UniProt UniProt http://www.uniprot.org jonas-2013-2 An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation jonas-2013-3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation jonas-2013-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation jonas-2013-1 An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation g5ef47_caeel smg-7 CELE_Y73B6A.4 Y73B6A.4 protein protein caeel Caenorhabditis elegans MSDEWEQLTVELRKIPRGTEAAPQYLRHLMKMFVADFETAVSKRFDVKFWNKLKSMMDEITKAMENDRLVNHNVQNLAIGFLTDLSLLVHYHYEIPNYGNDISKQLTWTPDVFLNRKPIKSKKNSRVFMAYVLLRMGDLMRYKENYPKAQEYYEQSCRINPADGAVWNQLGLISSLGAKNLESVYFHTRALHATMEFPTASGGLTNIFKNFANRDISRPMPIKDLYLSCLGRIHFLLEIEDSSVHLQKIGEEAATSKEMIVPLMSVYKHLEDGTELEQRAVEYVKTIWCTAYRSLLKTLDDYKEESKKLADVPHLLHILALLLCAPKLLRGIEDQTEDEVTSICEWLLCANCDEKIKDSDAFGYFHCLQRIQYPLTRTQLAQKLVEIEDEDESKDSDQSTIPEEDQKSLSTDTSRIQNEDSEPESESESSDEEVLQRGRRRGRNPNLDESVETDEDEF g5ecf1_caeel smg-5 CELE_W02D3.8 W02D3.8 protein protein caeel Caenorhabditis elegans MQKSDEVTEKFKRYCNQLEKYGQTENVHSPVMAMLRRKGRKQLIEIMKRDGDCTSSINKLWIVGYYHPFQFFIRDKEKNMAIAVLLTMFCGELQEMLSLPDDKYPALWNMYIGDFHRYMPDEEIQKCLAVGYYSRAIDLDPNQGRAFHVLAGLRADLNVAQKLRLMILGQLADAPYKKGTELLEYLKFPQKESTDKLMVDFVIWALNEKSKRMDYQMTGIKIVNEFKAEIEQKLEFDWSLIMSTCRLASKLAMKKFGFQQFYNCFDTISTLYITIYSRTISSKCLLAEAISWISDSAEILGHLDEQKNEPHFQKLSVFAKTKWNELNDLVMNHINSVFTSMSLTINPSISMTSFLLNGPISEPNVEFLSQLINYLVSVEFPPMEIIHDREESGPLLRRINQSEQKRLDIQIKTQNDEVNREDWRPVYVLMDYDVIVDKIRIALKIWDIDDFICILPSTVLDELDYQKTKNRAVRPVIRALMELQAEGKIVLKKCDNERSCAEQLVQSARRSAEDHKHIVAFLCKNPSEQKEMEGVTFYDIDQFYMKYLE smg7_human Protein SMG7 SMG-7 homolog EST1-like protein C SMG7 KIAA0250 C1orf16 EST1C protein protein human Homo sapiens Human MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH smg5_human Protein SMG5 SMG5 EST1B KIAA1089 EST1-like protein B LPTS-RP1 LPTS-interacting protein SMG-5 homolog protein protein human Homo sapiens Human MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG rent1_human Regulator of nonsense transcripts 1 UPF1 RENT1 KIAA0221 ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog protein protein human Homo sapiens Human MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY smg_5-smg_7-1 1432061 n/a 1432065 unspecified role unspecified role prey prey g80 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false v5 v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432066 unspecified role unspecified role bait bait q94 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ha ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false h116 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false g91 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 3B. smg5-smg7-2 1432061 n/a 1432067 unspecified role unspecified role prey prey Q92540:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q92540:p.Thr103Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432068 unspecified role unspecified role bait bait gly120glu required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 3C, 6B smg5-smg7-1 1432061 n/a 1432067 unspecified role unspecified role prey prey yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432068 unspecified role unspecified role bait bait Q9UPR3:p.Gly120Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6A. smg7-upf1-1 1432061 n/a 1432067 unspecified role unspecified role bait bait k54 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false r141 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false y142 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1432069 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 6A. smg5-smg7-3 1432061 N/A 1432067 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1432069 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1432068 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 6A smg5-upf1-1 1432062 N/A 1432067 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432069 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432068 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Figure 6B smg7-smg5-1 1432062 n/a 1432068 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1432069 unspecified role unspecified role prey prey n/a 1432067 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q92540:p.Arg163Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false Q92540:p.Lys66Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false association association false false false Figure 6C. upf1-smg5-3 1432062 n/a 1432068 unspecified role unspecified role prey prey n/a 1432069 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1432067 unspecified role unspecified role prey prey association association false false false Figure 6D smg_5-smg_7-5 1432063 N/A 1432066 unspecified role unspecified role neutral component neutral component gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag exp feature detect experimental feature detection exp feature detect n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 1432065 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag exp feature detect experimental feature detection exp feature detect undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3A Rworking 0.222 Rfree 0.249 Supp. Table 1 smg_5-smg_7-4 1432064 n/a 1432066 unspecified role unspecified role neutral component neutral component gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag exp feature detect experimental feature detection exp feature detect n-term range n-terminal range n-term range n-term range n-terminal range n-term range false G5ECF1:p.Gly91Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1432065 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag exp feature detect experimental feature detection exp feature detect undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false G5EF47:p.Gly80Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 3A Rworking 0.222 Rfree 0.249 Fig. 3a