IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ong-2013-3 Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila. drome-larva Drosophila melanogaster larva larva Immature form of an organism fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Ong C.T., Van Bortle K., Ramos E., Corces V.G. vcorces@emory.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation ong-2013-1 Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ong C.T., Van Bortle K., Ramos E., Corces V.G. vcorces@emory.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation ong-2013-2 Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ong C.T., Van Bortle K., Ramos E., Corces V.G. vcorces@emory.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation parp_drome Poly [ADP-ribose] polymerase Parp Poly[ADP-ribose] synthase NAD(+) ADP-ribosyltransferase Protein ADP-ribosyltransferase Parp CG40411 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDIELPYLAEYARTGRATCKGCKSTISKDTLRIAVMVQSAFHDAKVPNWFHKTCFFKNQRPSSVGDIQNIGNLRFADQKELTDLVENIQEVISAQLGKKRSKAFNLALKDFGIEYAKSSRSTCRGCEQKINKDLVRLRKTVYDTEVGMKYGGQPLWHHLECFAQLRSELGWFASGEDMPGFQSLADDDQAKVKNAIPPIKSEELPDTKRAKMELSDTNEEGEKKQRLKDQNDAYFRFRDDIKNKMKKKDIDILLKFNNQQPVTGDTEKLFDQTADLLTFGAIESCSECNSCQFIVNKSGYICNGNHSEWTKCNKLLKEPTRSACIVPKELKALYNFLNTVKEIPSTRIFNNFPPNKSTFSRSLLKTNKNNDVLVRPTIPRISPPLYNLKFSIIGLKNQHKELRKRIENLGGKFEVKISENTIAIISTELEIQKKSTRMKFAEELGIHIVPIEFLDFVEADTEGAIKYINSTCICSWGTDPKSRIPKETTKSLNSNSIYTKSMPVSRTFKVKDGLAVDPDSGLEDIAHVYVDSNNKYSVVLGLTDIQRNKNSYYKVQLLKADKKEKYWIFRSWGRIGTNIGNSKLEEFDTSESAKRNFKEIYADKTGNEYEQRDNFVKRTGRMYPIEIQYDDDQKLVKHESHFFTSKLEISVQNLIKLIFDIDSMNKTLMEFHIDMDKMPLGKLSAHQIQSAYRVVKEIYNVLECGSNTAKLIDATNRFYTLIPHNFGVQLPTLIETHQQIEDLRQMLDSLAEIEVAYSIIKSEDVSDACNPLDNHYAQIKTQLVALDKNSEEFSILSQYVKNTHASTHKSYDLKIVDVFKVSRQGEARRFKPFKKLHNRKLLWHGSRLTNFVGILSHGLRIAPPEAPPTGYMFGKGIYFADMVSKSANYCCTSQQNSTGLMLLSEVALGDMMECTSAKYINKLSNNKHSCFGRGRTMPDPTKSYIRSDGVEIPYGETITDEHLKSSLLYNEYIVYDVAQVNIQYLFRMEFKYSY cp190_drome Centrosome-associated zinc finger protein CP190 Cp190 E(mod)4-1 CG6384 dMAP190 Protein enhancer of mod(mdg4)4-1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL mmd4_drome Modifier of mdg4 mod(mdg4) bpd doom E(var)3-93D CG32491 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADDEQFSLCWNNFNTNLSAGFHESLCRGDLVDVSLAAEGQIVKAHRLVLSVCSPFFRKMFTQMPSNTHAIVFLNNVSHSALKDLIQFMYCGEVNVKQDALPAFISTAESLQIKGLTDNDPAPQPPQESSPPPAAPHVQQQQIPAQRVQRQQPRASARYKIETVDDGLGDEKQSTTQIVIQTTAAPQATIVQQQQPQQAAQQIQSQQLQTGTTTTATLVSTNKRSAQRSSLTPASSSAGVKRSKTSTSANVMDPLDSTTETGATTTAQLVPQQITVQTSVVSAAEAKLHQQSPQQVRQEEAEYIDLPMELPTKSEPDYSEDHGDAAGDAEGTYVEDDTYGDMRYDDSYFTENEDAGNQTAANTSGGGVTATTSKAVVKQQSQNYSESSFVDTSGDQGNTEAQAATSASATKIPPRKRGRPKTKVEDQTPKPKLLEKLQAATLNEEASEPAVYASTTKGGVKLIFNGHLFKFSFRKADYSVFQCCYREHGEECKVRVVCDQKRVFPYEGEHVHFMQASDKSCLPSQFMPGESGVISSLSPSKELLMKNTTKLEEADDKEDEDFEEFEIQEIDEIELDEPEKTPAKEEEVDPNDFREKIKRRLQKALQNKKK q8ta44_drome CG8591 CTCF protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPRRTKKDEDPEDLQTFLNNFHKEIEGNSDEKVVNTILEAISAEAIDLDENGAEAGGSKPMEEAEADLDHAEEAEEEEEDDEDKYFIDDEGNCYIKTTPKKQKELQKKLKQAAAKPGKATRSVVSTATNKSINLRPAKSTPKATTSKPPPEPKAISVRPARAAAAKAKQSAMPPPPALVVKVPAPRGRPRKNPVIPKPEPMDLERELEELVDEPDISSMVTELSDYTVDEAAVEAATATLTPNEAEVYEFEDNATTEDENADKKDVDFVLSNKEVKLKTASSTSQNSNASGHKYSCPHCPYTASKKFLITRHSRSHDVEPSFKCSICERSFRSNVGLQNHINTHMGNKPHKCKLCESAFTTSGELVRHTRYKHTKEKPHKCTECTYASVELTKLRRHMTCHTGERPYQCPHCTYASQDMFKLKRHMVIHTGEKKYQCDICKSRFTQSNSLKAHKLIHSVVDKPVFQCNYCPTTCGRKADLRVHIKHMHTSDVPITCRRCGQQLPDRYQYKLHVKSHEGEKCYSCKLCSYASVTQRHLASHMLIHLDEKPFHCDQCPQAFRQRQLLRRHMNLVHNEEYQPPEPREKLHKCPSCPREFTHKGYLMRHMETHDDSANAREKRRRLKLGRNVRLQKDGTVITLIKDQYVDMDRDQEENEEDDNPESYDLAEIEPENSEAEDADDDVETIVSDPIRQRIKPAPIIINKQARLAASEKQPMIINQRLRSQRGTKTFHIKEEPDNSDFTVEWQGDDGEVMVVELVNGDEEVLVKHEPSANSKISAKNCFGFEDDDDYEEYGDGENEVDGASQEFLQLMDMIEQDS cp190-parp 1458664 n/a 1458667 unspecified role unspecified role neutral component neutral component n/a 1458668 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig 2A cp190-mod_mdg4_-4 1458664 n/a 1458668 unspecified role unspecified role neutral component neutral component n/a 1458669 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig 2F cp190-ctcf-2 1458665 n/a 1458668 unspecified role unspecified role bait bait n/a 1458670 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3A, Supp. Fig 2I significantly impaired in Parp -/- cells CHEBI:64042 PARP inhibitor 3-aminobenzamide (3AB) cp190-ctcf-3 1458666 n/a 1458670 unspecified role unspecified role prey prey n/a 1458668 unspecified role unspecified role bait bait Q24478:p.Lys566Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3B significantly impaired in Parp -/- cells CHEBI:64042 PARP inhibitor 3-aminobenzamide (3AB)