IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ng-1996a-1 The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Ng J.Y., Marians K.J. All gel filtration experiments contained single-standed DNA to which the proteins bound during complex-formation. J. Biol. Chem. (0021-9258) 1996 Only protein-protein interactions imex curation dnac_ecoli DNA replication protein DnaC dnaC dnaD b4361 JW4325 protein protein ecoli Escherichia coli (strain K12) MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAALERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASFIFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDLSNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVMDRMRLGNSLWVIFNWDSYRSRVTGKEY prib_ecoli Primosomal replication protein N priB b4201 JW4159 protein protein ecoli Escherichia coli (strain K12) MTNRLVLSGTVCRAPLRKVSPSGIPHCQFVLEHRSVQEEAGFHRQAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDSGD pria_ecoli Primosomal protein N' priA b3935 Replication factor Y JW3906 ATP-dependent helicase PriA protein protein ecoli Escherichia coli (strain K12) MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEFNDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG dnab_ecoli Replicative DNA helicase dnaB groP grpA b4052 JW4012 protein protein ecoli Escherichia coli (strain K12) MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE pric_ecoli Primosomal replication protein N'' priC b0467 JW0456 protein protein ecoli Escherichia coli (strain K12) MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR dnat_ecoli Primosomal protein 1 dnaT b4362 JW4326 Primosomal protein I protein protein ecoli Escherichia coli (strain K12) MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG 8DE048A3B2A6235B dnag_ecoli DNA primase dnaG dnaP parB b3066 JW3038 protein protein ecoli Escherichia coli (strain K12) MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNEERLELWTLNQELAKK 294CF020E7B45D94 ssdna-1 Single-stranded deoxyribonucleic acid ss dna single stranded deoxyribonucleic acid ss DNA ss dna ecoli Escherichia coli (strain K12) preprimosome 1373238 n/a 1373239 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373240 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373241 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373242 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373243 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373244 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1a, 2, 3 DnaC and PriC form very weak interactions with the other preprimosome proteins. primosome 1373238 n/a 1373240 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373244 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373242 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373243 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373241 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373245 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373239 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3, 4, 6, 8 DnaC and PriC form very weak interactions with the other primosome proteins. preprimosome-ssdna 1373238 n/a 1373240 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373244 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373243 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373241 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373246 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1373242 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1a dnab-dnac-dnat-pric 1373238 n/a 1373244 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373239 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373242 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1373243 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1a, b prib-ssdna 1373238 n/a 1373246 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1373240 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1b