MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint rauf-2013-1 ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rauf M., Arif M., Dortay H., Matallana-Ramírez L.P., Waters M.T., Gil Nam H., Lim P.O., Mueller-Roeber B., Balazadeh S. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation rauf-2013-2 ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rauf M., Arif M., Dortay H., Matallana-Ramírez L.P., Waters M.T., Gil Nam H., Lim P.O., Mueller-Roeber B., Balazadeh S. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation rauf-2013-3 ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. arath-mesophyll Arabidopsis thaliana mesophyll mesophyll Mesophyll of plant leaf The photosynthetic parenchyma between the epidermal layers of a foliage leaf. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Rauf M., Arif M., Dortay H., Matallana-Ramírez L.P., Waters M.T., Gil Nam H., Lim P.O., Mueller-Roeber B., Balazadeh S. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation rauf-2013-4 ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Rauf M., Arif M., Dortay H., Matallana-Ramírez L.P., Waters M.T., Gil Nam H., Lim P.O., Mueller-Roeber B., Balazadeh S. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation rauf-2013-5 ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rauf M., Arif M., Dortay H., Matallana-Ramírez L.P., Waters M.T., Gil Nam H., Lim P.O., Mueller-Roeber B., Balazadeh S. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation nac92_arath NAC domain-containing protein 92 NAC92 NAC2 NAC6 ORE1 Protein ORESARA 1 MIJ24.11 At5g39610 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDYEASRIVEMVEDEEHIDLPPGFRFHPTDEELITHYLKPKVFNTFFSATAIGEVDLNKIEPWDLPWKAKMGEKEWYFFCVRDRKYPTGLRTNRATEAGYWKATGKDKEIFKGKSLVGMKKTLVFYKGRAPKGVKTNWVMHEYRLEGKYCIENLPQTAKNEWVICRVFQKRADGTKVPMSMLDPHINRMEPAGLPSLMDCSQRDSFTGSSSHVTCFSDQETEDKRLVHESKDGFGSLFYSDPLFLQDNYSLMKLLLDGQETQFSGKPFDGRDSSGTEELDCVWNF glk1_arath Transcription activator GLK1 GLK1 GPRI1 GBF'S PRO-RICH REGION-INTERACTING factor 1 Golden2-like protein 1 F23N11.11 At2g20570 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLALSPATRDGCDGASEFLDTSCGFTIINPEEEEEFPDFADHGDLLDIIDFDDIFGVAGDVLPDLEIDPEILSGDFSNHMNASSTITTTSDKTDSQGETTKGSSGKGEEVVSKRDDVAAETVTYDGDSDRKRKYSSSASSKNNRISNNEGKRKVKVDWTPELHRRFVEAVEQLGVDKAVPSRILELMGVHCLTRHNVASHLQKYRSHRKHLLAREAEAANWTRKRHIYGVDTGANLNGRTKNGWLAPAPTLGFPPPPPVAVAPPPVHHHHFRPLHVWGHPTVDQSIMPHVWPKHLPPPSTAMPNPPFWVSDSPYWHPMHNGTTPYLPTVATRFRAPPVAGIPHALPPHHTMYKPNLGFGGARPPVDLHPSKESVDAAIGDVLTRPWLPLPLGLNPPAVDGVMTELHRHGVSEVPPTASCA glk2_arath Transcription activator GLK2 GLK2 GPRI2 GBF'S PRO-RICH REGION-INTERACTING factor 2 Golden2-like protein 2 MLN1.11 At5g44190 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLTVSPAPVLIGNNSKDTYMAADFADFTTEDLPDFTTVGDFSDDLLDGIDYYDDLFIGFDGDDVLPDLEIDSEILGEYSGSGRDEEQEMEGNTSTASETSERDVGVCKQEGGGGGDGGFRDKTVRRGKRKGKKSKDCLSDENDIKKKPKVDWTPELHRKFVQAVEQLGVDKAVPSRILEIMNVKSLTRHNVASHLQKYRSHRKHLLAREAEAASWNLRRHATVAVPGVGGGGKKPWTAPALGYPPHVAPMHHGHFRPLHVWGHPTWPKHKPNTPASAHRTYPMPAIAAAPASWPGHPPYWHQQPLYPQGYGMASSNHSSIGVPTRQLGPTNPPIDIHPSNESIDAAIGDVISKPWLPLPLGLKPPSVDGVMTELQRQGVSNVPPLP nac6-glk1 1420680 n/a ORE1 1420685 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag dna binding domain dna binding domain tag dna binding domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false n/a Glk1 1420686 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag activation domain activation domain tag activation domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f1a nac6-glk2 1420680 n/a ORE1 1420685 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag dna binding domain dna binding domain tag dna binding domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false n/a Glk2 1420687 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag activation domain activation domain tag activation domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false 1420700 1420697 physical association physical association false false false f1a glk2-nac6 1420680 n/a Glk2 1420687 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false tag activation domain activation domain tag activation domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a ORE1 1420685 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag dna binding domain dna binding domain tag dna binding domain predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false 1420707 1420703 physical association physical association false false false f1a nac6-glk2-1 1420681 n/a ORE1 1420685 unspecified role unspecified role bait bait purified purified tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a Glk2 1420687 unspecified role unspecified role prey prey purified purified tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false f1b glk2-nac6-1 1420682 n/a ORE1 1420685 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tag nyfp nYFP predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Glk2 1420687 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed tag cyfp cYFP predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false F1C glk2-nac6-2 1420683 n/a ORE1 1420685 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a Glk2 1420687 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f2e nac6-glk2-2 1420684 n/a Glk2 1420687 unspecified role unspecified role prey prey living cell living cell n/a ORE1 1420685 unspecified role unspecified role bait bait living cell living cell range binding region binding-associated region binding region predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false text