IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ erce-2013-1 A conditional two-hybrid (C2H) system for the detection of protein-protein interactions that are mediated by post-translational modification. ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Erce M.A., Low J.K., Hart-Smith G., Wilkins M.R. A conditional two-hybrid (C2H) system where an interaction between two proteins of interest can be tested with and without the condition of a PTM or additional partner proteins. marc.wilkins@unsw.edu.au Proteomics (1615-9853) 2013 Only protein-protein interactions imex curation erce-2013-2 A conditional two-hybrid (C2H) system for the detection of protein-protein interactions that are mediated by post-translational modification. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay antibody detection identification by antibody antibody detection Erce M.A., Low J.K., Hart-Smith G., Wilkins M.R. A conditional two-hybrid (C2H) system where an interaction between two proteins of interest can be tested with and without the condition of a PTM or additional partner proteins. marc.wilkins@unsw.edu.au Proteomics (1615-9853) 2013 Only protein-protein interactions imex curation gcn4_yeast General control transcription factor GCN4 ARG9 GCN4 AAS3 Amino acid biosynthesis regulatory protein AAS101 General control protein GCN4 YEL009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER 2ED1B8E35D509578 nop3_yeast Nucleolar protein 3 MTR13 MTS1 Mitochondrial targeting suppressor 1 protein Nuclear polyadenylated RNA-binding protein 1 NPL3 NAB1 YDR432W D9461.19 NOP3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEAQETHVEQLPESVVDAPVEEQHQEPPQAPDAPQEPQVPQESAPQESAPQEPPAPQEQNDVPPPSNAPIYEGEESHSVQDYQEAHQHHQPPEPQPYYPPPPPGEHMHGRPPMHHRQEGELSNTRLFVRPFPLDVQESELNEIFGPFGPMKEVKILNGFAFVEFEEAESAAKAIEEVHGKSFANQPLEVVYSKLPAKRYRITMKNLPEGCSWQDLKDLARENSLETTFSSVNTRDFDGTGALEFPSEEILVEALERLNNIEFRGSVITVERDDNPPPIRRSNRGGFRGRGGFRGGFRGGFRGGFSRGGFGGPRGGFGGPRGGYGGYSRGGYGGYSRGGYGGSRGGYDSPRGGYDSPRGGYSRGGYGGPRNDYGPPRGSYGGSRGGYDGPRGDYGPPRDAYRTRDAPRERSPTR 024439E4B6578787 gcn4-gcn4-3 1391877 n/a 1391879 unspecified role unspecified role prey prey t18-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1391879 unspecified role unspecified role bait bait t25-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3a and 3b npl3-npl3-1 1391877 n/a 1391880 unspecified role unspecified role prey prey s-t18 tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1391880 unspecified role unspecified role bait bait t25-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 3a and 3b Co-expression with active methylase Hmt1 increases the binding of Npl3. The effect was not seen using an inactive mutant version of Hmt1. npl3-npl3 1391878 n/a 1391880 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) n/a 1391880 unspecified role unspecified role bait bait purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3c Methylated Npl3 was found to have increased self-association.