IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ thyrock-2013-4 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. A Mint1 isoform1 PTB domain containing construct (aas 438-625) also did not bind Rab6 proteins, - same as for the full length Mint1 isoform. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-6 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-9 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease This experiment relates to negative interactions. FACS was used for fluorescence detection. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease q02410-1 Amyloid-beta A4 precursor protein-binding family A member 1 APBA1 MINT1 X11 Adapter protein X11alpha Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 protein protein human Homo sapiens Human MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI p20340-1 Ras-related protein Rab-6A Rab-6a RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC rab6b_human Ras-related protein Rab-6B RAB6B protein protein human Homo sapiens Human MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC rab6a_human Ras-related protein Rab-6A RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC rab6b-apba1-3 1454747 n/a 1454750 unspecified role unspecified role prey prey n/a 1454751 unspecified role unspecified role bait bait P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P20340-1:p.Thr27Asn mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false true TABLE 2 and 4 The interaction does not occur under these experimental conditions. rab6b-apba1-3 1454747 n/a 1454752 unspecified role unspecified role bait bait Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9NRW1:p.Thr27Asn mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1454750 unspecified role unspecified role prey prey physical association physical association false false true TABLE 2 and 4 The interaction does not occur under these experimental conditions. q02410_2-rab6b-1 1454748 n/a 1454752 unspecified role unspecified role prey prey Q9NRW1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1454750 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ptb/pi_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false true Fig 2A This interaction does not occur under these experimental conditions. q02410_2-rab6a-1 1454748 n/a 1454753 unspecified role unspecified role prey prey region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P20340:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P20340:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1454750 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ptb/pi_domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false true Fig 2A This interaction does not occur under these experimental conditions. q02410_2-rab6b-3 1454749 n/a 1454750 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1454752 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false true Fig 3 The interaction does not occur under these experimental conditions. q02410_2-rab6a-5 1454749 n/a 1454750 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1454751 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false true Fig 3 The interaction does not occur under these experimental conditions.