IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ betschinger-2013-2 Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Cell (0092-8674) 2013 Only protein-protein interactions imex curation betschinger-2013-3 Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Cell (0092-8674) 2013 Only protein-protein interactions imex curation betschinger-2013-1 Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Cell (0092-8674) 2013 Only protein-protein interactions imex curation fnip1_mouse Folliculin-interacting protein 1 Fnip1 Kiaa1961 protein protein mouse Mus musculus Mouse MAPTLFQKLFSKRSGLGAPGRDARDPDCAFSWPLPEFDPSQIRLIVYQDCERRGRNVLFDSSVKRKNEDTSVSKLCNDAQVKVFGKCCQLKPGGDSSSSLDSSITLSSDGKDQCPKYQGSRCSSDANMLGEMMFGSVAMSYKGSTLKIHQIRSPPQLMLSKVFTARTGSSICGSLNTLQDSLEFINQDSNTLKADSSTVSNGLLGNIGLSQFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNEDRDSGIARSASLSSLFITPFPSPNSSLTRSCASSYQRRWRRSQTTSLENGVFPRWSVEESFNLSDESCGPNPGIVRKKKIAIGVIFSLSKDEDENNKFNEFFFSHFPLFESHMNKLKSAIEQAMKMSRRSADASQRSLAYNRILDALTEFRTTICNLYTMPRIGEPVWLTMMSGTPEKNQLCHRFMKEFTFLMENASKNQFLPALITAVLTNHLAWVPTVMPNGQPPIKIFLEKHSSQSVGMLAKTHPYNPLWAQLGDLYGAIGSPVRLARTVVVGKRQDLVQRLLYFLTYFIRCSELQETHLLENGEDEAIVMPGTVITTTLEKGEIEESEYVLITMHRNKSSLLFKESEETRTPNCNCKYCSHPVLGQNTENVSQPEREDTQDNSKELLGISDECQKISPPDCQEENAVDVQQYRDKLRTCLDTKLETVVCTGSAPADKCVLSETCLEPREESWQNKELLDSDNHTGTAMRPTGIVVEKKPPDKNVPSAFSCEVTQTKVTFLIGDSMSPDSDTELRSQAVVDQINRHHSEPLKEDRGVADKHQESKITKDQSEDSDTQNIVSGESCELPCWSHSDPESMSLFDEYFNDDSIETRTIDDVPVKTSTDSKEYCCMLEYPKRLYTKTNKQKSELCKCIETVHQDSCNACFPQQDQRNSLSILVPHGDKESSDKKNAVGTEWDIPRNESSDSALGDSESEDTGPDIRRQAGGYCGGDQEDWTEEDEIPFPGSKLIEVSAVQPNIANFGRSLLGGYCSSYVPDFVLQGIGNDERLRQCLVSDLSHAVQHPVLDEPIAEAVCIIADMDKWTVQVASSQRRVTDNKLGKEVLVSSLVSNLLHSTLQLYKHNLSPNFCVMHLEDRLQELYFKSKMLSEYLRGQMRVHVKELGVVLGIESSDLPLLAAVASTHSPYVAQILL flcn_mouse Folliculin Flcn Bhd Birt-Hogg-Dube syndrome protein homolog protein protein mouse Mus musculus Mouse MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGAGSGDSPDQVEQAEEEEGGIQMSSRVRAHSPAEGASSESSSPGPKKSDMCEGCRSLAVGHPGYISHDKETSIKYVSHQHPNHPQLFSIVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIIAIMMDRIYLINSWPFLLGRIRGIISELQAKAFKVFEAEQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLADLEEESESWDNSEAEEEEKAPVTPEGAEGRELTSCPTESSFLSACGSWQPPKLTGFKSLRHMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVNLVHSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGLSPPVPIPAHVLASEFVVVVEVHTATRSNLHPAGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAALTNQNLSVDVVDQCLICLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSVLGASEEDNVKLLKFWMTGLSKTYKSHLMSTVRSPTATESRS err2_mouse_gene Mouse esrrb gene gene gene mouse Mus musculus Mouse tfe3_mouse Transcription factor E3 Tfe3 Tcfe3 protein protein mouse Mus musculus Mouse MSHAAEPARDAVEASAEGPRAVFLLLEERRPAESAQLLSLNSLLPESGIVADIELENILDPDSFYELKSQPLFLRSSLPISLQATPTTPATLSASSSAGGSRTPAMSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVIGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPSSAQPLPAPETAHATGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTAGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPNPGLLSLTTSSVSDSLKPEQLDIEEEGRPSTTFHVSGGPAQNAPPQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEGMVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES klf2_mouse_gene Lung krueppel-like factor gene gene gene mouse Mus musculus Mouse flcn-fnip1-1 1429092 n/a 1429095 unspecified role unspecified role prey prey n/a 1429096 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3D Interaction observed in both non-differentiated and differentiated cells. fnip1-flcn 1429092 n/a 1429095 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1429096 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3D Interaction observed in both non-differentiated and differentiated cells. tfe3-err2 1429093 n/a 1429097 unspecified role unspecified role prey prey cis regulatory elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1429098 unspecified role unspecified role bait bait association association false false false Fig. 6A tfe3-klf2 1429093 n/a 1429098 unspecified role unspecified role bait bait n/a 1429099 unspecified role unspecified role prey prey cis regulatory elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 6A flcn-fnip1 1429094 n/a 1429096 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1429095 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 3B