HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html mohd_ismail-2009-1 The hepatitis C virus core protein contains a BH3 domain that regulates apoptosis through specific interaction with human Mcl-1. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mohd-Ismail N.K., Deng L., Sukumaran S.K., Yu V.C., Hotta H., Tan Y.J. Virus - Publications including interactions involving viral proteins The virus is stated to be strain 1B, cloned in Singapore. However his strain cannot be found in either UniProt or NCBI taxonomy. UniProt entry Q91AU0 has been used for the Viral Polyprotein, as this is the only UniProt entry with !00% homology to the sequence shown in figure 1A. mcbtanyj@imcb.a-star.edu.sg J. Virol. (0022-538X) 2009 imex curation Only protein-protein interactions mohd_ismail-2009-2 The hepatitis C virus core protein contains a BH3 domain that regulates apoptosis through specific interaction with human Mcl-1. human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Mohd-Ismail N.K., Deng L., Sukumaran S.K., Yu V.C., Hotta H., Tan Y.J. Virus - Publications including interactions involving viral proteins The virus is stated to be strain 1B, cloned in Singapore. However his strain cannot be found in either UniProt or NCBI taxonomy. UniProt entry Q91AU0 has been used for the Viral Polyprotein, as this is the only UniProt entry with !00% homology to the sequence shown in figure 1A. mcbtanyj@imcb.a-star.edu.sg J. Virol. (0022-538X) 2009 imex curation Only protein-protein interactions mohd_ismail-2009-3 The hepatitis C virus core protein contains a BH3 domain that regulates apoptosis through specific interaction with human Mcl-1. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mohd-Ismail N.K., Deng L., Sukumaran S.K., Yu V.C., Hotta H., Tan Y.J. Virus - Publications including interactions involving viral proteins The virus is stated to be strain 1B, cloned in Singapore. However his strain cannot be found in either UniProt or NCBI taxonomy. UniProt entry Q91AU0 has been used for the Viral Polyprotein, as this is the only UniProt entry with !00% homology to the sequence shown in figure 1A. mcbtanyj@imcb.a-star.edu.sg J. Virol. (0022-538X) 2009 imex curation Only protein-protein interactions q91au0_9hepc Core protein precursor Mature core protein NS3 helicase NS3 protease Protease NS2 Serine protease/helicase NS3 Viroporin p7 Viroporin p70 p23 p56/58 Envelope glycoprotein E1 Envelope glycoprotein E2 Genome polyprotein Gp32 Hepacivirin NS1 NS3P NS5B Non-structural protein 4A Non-structural protein 4B Non-structural protein 5A RNA-directed RNA polymerase gp35 gp68 gp70 p21 p27 p68 p8 protein protein hepatitis c virus subtype 1b Hepatitis C virus subtype 1b MSTIPKPQRKTKRNTYRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRPEGWAWAQPGYPWPLYGNEGLGWAGWLLSPRGSRPSWGPIDPRRRSRNLGKVIDTLTCGFADLMGYIPLVGAPLGGAARALAHGVRVLEDGVNYATGNLPGCSFSIFLLALLSCLTIPASAYEVRNASGVYHVTNDCSNSSIVYEAADIIMHTPGCVPCVRENNSSRCWVALTPTLAARNLSVPTTTIRRHVDLLVGAAAFCSAMYVGDLCGSVLLVSQLFTLSPRQHETVQDCNCSLYPGHVTGHRMAWDMMMNWSPTTALVLSQILRIPQTIVDMVAGAHWGVLAGIAYYSMVGNWAKVLVVMLLFAGVDGHTQVMGGSQASTINTLTGIFSPGAKQKIQLINTNGSWHINRTALNCNDSLNTGFLAALFYTHSFNSSGCLERMASCRPIDKFDQGWGPITYDEGPDLDQRPYCWHYAPRSCGIVPASQVCGPVYCFTPSPVVVGTTDRTGAPTYRWGENETDVLILNNTRPPQGNWFGCTWMNSTGFTKTCGGPPCNIGGAGNNTLVCPTDCFRKHPEATYTKCGSGPWLTPRCMVDYPYRPWHYPCTVNFTIFKVRMYVGGVEHRLNAARNWTRGERCDLEDRDRSELSPLLLSTTEWQILPCSFTTLPALSTGLIHLHQNIVDVQYLYGIGSVVVSLVIKWEYVLLSFFLLADARVCACLWMMLLIAQAEAALENLVVLNAASVAGAHGILTFLVFFCAAWYIKGRLVPGAAYAFYGVWPLLLLLLALPPRAYAMDREMAASCGGAVFIGLVLLTLSPHYKVFLARLIWWLQYFTTRAEAILHVWVPPLNVRGGRDAVILLTCAVHPDLIFDITKLLLAVLGPLMVFLAGITRVPYFVRAQGLIRACALARKVAGGHYIQMALMKLAALTGTYLYDHLTPLRDWAHAGLRDLAVAVEPVVFSDMETKIITWGADTAACGDIILGLPVSARRGREILLGPADSLEGQGWRLLAPITAYSQQTRGLLGCIITSLTGRDKNQVEGEVQVVSTATQSFLATCVNGVCWTVFHGAGSKTLAGPKGPITQMYTNVDQDLVGWQAPPGARSLTPCTCGSSDLYLVTRHADVIPVRRRGDNRGSLLSPRPVSYLKGSSGGPLLCPSGHAVGIFRAAVCTRGVAKAVDFIPVESMETTMRSPVFTDNSSPPAVPQTFQVAHLHAPTGSGKSTKVPAAYAAQGYKVLVLNPSVAATLGFGTYMSKAYGVDPNIRTGVRTITTGAPITYSTYGKFLADGGCSGGAYDIIMCDECHSTDSTTVLGIGTVLDQAETAGARLVVLATATPPGSVTVPHPNIEEIALSNTGEIPFYGKAIPIETIKGGRHLIFCHSKKKCDELAAKLSGLGLNAVAYYRGLDVSVIPTSGDVVVVATDALMTGFTGDSDSVIDCNTCVTQTVDFSLDPTFTIETTTVPQDAVSRSQRRGRTGRGRGGIYRFVTPGERPSGMFDSSVLCECYDAGCAWYELTPAETSVRLRAYLNTPGLPVCQDHLEFWESVFTGLTHIDAHFLSQTKQAGDNFPYLVAYQATVCARAQAPPPSWDQMWKSLIRLKPTLHGPTPLLYRLGTVQSEITLTHPVTKYIMACMSADLEVVTSTWVLVSGVLAALAAYCLTTGSVVIVGRIILSGKPAVIPDREVLYREFDEMEECASHLPYIEQGMQLAEQFKQKALGLLQTATKQAEAAAPVVESKWRALEAFWAKHMWNFISGIQYLAGLSTLHGNPAIASLMAFTASITSPLTTQHTLLFNILGGWVAAQLAPPSAASAFVGAGIAGAAVGSIGLGKVLVDVLAGYGAGVAGALVAFKVMSGEVPSTEDLVNLLPAVLSPGALVVGVVCAAILRRHVGPGEGAVQWMNRLIAFASRGNHVSPTHYVPESDAAARVTQILSSLTVTQLLRRLHQWINEDCSTPCSGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYRGVWRGDGIMQTTCPCGAQISGHVKNGSMRIVGPRSCSNTWHGTFPINAYTTGPCTPAPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFQVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLDRGSPPSLASSSASQLSAPSLKATCTTRHDSPDAGLIEANLLWRQEMGGNITRVESENKVVILDSFEPLRAEEDEREVSVPAEILRKSRKFPRAMPIWARPDYNPPLLESWKNPDYVPPVVHGCPLPPIKGPPIPPPRRKRTVVLTESTVSSALAELATKTFGSSGSSAVDSGTASAPPDQPSDNGDAGSDAESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCCSMSYTWTGALITPCAAEESKLPINALSNSLLRHHNMVYATTSRSASQRQKKVTFDRLQVLDDHYRDVLKEMKAKASTVKAKLLSVEEACKLTPPHSAKSKFGYGAKDVRNLSSKAVNHIRSVWKDLLEDTETPIDTTIMAKNEVFCVQPEKGGRKPARLIVFPDLGVRVCEKMALYDVVSTLPQAVMGSSYGFQYSPGQRVEFLVNAWKSKKSPMGFAYDTRCFDSTVTESDIRVEESIYQCCDLVPEARQAIRSLTERLYVGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQDCTMLVCGDDLVVICESAGTQEDAASLRVFTEAMTRYSAPPGDPPQPEYDLELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGAYYSIEPLDLPQIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRAWRHRARSVRAKLLSQGGRAATCGKYLFNWAVKTKLKLTPIPAASQLDLSGWFVAGYGGGDIYHSLSRARPRWFMLCLPLLSVGVGINLLPNR mcl1_human Induced myeloid leukemia cell differentiation protein Mcl-1 Bcl-2-related protein EAT/mcl1 mcl1/EAT MCL1 BCL2L3 Bcl-2-like protein 3 protein protein human Homo sapiens Human MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR p26660-pro_0000037609 Mature core protein protein protein hcvj6 Hepatitis C virus genotype 2a (isolate HC-J6) (HCV) STNPKPQRKTKRNTNRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKDRRSTGKSWGKPGYPWPLYGNEGLGWAGWLLSPRGSRPSWGPNDPRHRSRNVGKVIDTLTCGFADLMGYIPVVGAPLGGVARALAHGVRVLEDGVNFATGNLPGCSFSIF 2 177 core-mcl1 1547113 n/a 1547116 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q91AU0:p.Leu126Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q91AU0:p.Val122Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q91AU0:p.Leu119Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1547117 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3A and 7A core-mcl1-1 1547114 n/a 1547117 unspecified role unspecified role bait bait n/a 1547116 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B, Supp figure 1. mcl1-p26660_pro 1547115 n/a 1547117 unspecified role unspecified role bait bait n/a 1547118 unspecified role unspecified role prey prey P26660-PRO_0000037609:p.Gly119Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 9G