IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mishima-2002-3 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-5 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-8 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-1 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-6 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-7 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation mishima-2002-9 Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis weight by staining molecular weight estimation by staining weight by staining Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation q9xus9_caeel cyk-4 CELE_K08E3.6 K08E3.6 protein protein caeel Caenorhabditis elegans MKSSTSKEKVCGENSRHIFNMILNSQRPQFDIKDIGMFHLIDEIERLRKLWKDSEESKKRLNADMREAEEALAKARKKLAMFDIDVKDTQKHLRALMEENKALKLDLNVYETREKQLKDAMKNGIFNSLTKEDRDQFKFLHEPLVRTYSKRVQQRHPHLMEDTQDDEDDSEVDYDETGDSFEEVIHLRNGREVRRSSAAGNAVGGKRRSASAHAITAAANSKRSRSRVMTATIDEEPNEGGTPPKRCRDDGSTPHQEMTTTTTTTTTTTIHNSRAQNQDPPRVSLHRQLTRRSLSCGSIPSCDQTPGQTTNNIGLGMSSAILTKSTLDIRTLKRGTPAWTNGTTRDIAMRPHTFIEAGIKAMRKCDKCATALKLATSMKCRDCHQVVHRSCCNKLHLPCIPRPKTMMTPKSALRGAKPGAGEFRLQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQELIVAANLYSTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPVKQSQSQAIAGRDATDCHRAMTALFEFDDVYWQRFLGTSAVSMASNQIETARHQDNFALCDRSILGPVTTSPATPLLARSANATRARGAHLLGSMFHD q8mq03_caeel Kinesin-like protein zen-4 CELE_M03D4.1 M03D4.1 protein protein caeel Caenorhabditis elegans MSSRKRGITPSRDQVRRKKLSIEETDSIEVVCRLCPYTGSTPSLIAIDEGSIQTVLPPAQFRRENAPQVEKVFRFGRVFSENDGQATVFERTSVDLILNLLKGQNSLLFTYGVTGSGKTYTMTGKPTETGTGLLPRTLDVIFNSINNRVEKCIFYPSALNTFEIRATLDAHLKRHQMAADRLSTSREITDRYCEAIKLSGYNDDMVCSVFVTYVEIYNNYCYDLLEDARNGSRVLTKREIRHDRQQQMYVDGAKDVEVSSSEEALEVFCLGEERRRVSSTLLNKDSSRSHSVFTIKLVMAPRAYETKSVYPTMDSSQIIVSQLCLVDLAGSERAKRTQNVGERLAEANSINQSLMTLRQCIEVLRRNQKSSSQNLEQVPYRQSKLTHLFKNYLEGNGKIRMVICVNPKPDDYDENMSALAFAEESQTIEVKKQVERMPSERIPHSFFTQWNSELDGSVRMEDDGSREIPCPPTFCLTDCNDKDTVDSMYKYARKLSSLQNSSEEGPSSTLLTMIRQYMMEADYQRVEIARLKDSLNDKDEEIKKLRGFCSRYKRENASMKERIASCEQGEQENALVMEKLMEQKMEDRKIIQSQKKAMRNVRGIIDNPSPSVASLRSRFDQENVAHPTAPIQTPPPPYQTPGRAPVFKKRLEATTSTTVMSGSSSGGSGQQGYVNPKYQRRSKSASRLLDHQPLHRVPTGTVLQSRTPANAIRTTKPEMHQLNKSGEYRLTHQEVDDEGNISTNIVKAPSKPPELQLEDSQSSSYSPCTDVKSITIYSNHDVEEEDE rgap1_human Rac GTPase-activating protein 1 Male germ cell RacGap RACGAP1 KIAA1478 MGCRACGAP Protein CYK4 homolog protein protein human Homo sapiens Human MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLSEEQKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIETVPYWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK kif23_human Kinesin-like protein KIF23 KIF23 KNSL5 MKLP1 Mitotic kinesin-like protein 1 Kinesin-like protein 5 protein protein human Homo sapiens Human MKSARAKTPRKPTVKKGSQTNLKDPVGVYCRVRPLGFPDQECCIEVINNTTVQLHTPEGYRLNRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSPGEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMPNPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPIKPKPPQSKLLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQMYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVDKAICGLTPGRRYRNQPRGPVGNEPLVTDVVLQSFPPLPSCEILDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSVSPSPVPLSSNYIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKIPTYNTPLKVTSIARRRQQEPGQSKTCIVSDRRRGMYWTEGREVVPTFRNEIEIEEDHCGRLLFQPDQNAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP zen_4-cyk_4-1 1549014 n/a 1549021 unspecified role unspecified role prey prey Q9XUS9:p.Ser15Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1549022 unspecified role unspecified role bait bait in vitro In vitro physical association physical association false false false Fig. 2A/E cyk_4-zen_4-2 1549014 n/a 1549021 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1549022 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_chitin binding tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false in vitro In vitro physical association physical association false false false Fig. 1C cyk_4-zen_4-4 1549014 n/a 1549021 unspecified role unspecified role bait bait in vitro In vitro n/a 1549022 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro physical association physical association false false false Fig. 2C zen_4-cyk_4-3 1549014 n/a 1549022 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1549021 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9XUS9:p.Ser15Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1549047 1549044 physical association physical association false false false Fig. 3B The suppressor mutation (E502K) in ZEN-4 partially recovered the interaction of the CYK-4 S15L mutant with ZEN-4 (z4). cyk_4-cyk_4 1549014 n/a 1549021 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 1549021 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 1549055 1549052 physical association physical association false false false 4A racgap1-kif23 1549015 n/a 1549023 unspecified role unspecified role bait bait n/a 1549024 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5A kif23-racgap1-3 1549015 n/a 1549024 unspecified role unspecified role bait bait n/a 1549023 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5A kif23-racgap1-7 1549016 n/a 1549023 unspecified role unspecified role prey prey n/a 1549024 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5E The complex was immunoprecipitated from a microtubule binding fraction of mitotic HeLa cells racgap1-kif23-1 1549016 n/a 1549023 unspecified role unspecified role bait bait n/a 1549024 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5E The complex was immunoprecipitated from a microtubule binding fraction of mitotic HeLa cells cyk_4-zen_4 1549017 n/a 1549021 unspecified role unspecified role bait bait n/a 1549022 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A kif23-racgap1-4 1549018 n/a 1549024 unspecified role unspecified role neutral component neutral component n/a 1549023 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5B In early anaphase, KIF23 and RACGAP1 localize to the nascent central spindle. During mid-to-late anaphase they localize to short segments in the central spindle kif23-racgap1-5 1549019 n/a 1549023 unspecified role unspecified role neutral component neutral component n/a 1549024 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5D cyk_4-zen_4-7 1549020 n/a 1549022 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 1549021 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction false false false Fig. 6A