UniProt UniProt http://www.uniprot.org kumar-2010a-1 Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Genes Dev. (0890-9369) 2010 mimix curation kumar-2010a-2 Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Genes Dev. (0890-9369) 2010 mimix curation kumar-2010a-3 Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Genes Dev. (0890-9369) 2010 mimix curation re114_mouse Meiotic recombination protein REC114 Rec114 protein protein mouse Mus musculus Mouse MSEAGNVASGLGLPGEVSQWSLKRYGRFMLLDNVGSPGPSSEAAAAGSPTWKVFESSEESGSLVLTIVVSGHFFISQGQTLLEGFSLIGSKNWLKIVRRMDCLLFGTTIKNKSRMFRVQFSGESKEEALERCCGCVQTLAQYVTVQEPDSTTQELQQSQGPREAGESQGKDPLQQGPSLTLEQHVCMAAGAGVLQERTSVTHRAQSILAPEKLTLAYEGSSWGTEELGPFLRLCLMDQNFPAFVEEVEKELKKITGLRN mei4_mouse Meiosis-specific protein MEI4 Mei4 protein protein mouse Mus musculus Mouse MDIQPWYLKTSKLALALAIIHSKPADRSSREYTEYLASLVTQKESTWKSKLEALEAEVLQLRQKLLLSRISSGLFKNGPDVLPTLSDQEPTSSENTLTLMDDSGCVLSNEQRNEPAELSQHFVESTDPPLLPLPLEKRPRTTLENPLSSHMQFFQHLLELKKWTESSSLKVYLTHFEKDSSTVSDSVSQLLDALITFYRNPKLPFSSFWTEAVGTLARLASDFNLSNHIFKRCSKKLEEFEKTLLQAILENNSINRFQVQRYVSQSLVTLGSCSLLRKSIISLLLSEVNSFVDDLGAIDQDQGIYDVTRYENIFSLFWILEQVLQQAPQGDRTAHMDHSIPEMQTFLQKHDEVIFRLSDAFPLFAFYLWRLGVLLNSAEMETVKNESLP mei4-r114l-2 1540365 n/a 1540368 unspecified role unspecified role prey prey n/a 1540369 unspecified role unspecified role bait bait physical association physical association false false false 3A bottom r114l-mei4-2 1540365 n/a 1540368 unspecified role unspecified role bait bait n/a 1540369 unspecified role unspecified role prey prey physical association physical association false false false S5A r114l-mei4-1 1540366 n/a 1540368 unspecified role unspecified role bait bait n/a 1540369 unspecified role unspecified role prey prey physical association physical association false false false 3B and S6A mei4-r114l-1 1540366 n/a 1540368 unspecified role unspecified role prey prey n/a 1540369 unspecified role unspecified role bait bait physical association physical association false false false 3B and S6B mei4-r114l-4 1540367 n/a 1540368 unspecified role unspecified role prey prey n/a 1540369 unspecified role unspecified role bait bait direct interaction direct interaction false false false S5B