MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint david-2013-1 Quality control of a molybdoenzyme by the Lon protease. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined David Redelberger; Olivier Genest; Dallel Arabet; Vincent Mejean; Marianne Ilbert; chantal iobbi-nivol iobbi@imm.cnrs.fr 2013 Only protein-protein interactions imex curation David Redelberger, Olivier Genest, Dallel Arabet, Vincent Mejean, Marianne Ilbert, chantal iobbi-nivol, Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C., Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C. FEBS letters david-2013-2 Quality control of a molybdoenzyme by the Lon protease. in vitro In vitro enzymatic study enzymatic study western blot western blot Immuno blot Immuno blot David Redelberger; Olivier Genest; Dallel Arabet; Vincent Mejean; Marianne Ilbert; chantal iobbi-nivol iobbi@imm.cnrs.fr 2013 Only protein-protein interactions imex curation David Redelberger, Olivier Genest, Dallel Arabet, Vincent Mejean, Marianne Ilbert, chantal iobbi-nivol, Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C., Redelberger D., Genest O., Arabet D., Méjean V., Ilbert M., Iobbi-Nivol C. FEBS letters tora_ecoli Trimethylamine-N-oxide reductase 1 torA b0997 JW0982 protein protein ecoli Escherichia coli (strain K12) MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAATDAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRYPMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGWQSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTHEYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEKDGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLAAMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGLRKLETVIAIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS lon_ecoli Lon protease lon capR deg lopA muc b0439 ATP-dependent protease La JW0429 protein protein ecoli Escherichia coli (strain K12) MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSIDDPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIRGHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLPKQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLKHIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK tora-lon 1454359 n/a apoTorA 1454361 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Lon 1454362 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1454359 f2 The first set of results suggests that Lon recognizes only the core of apoTorA. To confirm this point, a surface plasmon resonance (SPR) experiment was carried out to follow, in real time, the interaction between Lon and the apo or holo form of TorA. The data show an interaction between Lon and apoTorA (Fig. 2),. Moreover, this data set shows the formation of a very stable complex between Lon and apoTorA. The determination of the equilibrium constant level (Req) at different concentrations allows us to estimate after three independent experiments a Kd of 1.0 ± 0.2 μM. In contrast, no interaction was detected with holoTorA (Fig.2 inset) confirming that the protease binds the core of TorA only in the absence of the cofactor. lon-tora 1454360 n/a apoTorA 1454361 predetermined predetermined participant predetermined enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Lon 1454362 predetermined predetermined participant predetermined enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false cleavage cleavage reaction cleavage false false false f4a f4c