IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ thyrock-2013-7 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-3 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-5 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-8 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease FACS was used for fluorescence detection. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-1 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-2 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurons obtained from Hippocampal cultures. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-10 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurons obtained from Hippocampal cultures. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-11 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurons obtained from Hippocampal cultures. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-12 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease thyrock-2013-13 A new Mint1 isoform, but not the conventional Mint1, interacts with the small GTPase Rab6. chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Thyrock A., Ossendorf E., Stehling M., Kail M., Kurtz T., Pohlentz G., Waschbüsch D., Eggert S., Formstecher E., Müthing J., Dreisewerd K., Kins S., Goud B., Barnekow A. Parkinsons - Interactions investigated in the context of Parkinsons disease Live cell imaging. The authors confirm that Rab6B constructs/proteins are of human origin, and also that the species of transfected APP695 in Figure 7 and Figure 9C is human. barneko@uni-muenster.de PLoS ONE 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease q02410-2 Amyloid-beta A4 precursor protein-binding family A member 1 Mint1_826 APBA1 MINT1 X11 Adapter protein X11alpha Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 protein protein human Homo sapiens Human MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI This isoform interacts with RAB6 GTPases. p20340-1 Ras-related protein Rab-6A Rab-6a RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC rab6b_human Ras-related protein Rab-6B RAB6B protein protein human Homo sapiens Human MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC apba1_mouse Amyloid-beta A4 precursor protein-binding family A member 1 Apba1 Mint1 X11 Adapter protein X11alpha Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 mLin-10 protein protein mouse Mus musculus Mouse MNHLEGSAEVEVADEAPGGEVNESVEADLEHPEVVEGQQPSPSPPPPAGHEPEDHRGHPAPPPPPPPQEEEEEERGECLARSASTESGFHNHTDTAEGDVLAAARDGYEAERAQDADDESAYAVQYRPEAEEYTEQAEAEHVEAAQRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRAEDEPYAEPYADYGGLQEHVYEEIGDAPELEARDGLRLYERERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERAPTPGGGHPDSPGLPAPAGQQQRVVGTPGGSEVGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPVDGDSPSPGSSSPLGAESSSIPLHPGDPTEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKTAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQEPMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI p05067-4 Amyloid-beta precursor protein APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI PreA4 695 Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human MLPGLALLLLAAWTARALEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVRVPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN A major isoform. p20340-2 Ras-related protein Rab-6A Rab-6a' Rab-6C Rab6C RAB6A RAB6 protein protein human Homo sapiens Human MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC apba1_human Amyloid-beta A4 precursor protein-binding family A member 1 APBA1 MINT1 X11 Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 Adapter protein X11alpha protein protein human Homo sapiens Human MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI rab6b_mouse Ras-related protein Rab-6B Rab6b D9Bwg0185e protein protein mouse Mus musculus Mouse MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC rab6a_mouse Ras-related protein Rab-6A Rab6a Rab6 MNCb-1660 protein protein mouse Mus musculus Mouse MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVNEGGCSC rab6a-q02410_2 1454630 n/a 1454640 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) n/a 1454641 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P20340-1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Fig 2B rab6b-q02410_2 1454630 n/a 1454642 unspecified role unspecified role bait bait Q9NRW1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1454640 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 2B rab6a-q02410_2-1 1454630 n/a 1454641 unspecified role unspecified role bait bait P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P20340-1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1454643 unspecified role unspecified role prey prey mouse-brain Mus musculus brain brain Brain [cerebrum] direct interaction direct interaction false false false Fig 5 The antibody used can detect both isoforms of MINT1 but the authors state that as only Mint1 826 but not Mint1 can bind to Rab6, this pulldown experiment indicates that the Mint1 826 protein is present in mouse brain tissue and that this endogenous Mint1 826 can interact with Rab6. q02410_2-rab6a-6 1454630 n/a 1454640 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1454644 unspecified role unspecified role prey prey mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. physical association physical association false false false Fig 7 rab6b-apba1-2 1454631 n/a 1454640 unspecified role unspecified role prey prey ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1454642 unspecified role unspecified role bait bait Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9NRW1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false TABLE 1 and 3,4 rab6b-apba1-2 1454631 n/a 1454640 unspecified role unspecified role prey prey ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1454641 unspecified role unspecified role bait bait P20340-1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false TABLE 1 and 3,4 rab6a-apba1-1 1454631 n/a 1454645 unspecified role unspecified role bait bait P20340-2:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1454640 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false TABLE 1 q02410_2-rab6b 1454632 n/a 1454640 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1454642 unspecified role unspecified role prey prey Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9NRW1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Fig 2A q02410_2-rab6a 1454632 n/a 1454641 unspecified role unspecified role prey prey c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false P20340-1:p.Thr27Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1454640 unspecified role unspecified role bait bait ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2A q02410_2-rab6a-4 1454633 n/a 1454641 unspecified role unspecified role fluorescence donor fluorescence donor P20340-1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1454640 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig 3 q02410_2-rab6b-2 1454633 n/a 1454642 unspecified role unspecified role fluorescence donor fluorescence donor Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false c_terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1454640 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept ptb/pi_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus eyfp enhanced yellow fluorescent protein tag eyfp n-term range n-terminal range n-term range n-term range n-terminal range n-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false Fig 3 rab6b-apba1 1454634 n/a 1454646 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1454642 unspecified role unspecified role bait bait Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure or table A (prey) clone was isolated that contained a fragment, which was identified as Mint1 (pP6 Mint1, aa 438–769) by partially sequencing from both ends. apba1-rab6b 1454635 n/a 1454643 unspecified role unspecified role neutral component neutral component n/a 1454647 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 1 apba1-rab6b-1 1454636 n/a 1454640 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1454648 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 8A app-q02410_2-1 1454637 n/a 1454644 unspecified role unspecified role neutral component neutral component c_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1454640 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 9B app-q02410_2-2 1454638 n/a 1454640 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1454644 unspecified role unspecified role neutral component neutral component c_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 9A app-q02410_2-3 1454639 n/a 1454642 unspecified role unspecified role neutral component neutral component Q9NRW1:p.Gln72Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1454644 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 9C and Movie S1