IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ vinayagam-2013-1 Protein complex-based analysis framework for high-throughput data sets. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Vinayagam A., Hu Y., Kulkarni M., Roesel C., Sopko R., Mohr S.E., Perrimon N. vinu@genetics.med.harvard.edu, perrimon@receptor.med.harvard.edu Sci Signal 2013 Only protein-protein interactions imex curation q9w384_drome Bap111 DALAO dalao Dmel_CG7055 BAF57 BAP 111 BAP111 Dalao Dmel\CG7055 anon-EST:Liang-1.83 clone 1.83 CG7055 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALPSNYKQIAVGGQGSATPLQGGGGGSGGGSRSRSSGGGGGGDRNKDQTPIFTHSNYGNPAFTPQKVTKSSSSKNQNESRLPKPPKPPEKPILPYMRYSKRVWDSVKAKHPELKLWELGKKIGAMWKLLPEDEKTEFIDEYEAEKLEYEKSLKAYHQTPAYQAYMSAKSKVKTDVDMHETPSRGGGSKSQHERRIDIQPAEDEDDQDEGYTTKHLAYARYLHNHRLINEIFSEAVVPDVRSVVTTTRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDEETFQKMVLRMYEDIKRDRQRLDEPNANANSAANPAAAAATAAAAPVTRSEEAVKPPTQPGQPAATPAGQEPASAVPAPAAPPKETPPAVKPATLNPTPSSTPTPAPAVHVHETASKTDPEPMDIEPPPKPSVPPPPIKPEKLEMAAALPPQSTLVEPPKTEPAKVVAQPGKVPTPVPTPTPPPEVAPAAGAATAATTTTTTTPTPPPVPQQVPLPQQQGGPAPPPGMPQMHPHPGHPPPGHSLMPPHMGPHQPPPGMPGLPPPPPHTGYANYGGPPHGPPPGPPGGPARPYYQPQYGGHPTPQPYYAPFSPYQQSYGPPPGSHYMSPRPPPPQHNGNPGHPYAPEHGSNPPPPQQQQQQQPPPGHLHEPSGGGPGAPGGGAGAAAAAAPGAGVYPTPGAGAGPGAPPGPAGGAPLGEAAVAGGVAPPAATAPGKHPEAEKHEAD q9vr61_drome Non-specific serine/threonine protein kinase S6kII Dmel\CG17596 RSK Rsk S6KII dRSK ign rsk rsk2 Dmel_CG17596 CG17596 D-RSK dRsk protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPLADSQKDLRQQPQQQQQQQHVSSTSSSNNAEQQCSSSGLGLQLRQRMQITSSGCSSLAVTPMEHTPTEDDESGGGNSGVTSSVTTVTSSQRRQQLQQVQQQSALQAALEQHHISPTADLDSARKRPTHRTLCPPPELMELSDSESQGGVETGGRREGATGRSAPDLEDTEPLYETENEFELKEVIKEGHDKADPSQFELLRVLGEGSFGKVFLVRKIIGKDAGTLYAMKVLKKATLKVKDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGIIYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHGQTRQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFATIDWVRLERKQVRPPFIPAVSRDDAFYFDVEYTSKSPRDSPGGPISASAHEIFRGFSFVAPVLLEGQCAGSNMCSTSASPLHSIAPIPAAPVGAPRTLPGVLPGNFHAEYNLLQELGRGTFSVCRLCEHRASKKHYAVKVIEKAAVAAASTSTSADCWEEVEIMLRYGNHPNIVTLYSVYEDAGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFAKQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFASTPNDSPDVILKRIGSGQIDFTSSRWALISVPAKELLRQMLHIVPENRPTAARILEHDWLREQFAGGVQLTEYAVAPGSQLSLGAQQQQQNHISMALRGAVDATFRAIAIPQAANVGPVELSMLAKRRAKDRANLHS dalao-s6kii-1 1385113 n/a 1385114 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1385115 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4b