MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint conde-2013-1 Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Conde C., Osswald M., Barbosa J., Moutinho-Santos T., Pinheiro D., Guimarães S., Matos I., Maiato H., Sunkel C.E. cesunkel@ibmc.up.pt EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation q9vrq2_drome mad2 Dmel\CG17498 MAD2 Mad2 Dmel_CG17498 CG17498 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTAQATKNCITLKGSAQIIVEYLKYGINSILFQRGIYPAEDFNNTQQYGLTILMSKDPKIKTFLQNVLSQTEEWLSKNMINKISMVITNAHTKEVLECWDFNMQAELGDGDISDPTKATTTKELSRIQNEIRDVMRQISATVSYLPLLDCICTFDIMIHTLQNTELPAKWDETGAIVIQNPQAVQLRSFSTGLHKVDTVVNYKMST q24044_drome fzy-RA fzy Dmel_CG4274 BG:DS02740.14 CDC20 Cdc20 Cdc20[Fzy] Cdc20[fizzy] Dmel\CG4274 FZY Fizzy Fzy cdc20 dCDC20 dFZY fizzy CG4274 Cdc20FZY cdc2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSQFNFVSDLQNALIMDGETRGPAPRWKKKLEASLNGSVNTTRSVLSVSYNTSFSGVQAPTKTPGKSSEGKTKKSNTTPSKTPGGGDRFIPNRAATNFELAHFLVNKDSGDKSDEENDKATSSNSNESNVQASAHKGDRQKLISEVAQVGDSKGGRILCYQNKAPAAPETHNNPLKVVYSIKTPISTKSGSRYIPTTSERILDAPDFINDYYLNLMDWSADNIVAVALGSCVYLWNAQTGNIEQLTEFEEGDYAGSLSWIQEGQILAIGNSTGAVELWDCSKVKRLRVMDGHSARVGSLAWNSFLVSSGSRDGTIVHHDVRAREHKLSTLSGHTQEVCGLKWSTDFKYLASGGNDNLVNVWSAASGGVGTATDPLHKFNDHQAAVRALAWCPWQPSTLASGGGTADRCIKFWNVNNGTLMKSVDSKSQVCSLLFSRHYKELISAHGFANNQLTIWKYPTMVKQADLTGHTSRVLQMAMSPDGSTVISAGADETLRLWNCFAPDPLASKKAVSTSKGKQSVFRQSIR a1z6i7_drome BubR1 Bub1 13 31 BUB1 BUBR1 BcDNA:LD23835 CG 7838 DmBUB1 DmBubRI Dmbub1 Dmel\CG7838 bub1 bubR1 bubr1 dBUB1 l(2)k03113 l(2)k06109 mcl(2)Z1525 CG7838 Dmel_CG7838 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDFDNAKENIQPLASGRNVSLLQASLSQDSTHGQELLAQRKQMEEEVRTYKGADPLGAWYTFICWIEQSYPAGGSGSGLQTVLHQCLTKFEDDERYRQDKRLIKLFIKFMEKQKDKIEFYQQMYNNGIGTMLADFYIAWAYSYDLSGNMRKADEIFRLGLECRAEPLEDLKEAHHHFGYTVGQRMLYSTGEEANAVNQELNERRLALQSLHGRRQQISNSITVGSIRTGAAVKSGLPGVVQVEPSTTTSRRNVGRNVEVFNDENSEGNIPISNSETEVKSSLRSIIDSACSQENIKEPVAWNKANAKRHKHGKIFGSNASPDLGFDIHVDEQAMPPITNYERRLEQPFRFPTNFVAKNRPQEPWVTPVTIEDEPNASGLPCYNKCLLYPRPNIEFSPEEYRAYSFLKHRNPQHPFIHRNDDWWGTGRVIRGIRCYPNFARYSKPQDLDELDKFWKPPLVPGLQVVFDKIYNEVEQKEYQPEELLAAKWMEKRNVTVHGDFDMEETVCLPGNKMPRRKSFFPSSFRKSIMPRRVSSVQEEDEKKEDEATLIVKDIPSQVAPKIPESPCDQIDEPKIKIFEDSGSPRDNFAVPAVPVPKIEIYEDSEEPQPTPKTRPFYDGDETCSTQMFNMFIKSQAVSTPKGTQKQAPSRQFGTVLKELPLPEEAAHSVESPVETRSPTLRKQLSTILETSEHGTQSLATSGTTTKSTITSTSSPGSNAVSKLGSKIEENTPGQMRLQRVISAGVSAVLSEPEKPGGDNFLRRELWEPNAPSVPMLKSLRFQEDKTETIPRPLACFQEDKTETLPQMPIALPENSMDHGSLGAENCFSAPQLPTLEDESDLCGLFGKTPPKTNIFGSPKRNFDPTSQLFKSTQGDRRSFAVTGNKLEAAPSISNLQDSFMCDLSFVPETQPEAVAKSGAIQKFDIFLDDTQPKLTKSKPVALMVSSFDLDCTLPETQQVAIKEPGTQQENIGHSHMIASFMKDCTEIGSCPSQMPASVVKTNISSTSNKIKFSNDSMSESSRKAPVNENVGISKQPDEFFELNAATEMFATNISMIKNSTLLIPNAKVAEASELSIYYKTTPLTPKQSHRSWSQSDLETPPRENFVHPTSNGDQTVLNETLADANKNPFNVELISSLLESIDFSMYIEKLPHCQLVGHVKRLHPNTHLEVHNEKFEVSKMIGKGAYGSVYVGKHLKSGKKVALKQERPTNYWEFYICLEIHSRLTSEQMIPSYAHIDYALVGNNSSVYISEFSDYGSLIGVCNKVKSVTNRNMDEYVVMHLSCQMLDIVDHLHAMGIIHADIKPDNFLLMKPICADPNEVSLQLIDFGVSIDMKLFPDNQTFNYVHHDDLFKCIEMRTGRPWTYQLDLYGLVSVMHVLLFGRYMEVVQRSPSTIWMPKTNVPRYFQRTMWENIFRTLLNIRDCRTMPNLQQLRTQLKCALAEKEKYVAEAISKFNTILQK bubr1-fzy 1448171 n/a 1448172 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell n/a 1448173 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell n/a 1448174 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell association association false false false f6c fzy-bubr1 1448171 n/a 1448173 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell n/a 1448172 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell n/a 1448174 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell association association false false false f6c f7c cells overexpressing Mps1 and treated with colchicine, co-immunoprecipitated significantly higher levels of BubR1 with Cdc20 than non-induced control cells (Figure 7C) further supporting a model in which Mps1-regulated phosphorylation of BubR1 is instrumental for the formation of the Cdc20- BubR1 complex, which in turn is required for MCC assembly and sustained SAC function.