MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint farr_-2013-2 Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Farré J.C., Burkenroad A., Burnett S.F., Subramani S. ssubramani@ucsd.edu EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation farr_-2013-1 Phosphorylation of mitophagy and pexophagy receptors coordinates their interaction with Atg8 and Atg11. picpa Pichia pastoris (Yeast) Yeast Pichia pastoris anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Farré J.C., Burkenroad A., Burnett S.F., Subramani S. ssubramani@ucsd.edu EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation atg36_yeast Autophagy-related protein 36 ATG36 J0415 YJL185C protein protein yeast Saccharomyces cerevisiae Baker's yeast MASVNNYQVDCGSRSARIQPRINNGIHDEESLFEVLELSEEEFELDFHRLKSFNDVRVINNPDLSPECTNTAISRDETLESASSAFEVPSDEIAILSISSDSNKNSPPSEQPAPALRNIRSSSNSDRIDEWCLGSHLFNELHQNVPQSSDGVNHGFPVYSFKERELYTSAKLKKLTNAQRIAVQKLSRDLYPILRTCYREKTRRQLLTYHHERIFDDIPSFFPQRDFIFNYYSMPLEFDRLSDVDIDSSSRSRFTDESTGETLNRSPSAASSSLENTSWFGWTLLSRFLDREW 2F1499AFFF3CCB9C atg8_yeast Autophagy-related protein 8 ATG8 APG8 AUT7 YBL078C YBL0732 CVT5 Autophagy-related ubiquitin-like modifier ATG8 Cytoplasm to vacuole targeting protein 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFGR 1246CEFF5A038819 atg11_yeast Autophagy-related protein 11 Cytoplasm to vacuole targeting protein 9 ATG11 CVT9 YPR049C YP9499.07c protein protein yeast Saccharomyces cerevisiae Baker's yeast MADADEYSTAPTQQEITPLQTTATIINAISGECITTNVDFFVSLDKFKQFIARKWKIPPDQLLILLPYGNKLKPSMFKELLINRSFTLNDFYVYDRRLFSLVSKPTPTNLLTSKDSNPMNSPNSNDLTETLEYLIKNSHISQYQGSDTIMIKPMPSPLEDADVDLSRLNYHSVTSLLTTNLGWLSALEIDVHYFKSLIPDIIAHIKRIFDGLTVCSQYLKLYCFDVESLYNSNVQFLNQLVDNGMTSKWEKCFNDTLSKLTALEGDSLQKFINIESLLENEKSVKILNHSINGKLNKIKREIDENASFRDIITVNIDRLRQMFTPNESKFELEDQMAESFEVLVSEMRTRSRNVLDKEEEEFNSQEFLKSMNVMLEKDKKESVKTLFTISQALYSQIGELIDLKKSLQKHAVAILGNIAFTQMEILGIKRLLLNECNKDLELYKKYEVEFAQVEDLPLIYGLYLIEKYRRLSWFQQILSFISNFNQDLELFKQNELRTRNKWVKNFGSIATVFCEDLLSSSDFKRLNEYHSHTSPPNEDEEDENENSIANYRQDLVKVSQAIDNYMTQIKETDVSEPIIDLLSKTLFETKRFHIIYSNFKNNNNNSSNGNSISPEGSIALKSDDVVKGYKTRIKKLESLLHEFQYSDIGHWPQGVLNTHLKPFRGSATSINKKKFLGASVLLEPANISEVNIDSVSQANNHQIQELESNVDDLLHQLQLLKEENNRKSMQISEMGKKISDLEVEKTAYRETLTNLNQELARLTNEEQSHRTEIFTLNASFKKQLNDIISQDNEKIEKLTGDYDDVSKSRERLQMDLDESNKKHEQEVNLLKADIERLGKQIVTSEKSYAETNSSSMEKGEKFETIPLAEDPGRENQISAYTQTLQDRIFDIISTNIFILENIGLLLTFDNNNNIQIRRVKGLKKGTAQSNILDESTQMLDAHDNSLIKSPVFQKLKDEYELIKSVANGSEKDTQQSIFLGNITQLYDNKLYEVAVIRRFKDIETLAKKLTKENKIKRTLLERFQREKVTLRNFQIGDLALFLPTRENVNSVGSMSSSTSSLSSSFSSVDLSTPPPLDAMSIQSSPSVIHSNVINQASISGRDKNKLMRPWAAFTAFEESTRYFLKDEKGLTKGKEWFVGRIVTLEHFVADSPSNNPFRLPKGSVWFQVTAVVVSYQGV A904B92E9AE79449 atg32_yeast Autophagy-related protein 32 YIL146C ATG32 Extracellular mutant protein 37 ECM17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLEYQQREGKGSSSKSMPPDSSSTTIHTCSEAQTGEDKGLLDPHLSVLELLSKTGHSPSPMGQNLVTSIDISGNHNVNDSISGSWQAIQPLDLGASFIPERCSSQTTNGSILSSSDTSEEEQELLQAPAADIINIIKQGQEGANVVSPSHPFKQLQKIISLPLPGKEKTPFNEQDDDGDEDEAFEEDSVTITKSLTSSTNSFVMPKLSLTQKNPVFRLLILGRTGSSFYQSIPKEYQSLFELPKYHDSATFPQYTGIVIIFQELREMVSLLNRIVQYSQGKPVIPICQPGQVIQVKNVLKSFLRNKLVKLLFPPVVVTNKRDLKKMFQRLQDLSLEYGEDVNEEDNDDEAIHTKSRSYCRNKKAENSKKKSPKSNKKPKRKKQKFFTSWFTWGISITIGISFGCCVTYFVTAAYEHQTVKSLSLRPSILASLLSLDSSSDTINTPATASPSSTEQFLWFDKGTLQINFHSDGFIMKSLTIIKETWGKMNTFVLHALSKPLKFLENLNKSSEFSIDESNRILALGYILL pex3_picpa Peroxisomal biogenesis factor 3 PEX3 PAS2 Peroxin-3 Peroxisomal membrane protein PAS2 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MLEYTAGLIRRNKKKFLISSGIIGVGYYVTKTINNKIQEFQNRIREENFAKEQIKRRFHQTQSDCYMTFLSLLPVLCEPIMDDLPVETITKQLQIRRLEKQIGNKDVKNSGSTVLSDDFSTSQEGAISEDTNKPPELKSKNQLWQELKIKAITRFLTLIYCESLLIVFLHLQLNILSRKSYLETAIRLASETQGIDLVDQESNGDFSGNTQDENLSEQAFLSFSWWLLNKGWLEIKNKIEPCVEQHFGGINPRQQLKINEFAELLNKCQNCIDLKVLNLTEEDIHLGVGVIEDQSQPVGRKSTNFITNALLPPKEFEFFLLQQTNDLDFLSRFNNNIVNTESLNMLLDELNNYLNNADINLIVNKLATLGITKVLDEIVLNLLQKNRPNPISDMNIRDIDLNDFPPYKLAALLANITKQSISLTNNSVENPILADLNNLPELNDLSASVYSNFDP atg30_picpa Autophagy-related protein 30 ATG30 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MFSRKQVQKRNNELSSLHCSNSSNSLNRIHKNEETAKGTVGVNARGNNRSDNVASPGQLRPRTSSILTDNSEWILFSPENAEGEYVITSSDGIRRTNSNHYYYNYNEDDILSSSRRSSEDVYDAEQEYTEQPVNNHVQVEDEEDDDSIINDLTHVVDDYDYEEEDDKQDLTTRIDNWRKKQVSELLNELNHDDDLDPVLNRDKIDLIQSWGIENEKLNTKPRAKKRQRKSKRASFYGQDLLSKYSMEDLKIIKQIVAQLRDDLDKVKHDKPSSPLPNYHNTLKQAPSSNSQNPSFISYYSNYLTKNNSQQTPNSQSTSGSLLNNPNLEKYLPLFLKNLLYEDSNGSHQHPETSEKEHFWDNDLKSVNSSILTLSSNSKLKQEIL atg8_picpa Autophagy-related protein 8 ATG8 PAZ2 Autophagy-related ubiquitin-like modifier ATG8 Pexophagy zeocin-resistant mutant protein 2 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MRSQFKDEHPFEKRKAEAMRIRYKFADRIPVICEKVEKSDIPEVDKRKYLVPCDLTVGQFVYVIRKRIKIPSEKAIFIFIDDILPPTAALMSKVYEEHKDEDGFLYVLYSGENTFGDIPGVEEIE atg11_picpa Autophagy-related protein 11 ATG11 GSA9 PAZ6 Glucose-induced selective autophagy protein 9 Pexophagy zeocin-resistant mutant protein 6 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MDSSRHGLHPLQNSLLHQSQMDIYNSITGIRISAIPYNFNSYDQFKQYISASFGIAPADLFLLTAFGIKLKFSMIMNGDVREVYVFDRRFYDGQQMVDDKLDTALESLNQCEMLNMIKPMRSPLENADILRFVSYLKDITNRPNLSTEDLDLNKLRLVLNSLKRSSGWAAALLSDLKKTNYYKKVNEEVLCDNKKEIEIILISHNALIQYTNLMFKTLEKSFNESVDSLIMLQEQSLLENWKSYYQILKGVRFKGNYVLSDLLDEKMLENVAADSKALMGNVNDKLTRLRSRIDSEIISKRVMINDLYESLKKKYLDVPNLNSNSRTENDSDTLNRLTELVNQVVKDSKELPILDELLTTSGGNSTTLSAESVKKINVLVSVFETHSSTIIPQITELSNKLYDEKVEALNLKQDLQRTLLSDTIHKIVGVQLSILKATNLINNDLSKNISNLDFNELKMSIVKDLPLVFGLWLAGNLKKLKWLENFNKVAFKANEILEMLKFIESNYRSKWIDGFSKTNPCVNSNQGQRILALQINEDLKQQFVRDHLASSRIVLKTGSNVPTPGGSKGNSRAPTPEHDPHPPHLNAINKLLHNFNKDYNFGNLRQASEEVVRTHSPATKRDDRFGNQFWLTLIDNITTEDFYHYIDSLKENKVNIKVIKQLEKNLTDLGLGRIYETSNDKVISAGGGTIGPLNSQDTSYMNLLKKFLKNFEINDVTIQINISTLETENGNNNEKEKEPSFSNHELLQGYQRRVKKLESLLYQQSLHQSGPPPINMAYPPIRDYQQRSSANLMTSATATEMLMDTHARIHDTDMKQKRENVDEIQVLQTRIEQLQANLEQTSKERDDEREQKEILHLKLMKRDEEGDEDEKINGLVAANHQLQIRLEALQKQNQELQSLQERNNNEIHASQEREIEALKKQVVQLTEEKTQMSDEKDRLDLSNEHWKTQYEEAAMMKKDLLDNMTAQEQEYKNELNTHIKEVEDLKVKVENLEDEEANLIEIKENYESKLAQNESHFEELESIIKSLYGKLRLVIERTFQNVVTVCLMLEAIGLLMKRDEQYEENDPSNGIRIHRVKGLRSRRRSTTSKAASPLGNDEILELSSQIVAEADKQLVYFHQEPVKELESLETVLDFKFNQDFDKFTRLTYMDQKLLVESVTKRFKDVEQLARKLQKESNYSKTEIDGLIKEVNTRISIKDFKVGDLVLFLPTRDDTINMNMANTVEAVNRRASTVASFETYQPWAAFNVGAPHYFLINDVSKIDLNGRDWVLARIESMEEHKVTREGHRRNVGNPYNLNPDAVWYGVRAKEETVG atg8-atg36 1457447 n/a 1457449 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P46983:p.Ser31Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 1457450 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f4d f4f atg8-atg36-1 1457447 n/a 1457450 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457449 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P46983:p.[Phe33Ala;Leu36Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f4d f4f atg11-atg36 1457447 n/a 1457451 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457449 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell P46983:p.Ser97Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f4d f4f atg8-atg32 1457447 n/a 1457452 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P40458:p.[Ser81Ala;Ser83Ala;Ser85Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1457450 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false sf7 atg11-atg32 1457447 n/a 1457452 predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P40458:p.[Ser114Ala;Ser119Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1457451 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false sf7 atg30-atg8 1457448 n/a 1457453 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1457454 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457455 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false f1a atg30-pex3 1457448 n/a 1457454 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457453 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f1a atg8-atg30 1457448 n/a 1457454 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457455 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f1a atg30-atg11 1457448 n/a 1457454 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457453 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a 1457455 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1457456 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false f1f We validated independently the phosphorylation requirement for Atg30–Atg8 binding in P. pastoris by Atg30–haemagglutinin (HA) co-IP from different mutants cells in the presence or absence of phage l protein phosphatase. The interactions of both Atg8 and Atg11, but not the control Pex3, with Atg30 were severely affected by the phosphatase treatment. The AIM and S71A mutations in Atg30 also abolished the interaction with Atg8, but not with Atg11 or Pex3.