IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-2013c-2 Visualizing GroEL/ES in the act of encapsulating a folding protein. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chen D.H., Madan D., Weaver J., Lin Z., Schröder G.F., Chiu W., Rye H.S. wah@bcm.edu, haysrye@tamu.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation chen-2013c-1 Visualizing GroEL/ES in the act of encapsulating a folding protein. in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Chen D.H., Madan D., Weaver J., Lin Z., Schröder G.F., Chiu W., Rye H.S. wah@bcm.edu, haysrye@tamu.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 ch10_ecoli Co-chaperonin GroES groES 10 kDa chaperonin Chaperonin-10 groS mopB JW4102 b4142 protein protein ecoli Escherichia coli (strain K12) MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA 76829E09B11217EF gfp_aeqvi Green fluorescent protein GFP protein protein aeqvi Aequorea victoria MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK rlp_rhort 5-methylthioribulose-1-phosphate isomerase rlp Rru_A1998 protein protein rhort Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255) MTDRLRATYRVKATAASIEARAKGIAVEQSVEMPLSAIDDPAVLDGIVGVVEEITERGEDCFEVRLALSTATIGGDAGQLFNMLFGNTSLQDDTVLLDIDLPDDLLASFGGPNIGAAGLRARVGASADRALTCSALKPQGLPPDRLADLARRMALGGLDFIKDDHGMADQAYAPFASRVGAVAAAVDEVNRQTGGQTRYLPSLSGHLDQLRSQVRTGLDHGIDTFLIAPMIVGPSTFHAVVREFPGAAFFAHPTLAGPSRIAPPAHFGKLFRLLGADAVIFPNSGGRFGYSRDTCQAVAEAALGPWGGLHASLPVPAGGMSLARVPEMIATYGPDVIVLIGGNLLEARDRLTEETAAFVASVAGAASRGCGLAP thtr_bovin Thiosulfate sulfurtransferase TST Rhodanese protein protein bovin Bos taurus (Bovine) Bovine MVHQVLYRALVSTKWLAESVRAGKVGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDKASPYEVMLPSEAGFADYVGSLGISNDTHVVVYDGDDLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAIFKATLNRSLLKTYEQVLENLESKRFQLVDSRAQGRYLGTQPEPDAVGLDSGHIRGSVNMPFMNFLTEDGFEKSPEELRAMFEAKKVDLTKPLIATCRKGVTACHIALAAYLCGKPDVAIYDGSWFEWFHRAPPETWVSQGKGGKA mint Formation of iron-sulfur complexes and cyanide detoxification. Binds molecular oxygen and sulfur atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gfp-grol 1440893 n/a 1440895 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1440897 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 6C grol-gros-12 1440893 n/a 1440895 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1440898 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 6E grol-gros-13 1440893 n/a 1440899 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1440896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) n/a 1440895 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 6D atp-grol-1 1440894 n/a 1440900 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 7.0 n/a 1440896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 7.0 n/a 1440895 unspecified role unspecified role neutral component neutral component P0A6F5:p.Ser43Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-43 pyrene label pyrene label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0A6F5:p.Asp398Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 14.0 physical association physical association false false false 8.9A Fig 2 atp-grol-3 1440894 n/a 1440898 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 n/a 1440896 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 7.0 n/a 1440900 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 7.0 n/a 1440895 unspecified role unspecified role neutral component neutral component cys-43 pyrene label pyrene label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0A6F5:p.Asp398Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false P0A6F5:p.Ser43Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 14.0 physical association physical association false false false 9.2A (EMD-2326), 15.9A (EMD-2327) Fig 3 The entry in EMD lists the RuBisCo protein as an interactor, the corresponding model in PDB does not. The RuBisCo construct has been mapped to the Rhodospirillum rubrum reference strain