih.gov EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation ub2g2_human Ubiquitin-conjugating enzyme E2 G2 Ubiquitin-protein ligase G2 Ubiquitin carrier protein G2 UBE2G2 E2 ubiquitin-conjugating enzyme G2 UBC7 protein protein human Homo sapiens Human MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL amfr_human E3 ubiquitin-protein ligase AMFR gp78 RING finger protein 45 AMFR RNF45 Autocrine motility factor receptor protein protein human Homo sapiens Human MPLLFLERFPWPSLRTYTGLSGLALLGTIISAYRALSQPEAGPGEPDQLTASLQPEPPAPARPSAGGPRARDVAQYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLVAMLLSCCGLAAVCSITGYTHGMHTLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRVREEHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSDSIRPALNSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKSSEDDAASESFLPSEGASSDPVTLRRRMLAAAAERRLQKQQTS amfr-amfr-1 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 st1 f2a f2b sf1b amfr-amfr-2 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.[Ser111Asn;Val113Thr] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1766312 t1 amfr-amfr-3 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.[Ser111Asn;Val113Thr] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-ube2g2-3 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction false false false 1766312 st1 amfr-ube2g2-4 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Trp368Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1766312 t1 amfr-ube2g2-5 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Thr377Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1766312 t1 amfr-amfr-5 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Thr377Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-amfr-6 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q9UKV5:p.Trp368Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false 1766312 t1 amfr-amfr-7 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.Glu108Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-amfr-8 1766312 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Arg379Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-amfr-9 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.Glu108Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q9UKV5:p.Arg379Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-amfr-10 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.Glu108Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q9UKV5:p.Arg379Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-ube2g2-6 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Q9UKV5:p.Arg379Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-ube2g2-7 1766312 n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P60604:p.Glu108Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1766312 t1 amfr-ube2g2 1766313 n/a 1766316 peptide synthesis peptide synthesis unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified direct interaction direct interaction false false false 1766313 f1b t1 amfr-ube2g2-1 1766313 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified direct interaction direct interaction false false false 1766313 f1c t1 amfr-ube2g2-2 1766313 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified ubiquitinylated lysine ubiquitinylated lysine ubiquitinylated lysine predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1766313 t1 amfr-amfr-11 1766314 n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766316 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 1766315 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false f2c