UniProt UniProt http://www.uniprot.org kaur-2013-2 Arabidopsis RING peroxins are E3 ubiquitin ligases that interact with two homologous ubiquitin receptor proteins(F). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kaur N., Zhao Q., Xie Q., Hu J. In figure 4 there is contradictory info given as to which construct is the Bait/Prey:-Material and methods section states "PEX2, PEX10, PEX12 etc.....cloned into the pGILDA bait vector." but the text states "DSK2s (bait) and other RING proteins (prey)". These Baits/Preys have been entered as "unspecified". Interactions involving pLexA-53 and pB42AD-T plasmids (positive controls) have not been entered as the origins of these constructs are not known. huji@msu.edu Arabidopsis cDNA seedling library J Integr Plant Biol (1672-9072) 2013 Only protein-protein interactions imex curation kaur-2013-3 Arabidopsis RING peroxins are E3 ubiquitin ligases that interact with two homologous ubiquitin receptor proteins(F). in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kaur N., Zhao Q., Xie Q., Hu J. huji@msu.edu Arabidopsis cDNA seedling library J Integr Plant Biol (1672-9072) 2013 Only protein-protein interactions imex curation kaur-2013-1 Arabidopsis RING peroxins are E3 ubiquitin ligases that interact with two homologous ubiquitin receptor proteins(F). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kaur N., Zhao Q., Xie Q., Hu J. huji@msu.edu Arabidopsis cDNA seedling library J Integr Plant Biol (1672-9072) 2013 Only protein-protein interactions imex curation dsk2a_arath Ubiquitin domain-containing protein DSK2a DSK2A T23A1.5 At2g17190 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGGEADSRQPLTAEGVAVAVNVRCSNGTKFSVTTSLDSTVESFKELIAQNSDVPANQQRLIYKGRILKDDQTLLSYGLQADHTVHMVRGFVPSSPSAPAANAGNQTTAPQAVGSNDSSNLGGGESLFPGLGFNPLGGGNAMAGLFGSGLPDLEQAQQQLAQNPNMIREMMNTPAIQNLMNNPEFMRSMIMNNPQMRELVDRNPELGHVLNDPSILRQTLEAARNPELMREMMRNTDRAMSNIESMPEGFNMLRRMYENVQEPLMNATTMSENAGNNTSSNPFAALLGNQGVTTQGSDTSNNISAPNAETGTPNANPLPNPWGATAGQTTAPGRTNAGLGGLGGLGGLGGLGMLGADSPLGATPDASQLSQILQNPAMSQMMQSVLSNPQYMNQLMSLNPQLRSMLDMNPQLREMMQNPDFLRQFSSPEMMQQMMSLQQSLFSQNRNTAGQDPTQTGAATGTANNGGLDLLMNMFGSLGAGGLSGTNQPNVPPEERFATQLQQLQEMGFYDRAENIRALLATNGNVNAAVERLLGSIGQ pex2_arath Peroxisome biogenesis protein 2 PEX2 TED3 E3 ubiquitin-protein ligase PEX2 Peroxin-2 Pex2p F19K16.23 F20B17.23 At1g79810 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTPSTPADDAWIRSYQRLLPESQSLLASRRSVIPVAISRVNQFDAARLDVEMSAMLKEQLVKVFTLMKPGMLFQYEPELDAFLEFLIWRFSIWVDKPTPGNALMNLRYRDERGVVAQHLGKVRTGLEGPGLTSPQKIWYCVASVGGQYLFSRLQSFSAFRRWGDSEQRPLARRLWTLVQRIEGIYKAASFLNLLSFLYTGRYRNLIEKALKARLVYRSPHMNRSVSFEYMNRQLVWNEFSEMLLLLLPLLNSSAVKNILSPFAKDKSSSTKEDTVTCPICQVDPAIPFIALPCQHRYCYYCIRTRCASAASFRCLRCNEPVVAIQREGVSSGK dsk2b_arath Ubiquitin domain-containing protein DSK2b DSK2B T23A1.6 At2g17200 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGGEGDSSQPQSGEGEAVAVNIRCSNGTKFSVKTSLDSTVESFKELVAQSSDVPANQQRLIYKGRILKDDQTLLSYGLQADHTIHMVRGSAPSSAPPPAPAASQTTAPSVTRGVGSDNSSNLGGASPGESLFPGLGFNPLGGGNAMSGLFGAGLPDLVQTQQQLAQNPNMIRDMMNTPAIQNLMNNPEFMRSMIMNNPQMRELVDRNPELGHVLNDPSILRQTLEAARNPELMREMMRNTDRAMSNIESMPEGFNMLRRMYENVQEPLMNATTMSGNAGNNTGSNPFAALLGNQGVTTQGSDASNNSSTPNAGTGTIPNANPLPNPWGATGGQTTAPGRTNVGGDARSPGLGGLGGLGSLGGLGGLGMLGADSPLGATPDASQLSQLLQNPAISQMMQSVFSNPQYMNQLMSLNPQLRSMLDSNPQLREMMQNPDFLRQFSSPEMMQQMMTLQQSLSQNRNTASQDAGQTGAATGNNGGLDLLMNMFGSLGAGGLSGTNQSNVPPEERYATQLQQLQEMGFYDRAENIRALLATNGNVNAAVERLLGSIGQ pex12_arath Peroxisome biogenesis protein 12 At3g04460 T27C4.11 Peroxin-12 PEX12 APM4 Pex12p Protein ABERRANT PEROXISOME MORPHOLOGY 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLFQVGGEGTRPTFFEMAAAQQLPASLRAALTYSLGVFALRRSFLHKILDYEDEFFAALMLILEGHSLRTTDGSFAESLYGLRRKSARLRLRKDSARKDSSEEVQHSGLEKRQRILSVVFLVVLPYFKSKLHAIYNKEREARLRESLWGAEDQGFDEADFFTGDDSIVSREPSGNEELSVRVQLATKIKKFIAVCYPWIHASSEGLSFTYQLLYLLDATGFYSLGLQALGIQVCRATGQELMDTSSRISKIRNHERERLRGPPWLKTVQGALLSCSYAVLDYAQTGLIAAVFIFKMMEWWYQSAEERLSAPTVYPPPPPPPAPKMAKEGIPLPPDRSLCALCLQKRANPSVVTVSGFVFCYSCVFKYVSKYKRCPVTLIPASVDQIRRLFQDT pex10_arath Peroxisome biogenesis factor 10 PEX10 At2g26350 T9J22.2 Pex10p PER10 Peroxin-10 Peroxisome assembly protein 10 Peroxisomal biogenesis factor 10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRLNGDSGPGQDEPGSSGFHGGIRRFPLAAQPEIMRAAEKDDQYASFIHEACRDAFRHLFGTRIALAYQKEMKLLGQMLYYVLTTGSGQQTLGEEYCDIIQVAGPYGLSPTPARRALFILYQTAVPYIAERISTRAATQAVTFDESDEFFGDSHIHSPRMIDLPSSSQVETSTSVVSRLNDRLMRSWHRAIQRWPVVLPVAREVLQLVLRANLMLFYFEGFYYHISKRASGVRYVFIGKQLNQRPRYQILGVFLLIQLCILAAEGLRRSNLSSITSSIQQASIGSYQTSGGRGLPVLNEEGNLITSEAEKGNWSTSDSTSTEAVGKCTLCLSTRQHPTATPCGHVFCWSCIMEWCNEKQECPLCRTPNTHSSLVCLYHSDF dsk2a-pex2 1420473 n/a 1420476 unspecified role unspecified role bait bait n/a 1420477 unspecified role unspecified role prey prey ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3B dsk2b-pex2 1420473 n/a 1420477 unspecified role unspecified role prey prey ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420478 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3B dsk2b-pex2 1420473 n/a 1420477 unspecified role unspecified role unspecified role unspecified role ring sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420478 unspecified role unspecified role unspecified role unspecified role region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4A dsk2a-pex2 1420473 n/a 1420476 unspecified role unspecified role unspecified role unspecified role region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420477 unspecified role unspecified role unspecified role unspecified role ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4A dsk2a-pex12 1420473 n/a 1420476 unspecified role unspecified role unspecified role unspecified role n/a 1420479 unspecified role unspecified role unspecified role unspecified role ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4B dsk2b-pex12 1420473 n/a 1420478 unspecified role unspecified role unspecified role unspecified role n/a 1420479 unspecified role unspecified role unspecified role unspecified role ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4B dsk2a-pex10 1420473 n/a 1420480 unspecified role unspecified role unspecified role unspecified role ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420476 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 4B weak interaction dsk2b-pex10 1420473 n/a 1420480 unspecified role unspecified role unspecified role unspecified role ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420478 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 4B weak interaction dsk2a-pex2-1 1420474 n/a 1420476 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1420477 unspecified role unspecified role prey prey ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3C dsk2b-pex2-1 1420474 n/a 1420478 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1420477 unspecified role unspecified role prey prey ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3C pex2-dsk2b 1420475 n/a 1420477 unspecified role unspecified role bait bait ring_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1420478 unspecified role unspecified role prey prey physical association physical association false false false none