IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liu-2006o-1 A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. rat-rfpec Rattus norvegicus epididymal fat pad endothelial cells rat-rfpec rat epididymal fat pad endothelial cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liu M., Horowitz A. Synectin was identified as Gipc1 from reference Zhang et al., 2004. arie.horowitz@dartmouth.edu Mol. Biol. Cell (1059-1524) 2006 imex curation Only protein-protein interactions liu-2006o-2 A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. rat-rfpec Rattus norvegicus epididymal fat pad endothelial cells rat-rfpec rat epididymal fat pad endothelial cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Liu M., Horowitz A. Synectin was identified as Gipc1 from reference Zhang et al., 2004. arie.horowitz@dartmouth.edu Mol. Biol. Cell (1059-1524) 2006 imex curation Only protein-protein interactions liu-2006o-4 A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. rat-rfpec Rattus norvegicus epididymal fat pad endothelial cells rat-rfpec rat epididymal fat pad endothelial cells confocal microscopy confocal microscopy immunostaining immunostaining Liu M., Horowitz A. Synectin was identified as Gipc1 from reference Zhang et al., 2004. arie.horowitz@dartmouth.edu Mol. Biol. Cell (1059-1524) 2006 imex curation Only protein-protein interactions gipc1_rat PDZ domain-containing protein GIPC1 RGS19-interacting protein 1 GAIP C-terminus-interacting protein RGS-GAIP-interacting protein GLUT1 C-terminal-binding protein Gipc1 Gipc Rgs19ip1 Synectin protein protein rat Rattus norvegicus (Rat) Rat MPLGLGRRKKAPPLVENEEAEPSRSGLGVGEPGPLGGSGAGESQMGLPPPPASLRPRLVFHTQLAHGSPTGRIEGFTNVKELYGKIAEAFRLPAAEVMFCTLNTHKVDMDKLLGGQIGLEDFIFAHVKGQRKEVEVFKSEDALGLTITDNGAGYAFIKRIKEGSVIDHIQLISVGDMIEAINGQSLLGCRHYEVARLLKELPRGRTFTLKLTEPRKAFDMISQRSSGGHPGSGPQLGTGRGTLRLRSRGPATVEDLPSAFEEKAIEKVDDLLESYMGIRDTELAATMVELGKDKRNPDELAEALDERLGDFAFPDEFVFDVWGAIGDAKVGRY pkhg5_rat Pleckstrin homology domain-containing family G member 5 Plekhg5 Tech Neuronal RhoA GEF protein Transcript highly enriched in cortex and hippocampus protein protein rat Rattus norvegicus (Rat) Rat MDKGRAAKVCHHADCQQLHHRGPLNLCEICDSKFHNTTHYDGHVRFDLPPQGSVLARNVSTRSCPPRTSPAGDLEEEDEGYTNGKGDRKSAGLKISKKKARRRHTDDPSKECFTLKFDLNVDIETEIVPAMKKKSLGEVLLPVFERKGIALGKVDIYLDQSNTPLSLTFEAYRFGGHYLRVKAKPGDEGKVEQGVKDSKSLSLPALRPSGAGTPVLERVDPQSRRESSLDILAPGRRRKNMSEFLGDTSIPGQESPAPSSCSLPVGSSVGSSGSSESWKNRAASRFSGFFSSSPSTGAFGREVDKMEQLESKLHAYSLFGLPRMPRRLRFDHDSWEEEEDDEEEEDNSGLRLEDSWRELIDGHEKLTRRQCHQQEAVWELLHTEVSYIRKLRVITNLFLCCLLNLQESGLLCEVEAERLFSNIPELARLHRGLWSSVMVPVLEKARRTRALLQPSDFLKGFKMFGSLFKPYIRYCMEEEGCMEYMRSLLRDNDLFRAYVTWAEKHQQCQRLKLSDMLAKPHQRLTKYPLLLKSVLRKTDEPRAKEAIITMISSVERFIHHVNTCMRQRQERQRLAGVVSRIDAYEVVEGSNDEVDKFLKEFLHLDLTAPMPGTSPEETRQLLLEGSLRMKEGKDSKMDVYCFLFTDLLLVTKAVKKAERTKVIRPPLLVDKIVCRELRDPGSFLLIHLNEFHSAVGAYTFQASSQALCRSWVDTLYNAQNQLQQLRAQLLCAQEHPGTQHLQSLEEEEDEQEEEGEESGTSAASSPTILRKSSNSLDSEHCASDGSTETLAMVVVEPGETLSSPEFDRGPFSSQSDEASLSNTTSSITPTSELLPLGPVDGRSCSMDSAYGTLSPTSLQDFAAPHPVVEPVPVPQTLSPQPSPRLRRRTPVQLLPRLPHLLKSKSEASLLQLLSGTTTSVSPPAPSRSLSELCLITMAPGVRTQSSLQEGGPGWNCPGACGPCQGPPLSESENRPSHKAGGPADSARRKCREMPCGTVPRVQPEPSPGISAQHRKLTLAQLYRIRTTLLLNSTLTASEV q66t02-1 Pleckstrin homology domain-containing family G member 5 SYX1 Plekhg5 Kiaa0720 Syx Synectin-binding RhoA exchange factor protein protein mouse Mus musculus Mouse MGTGPGVSGRRAAARPSSELPSPDSQLLWVGGHAHSSDSQVCHHADCQQLHHRGPLNLCETCDSKFHSTLHYDGHVRFDLPPQGSVLARNVSTRSCPPRTSPAADLEEEEEGCTDGKGDRKSAGLKISKKKARRRHTDDPSKECFTLKFDLNVDIETEIVPAMKKKSLGEVLLPVFERKGIALGKVDIYLDQSNTPLSLTFEAYRFGGHYLRVKAKPGDEGKVEQGVKDSKSLSLPALRPSGAGPPVSERVDPQSRRESSLDILAPGRRRKNMSEFLGEAGIPGHEPPAPSSCSLPVGSSGGTSSGINESWKNRAASRFSGFFSSSPSTSAFSREVDKMEQLESKLHAYSLFGLPRMPRRLRFDHDSWEEEEEDDEEDEESSGLRLEDSWRELTDGHEKLTRRQCHQQEAVWELLHTEVSYIRKLRVITNLFLCCLLNLQESGLLCEVEAERLFSNIPEIAKLHRGLWGSVMVPVLEKARRTRALLQPSDFLKGFKMFGSLFKPYIRYCMEEEGCMEYMRGLLRDNDLFRAYVTWAEKHQQCQRLKLSDMLAKPHQRLTKYPLLLKSVLRKTDDPRTKEAIVTMISSVERFIHHVNTCMRQRQERQRLAGVVSRIDAYEVVEGSNDEVDKLLKEFLHLDLTAPMPGTSPEETRQLLLEGSLRMKEGKDSKMDVYCFLFTDLLLVTKAVKKAERTKVIRPPLLVDKIVCRELRDPGSFLLIYLNEFHSAVGAYTFQASSQALCRSWVDTIYNAQNQLQQLRAQLSAQEHPGSQHLQSLEEEEDEQEEEGEESGTSAASSPTILRKSSNSLDSEHCTSDGSTETLAMVVVEPGATLSSPEFEGGPVSSQSDESSLSNTASSVTPTSELLPLGPVDGRSCSMDSAYGTLSPTSLQDFVAPHPVVEPAPVPQTPSPQPSPRLRRRTPVQLLPRPPRLLKSKSEASLLQLLSGTPAARGVPPAPSRSLSELCLISVAPGVRTQRPLQEGGPGWNGPGMCDPCHGPQLSESENRPSHMTGGPADSARRRCREMPSGTMSRVQSEPPSGVSAQHRKLTLAQLYRIRTTLLLNSTLTASEV myh6_rat Myosin-6 Myh6 Myosin heavy chain 6 Myosin heavy chain, cardiac muscle alpha isoform protein protein rat Rattus norvegicus (Rat) Rat MTDAQMADFGAARYLRKSEKERLEAQTRPFDIRTECFVPDDKEEYVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKDNPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLGFTAEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSGKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKMVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLTRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSHMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALIWQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSARHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNAQAHLKDTQLQLDDAVRANDDLKENIAIVERRNTLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLVRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE plekhg5-gipc1 1476884 n/a Synectin 1476887 unspecified role unspecified role prey prey n/a 1476888 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1c gipc1-plekhg5 1476884 n/a Synectin 1476887 unspecified role unspecified role bait bait n/a 1476888 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1c plekhg5-gipc1-1 1476885 n/a Syx1 1476889 unspecified role unspecified role bait bait n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false lig_pdz_class_1 motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a Synectin 1476887 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1d plekhg5-myh6 1476885 n/a 1476890 unspecified role unspecified role prey prey n/a Syx1 1476889 unspecified role unspecified role bait bait lig_pdz_class_1 motif required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1d gipc1-plekhg5-1 1476886 n/a Syx 1476888 unspecified role unspecified role neutral component neutral component n/a Synectin 1476887 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2a Colocalization is observed upon stimulation of cells with 1x10e-6 molar lysophosphatidic acid (LPA) for 10 minutes.