IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ happel-2011-1 Opioid-induced chemokine expression requires NF-κB activity: the role of PKCζ. human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Happel C., Kutzler M., Rogers T.J. IBD - Inflammatory bowel disease In Fig. 1, the authors show increased binding of PBMC nuclear extract to NF-kB and TFIID DNA consensus sequences upon treatment with DAMGO (CHEBI:272), as determined by TranSignal protein/DNA Array I (Panomics); however, it was not possible to establish the sequence of the consensus oligonucleotides or the exact identity of the nuclear proteins that bound to these probes, hence this experiment is not captured here. rogerst@temple.edu J. Leukoc. Biol. (0741-5400) 2011 Only protein-protein interactions imex curation tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS ccl2_human_gene C-C motif chemokine 2 CCL2 MCP1 SCYA2 HC11 Monocyte chemoattractant protein 1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte secretory protein JE Small-inducible cytokine A2 gene gene human Homo sapiens Human rela-ccl2-3 1768049 n/a 1768050 unspecified role unspecified role bait bait n/a 1768051 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 6 (D-Ala(2)-mephe(4)-gly-ol(5))enkephalin (DAMGO) (CHEBI:272) CHEBI:272 increases binding of Q04206 to ENSG00000108691 CHEBI:272 indirectly increases expression at RNA level of ENSG00000108691 CHEBI:272 indirectly increases expression at protein level of ENSG00000108691