IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ qiu-2013a-2 Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. yeast Saccharomyces cerevisiae Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Qiu J., Wenz L.S., Zerbes R.M., Oeljeklaus S., Bohnert M., Stroud D.A., Wirth C., Ellenrieder L., Thornton N., Kutik S., Wiese S., Schulze-Specking A., Zufall N., Chacinska A., Guiard B., Hunte C., Warscheid B., van der Laan M., Pfanner N., Wiedemann N., Becker T. nikolaus.pfanner@biochemie.uni-freiburg.de, nils.wiedemann@biochemie.uni-freiburg.de Cell (0092-8674) 2013 Only protein-protein interactions imex curation qiu-2013a-4 Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. yeast Saccharomyces cerevisiae Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Qiu J., Wenz L.S., Zerbes R.M., Oeljeklaus S., Bohnert M., Stroud D.A., Wirth C., Ellenrieder L., Thornton N., Kutik S., Wiese S., Schulze-Specking A., Zufall N., Chacinska A., Guiard B., Hunte C., Warscheid B., van der Laan M., Pfanner N., Wiedemann N., Becker T. Complexes were further purified by BN-PAGE nikolaus.pfanner@biochemie.uni-freiburg.de, nils.wiedemann@biochemie.uni-freiburg.de Cell (0092-8674) 2013 Only protein-protein interactions imex curation qiu-2013a-1 Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. yeast Saccharomyces cerevisiae Baker's yeast pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Qiu J., Wenz L.S., Zerbes R.M., Oeljeklaus S., Bohnert M., Stroud D.A., Wirth C., Ellenrieder L., Thornton N., Kutik S., Wiese S., Schulze-Specking A., Zufall N., Chacinska A., Guiard B., Hunte C., Warscheid B., van der Laan M., Pfanner N., Wiedemann N., Becker T. Tom50-interacting proteins were identified by SILAC but as a high confidence subset could not be identified from the information in the publication, this data was not included. nikolaus.pfanner@biochemie.uni-freiburg.de, nils.wiedemann@biochemie.uni-freiburg.de Cell (0092-8674) 2013 Only protein-protein interactions imex curation qiu-2013a-3 Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Qiu J., Wenz L.S., Zerbes R.M., Oeljeklaus S., Bohnert M., Stroud D.A., Wirth C., Ellenrieder L., Thornton N., Kutik S., Wiese S., Schulze-Specking A., Zufall N., Chacinska A., Guiard B., Hunte C., Warscheid B., van der Laan M., Pfanner N., Wiedemann N., Becker T. SH-specific crosslinking reagent bis(maleimido)ethane (BMOE; 8 A spacer), proteins then pulled down on nickel resin nikolaus.pfanner@biochemie.uni-freiburg.de, nils.wiedemann@biochemie.uni-freiburg.de Cell (0092-8674) 2013 Only protein-protein interactions imex curation qiu-2013a-5 Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Qiu J., Wenz L.S., Zerbes R.M., Oeljeklaus S., Bohnert M., Stroud D.A., Wirth C., Ellenrieder L., Thornton N., Kutik S., Wiese S., Schulze-Specking A., Zufall N., Chacinska A., Guiard B., Hunte C., Warscheid B., van der Laan M., Pfanner N., Wiedemann N., Becker T. SH-specific crosslinking reagent disuccinimidyl glutarate, proteins then pulled down on nickel resin nikolaus.pfanner@biochemie.uni-freiburg.de, nils.wiedemann@biochemie.uni-freiburg.de Cell (0092-8674) 2013 Only protein-protein interactions imex curation tom20_yeast Mitochondrial import receptor subunit TOM20 MOM19 YGR082W TOM20 MAS20 Mitochondrial 20 kDa outer membrane protein Translocase of outer membrane 20 kDa subunit Protein MAS20 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQSNPILRGLAITTAIAALSATGYAIYFDYQRRNSPQFRKVLRQRAKEQAKMEEQAKTHAKEVKLQKVTEFLSMELAKDPIPSDPSEREATFTTNVENGERLSMQQGKELEAASKFYKALTVYPQPADLLGIYQRSIPEAIYEYIILMIAILPPANVASFVKGVVGSKAESDAVAEANDIDD tom7_yeast Mitochondrial import receptor subunit TOM7 TOM7 MOM7 YNL070W N2378 Translocase of outer membrane 7 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFLPSFILSDESKERISKILTLTHNVAHYGWIPFVLYLGWAHTSNRPNFLNLLSPLPSV tom22_yeast Mitochondrial import receptor subunit TOM22 MOM22 TOM22 MAS17 MAS22 YNL131W N1217 N1862 Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN 6ACEC5D37D143CD3 sam35_yeast Sorting assembly machinery 35 kDa subunit YHR083W SAM35 OMP85 TOB38 TOM38 TOB complex 38 kDa subunit Mitochondrial 38 kDa outer membrane protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSSFSVPMPVKRIFDTFPLQTYAAQTDKDEAVALEIQRRSYTFTERGGGSSELTVEGTYKLGVYNVFLEANTGAALATDPWCLFVQLALCQKNGLVLPTHSQEQTPSHTCNHEMLVLSRLSNPDEALPILVEGYKKRIIRSTVAISEIMRSRILDDAEQLMYYTLLDTVLYDCWITQIIFCASDAQFMELYSCQKLSGSIVTPLDVENSLLQKLSAKSLKISLTKRNKFQFRHREIVKSMQGVYHNHHNSVNQEQVLNVLFENSKQVLLGLKDMLKSDGQPTYLHLKIASYILCITNVKEPIKLKTFVENECKELVQFAQDTLKNFVQ sam37_yeast Sorting assembly machinery 37 kDa subunit SAM37 MAS37 PET3027 TOM37 Mitochondrial 37 kDa outer membrane protein MAS37 protein YM9796.13C YMR060C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVKGSVHLWGKDGKASLISVDSIALVWFIKLCTSEEAKSMVAGLQIVFSNNTDLSSDGKLPVLILDNGTKVSGYVNIVQFLHKNICTSKYEKGTDYEEDLAIVRKKDRLLEYSLLNYVDVEISRLTDYQLFLNTKNYNEYTKKLFSKLLYFPMWYNTPLQLRSQARENCEEIIGSLTLEDDEEFVESKAMESASQLAQSKTFKIAHKNKIKGKQELQQVKYNLQFDNRLQSCVSNWLAARKKLDDSVILSSDLLFLANLYVQLGLPDGNRIRSKLEQTFGSELLNSMSNKIDDFVHRPSNNLEQRDPQFREQGNVVMSLYNLACKYI sam50_yeast Sorting assembly machinery 50 kDa subunit SAM50 YNL026W N2802 TOB55 TOB complex 55 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSSSGVDNEISLDSPMPIFNESSTLKPIRVAGVVTTGTDHIDPSVLQAYLDDTIMKSITLGQLVKNADVLNKRLCQHHIALNAKQSFHFQGNTYISDEKETHDVVPLMEVVSQLDILPPKTFTAKTGTNFGNDNDAEAYLQFEKLIDKKYLKLPTRVNLEILRGTKIHSSFLFNSYSSLSPQSILNLKVFSQFYNWNTNKGLDIGQRGARLSLRYEPLFLHKLLHNPHSNESPTLFHEWFLETCWRSTKICSQGTSAPYMYSGTMLSQAGDQLRTILGHTFVLDKRDHIMCPTKGSMLKWSNELSPGKHLKTQLELNSVKSWMNDDFITFSTTIKTGYLKNLSSQQSLPVHICDKFQSGGPSDIRGFQTFGLGPRDLYDAVGGDAFVSYGLSVFSRLPWKKVEKSNFRLHWFFNGGKLVNHDNTSLGNCIGQLSKEHSTSTGIGLVLRHPMARFELNFTLPITAHENDLIRKGFQFGLGLAFL tom40_yeast Mitochondrial import receptor subunit TOM40 TOM40 ISP42 YMR203W YM8325.04 MOM38 Mitochondrial import site protein ISP42 Translocase of outer membrane 40 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL tom70_yeast Mitochondrial import receptor subunit TOM70 TOM70 MAS70 YNL121C N1905 OMP1 70 kDa mitochondrial outer membrane protein Translocase of outer membrane 70 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSFITRNKTAILATVAATGTAIGAYYYYNQLQQQQQRGKKNTINKDEKKDTKDSQKETEGAKKSTAPSNPPIYPVSSNGEPDFSNKANFTAEEKDKYALALKDKGNQFFRNKKYDDAIKYYNWALELKEDPVFYSNLSACYVSVGDLKKVVEMSTKALELKPDYSKVLLRRASANEGLGKFADAMFDLSVLSLNGDFNDASIEPMLERNLNKQAMSKLKEKFGDIDTATATPTELSTQPAKERKDKQENLPSVTSMASFFGIFKPELTFANYDESNEADKELMNGLSNLYKRSPESYDKADESFTKAARLFEEQLDKNNEDEKLKEKLAISLEHTGIFKFLKNDPLGAHEDIKKAIELFPRVNSYIYMALIMADRNDSTEYYNYFDKALKLDSNNSSVYYHRGQMNFILQNYDQAGKDFDKAKELDPENIFPYIQLACLAYRENKFDDCETLFSEAKRKFPEAPEVPNFFAEILTDKNDFDKALKQYDLAIELENKLDGIYVGIAPLVGKATLLTRNPTVENFIEATNLLEKASKLDPRSEQAKIGLAQMKLQQEDIDEAITLFEESADLARTMEEKLQAITFAEAAKVQQRIRSDPVLAKKIQETLAKLREQGLM 664B8FC32CCE39A2 tom5_yeast Mitochondrial import receptor subunit TOM5 YPR133W-A TOM5 MOM8A Translocase of outer membrane 5 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGLPQQEVSEEEKRAHQEQTEKTLKQAAYVAAFLWVSPMIWHLVKKQWK E1419ED5FEC45E14 vdac1_yeast Mitochondrial outer membrane protein porin 1 POR1 OMP2 VDAC1 YNL055C N2441 YNL2441C Voltage-dependent anion-selective channel protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSPPVYSDISRNINDLLNKDFYHATPAAFDVQTTTANGIKFSLKAKQPVKDGPLSTNVEAKLNDKQTGLGLTQGWSNTNNLQTKLEFANLTPGLKNELITSLTPGVAKSAVLNTTFTQPFFTARGAFDLCLKSPTFVGDLTMAHEGIVGGAEFGYDISAGSISRYAMALSYFAKDYSLGATLNNEQITTVDFFQNVNAFLQVGAKATMNCKLPNSNVNIEFATRYLPDASSQVKAKVSDSGIVTLAYKQLLRPGVTLGVGSSFDALKLSEPVHKLGWSLSFDA C99D03711AF9B54D mdm10_yeast Mitochondrial distribution and morphology protein 10 MDM10 YAL010C FUN37 Mitochondrial inheritance component MDM10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLPYMDQVLRAFYQSTHWSTQNSYEDITATSRTLLDFRIPSAIHLQISNKSTPNTFNSLDFSTRSRINGSLSYLYSDAQQLEKFMRNSTDIPLQDATETYRQLQPNLNFSVSSANTLSSDNTTVDNDKKLLHDSKFVKKSLYYGRMYYPSSDLEAMIIKRLSPQTQFMLKGVSSFKESLNVLTCYFQRDSHRNLQEWIFSTSDLLCGYRVLHNFLTTPSKFNTSLYNNSSLSLGAEFWLGLVSLSPGCSTTLRYYTHSTNTGRPLTLTLSWNPLFGHISSTYSAKTGTNSTFCAKYDFNLYSIESNLSFGCEFWQKKHHLLETNKNNNDKLEPISDELVDINPNSRATKLLHENVPDLNSAVNDIPSTLDIPVHKQKLLNDLTYAFSSSLRKIDEERSTIEKFDNKINSSIFTSVWKLSTSLRDKTLKLLWEGKWRGFLISAGTELVFTRGFQESLSDDEKNDNAISISATDTENGNIPVFPAKFGIQFQYST 5AE98366B5EF25E5 sam50-sam35-1 1445838 n/a 1445843 unspecified role unspecified role prey prey n/a 1445844 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role prey prey n/a 1445846 unspecified role unspecified role prey prey n/a 1445847 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445849 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4B Ongoing protein synthesis is not essential for supercomplex formation. tom22-sam35-3 1445838 n/a 1445848 unspecified role unspecified role prey prey n/a 1445850 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445847 unspecified role unspecified role prey prey n/a 1445849 unspecified role unspecified role prey prey n/a 1445843 unspecified role unspecified role prey prey n/a 1445844 unspecified role unspecified role prey prey n/a 1445846 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4C sam37-sam35-1 1445838 n/a 1445843 unspecified role unspecified role prey prey n/a 1445849 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role prey prey n/a 1445847 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445844 unspecified role unspecified role prey prey n/a 1445846 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role prey prey n/a 1445851 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 4A sam37-sam35-2 1445838 n/a 1445843 unspecified role unspecified role prey prey n/a 1445846 unspecified role unspecified role prey prey n/a 1445847 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445844 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role prey prey n/a 1445849 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 5A, 5B Interaction observed in Tom5-/- cells. Interaction observed in Tom20-/- cells. Tom70, which is only loosely associated with the TOM complex was not found in stable association with the TOM-SAM supercomplex. sam37-sam35-3 1445838 n/a 1445846 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role prey prey n/a 1445844 unspecified role unspecified role prey prey n/a 1445849 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role prey prey n/a 1445847 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445851 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 4A Interaction observed in Tom20-/- cells. tom22-sam35-2 1445838 n/a 1445847 unspecified role unspecified role prey prey n/a 1445846 unspecified role unspecified role prey prey n/a 1445845 unspecified role unspecified role bait bait cytoplasmic region sufficient to bind sufficient binding region sufficient to bind range range range range false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445848 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6F sam50-sam35-2 1445838 n/a 1445848 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445843 unspecified role unspecified role prey prey n/a 1445849 unspecified role unspecified role prey prey physical association physical association false false false Supp. fig. 5A Ongoing protein synthesis is not essential for supercomplex formation. por1-sam35 1445838 n/a 1445846 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role prey prey n/a 1445852 unspecified role unspecified role bait bait P04840:p.Phe281Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false sam50-mdm10-1 1445839 n/a 1445845 unspecified role unspecified role prey prey n/a 1445853 unspecified role unspecified role prey prey n/a 1445848 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445849 unspecified role unspecified role prey prey association association false false false Fig. 4D, Supp Fig. 5A (right) Ongoing protein synthesis is not essential for supercomplex formation sam37-mdm10 1445839 n/a 1445853 unspecified role unspecified role prey prey n/a 1445847 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1445845 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4D sam50-tom40 1445840 n/a 1445849 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P23644:p.[Asp87Cys;Cys165Trp;Cys326Ala;Cys341Ser;Cys355Phe;Gln358Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1445848 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Sam50ProtA pulled down both reduced and oxidized Tom40 Fig. 2C sam50-tom22 1445841 n/a 1445848 unspecified role unspecified role neutral component neutral component n/a 1445845 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6G sam50-tom22-1 1445842 n/a 1445848 unspecified role unspecified role neutral component neutral component n/a 1445845 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6D