mbinfo MBInfo http://www.mechanobio.info/ http://www.mechanobio.info/ lawson-2012-1 FAK promotes recruitment of talin to nascent adhesions to control cell motility. human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation lawson-2012-2 FAK promotes recruitment of talin to nascent adhesions to control cell motility. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down western blot western blot Immuno blot Immuno blot Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation lawson-2012-3 FAK promotes recruitment of talin to nascent adhesions to control cell motility. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Lawson C., Lim S.T., Uryu S., Chen X.L., Calderwood D.A., Schlaepfer D.D. Participant detection: The colocalization detected by fluorescence patterns were then quantitated on a pixel-by-pixel basis and the Pearson's correlation coefficient was calculated. Some of the coimmunoprecipitations (Figs 1C, 2B, 3A, 5D) and colocalization (Figs 2C, 2D) experiments could not be curated since the specific talin isoform involved in these interactions was not specified and could not be identified. Coimmunoprecipitations (Fig 4A) and colocalizations (Fig 4B, 4C, S2A, S3A, S3B, S3C, S3D) involving GFP-FAK could not be curated since the source of GFP-FAK could not be identified. Direct binding assay (Fig 3E, S2C, S2D) could not be curated since the source of GST-talin could not be identified. dschlaepfer@ucsd.edu J. Cell Biol. (0021-9525) 2012 Only protein-protein interactions imex curation fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC tln1_chick Talin-1 TLN1 TLN protein protein chick Gallus gallus (Chicken) Chicken MVALSLKISIGNVVKTMQFEPSTMVYDACRMIRERVPEAQMGQPNDFGLFLSDEDPKKGIWLEAGKALDYYMLRNGDTMEYKKKQRPLKIRMLDGTVKTVMVDDSKTVTDMLTTICARIGITNYDEYSLVREIMEEKKEEVTGTLKKDKTLLRDEKKMEKLKQKLHTDDELNWLDHGRTLREQGIDDNETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGYQCQIQFGPHNEQKHKPGFLELKDFLPKEYIKQKGERKIFMAHKNCGNMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQFNRVGKAELGSVALPAIMRTGAGGPENFQVGTMPQAQMQITSGQMHRGHMPPLTSAQQALTGTINSSMQAVNAAQATLDDFETLPPLGQDAASKAWRKNKMDESKHEIHSQADAITAGTASVVNLTAGDPADTDYTAVGCAVTTISSNLTEMSKGVKLLAALMEDEGGNGRQLLQAAKNLASAVSDLLKTAQPASAEPRQNLLQAAGLVGQTSGELLQQIGESDTDPRFQDMLMQLAKAVASAAAALVLKAKNVAQKTEDSALQTQVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLIEAGKLVAKSAEGCVEASKAATNDDQLLKQVGVAATAVTQALNDLLQHIKQHATGGQPIGRYDQATDTILNVTENIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGETDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLREAAEGLRMATNAAAQNAIKKKLVHKLEHAAKQAAASATQTIAAAQHAAASNKNPAAQQQLVQSCKVVADQIPMLVQGVRGSQSQPDSPSAQLALIAASQNFLQPGGKMVAAAKATVPTITDQASAMQLSQCAKNLAAALAELRTAAQKAQEACGPLEIDSALGLVQSLERDLKEAKAAARDGKLKPLPGETMEKCAQDLGNSTKAVTSAIAHLLGEVAQGNENYTGIAAREVAQALRSLSQAARGVAANSSDPQAQNAMLECASDVMDKANNLIEEARKAVAKPGDPDSQQRLVQVAKAVSQALNRCVNCLPGQRDVDAAIRMVGEASKRLLSDSFPPSNKTFQEAQSQLNRAAAGLNQSANELVQASRGTPQDLAKSSGKFGQDFNEFLQAGVEMASLSPTKEDQAQVVSNLKSISMSSSKLLLAAKALSADPTSPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRELETVKELLENPTQTVNDMSYFSCLDSVMENSKVLGESMAGISQNAKNSKLPEFGESISAASKALCGLTEAAAQAAYLVGVSDPNSQAGQQGLVDPTQFARANQAIQMACQNLVDPACTQSQVLSAATIVAKHTSALCNTCRLASSRTANPVAKRQFVQPAKEVANSTANLVKTIKALDGAFNEENRERCRAATAPLIEAVDNLTAFASNPEFATVPAQISPEGRRAMEPIVTSAKTMLESSAGLIQTARSLAVNPKDPPQWSVLAGHSRTVSDSIKKLITNMRDKAPGQRECDEAIDVLNRCMREVDQASLAAISQQLAPREGISQEALHNQMITAVQEINNLIEPVASAARAEASQLGHKVSQMAQYFEPLILAAIGAASKTPNHQQQMNLLDQTKTLAESALQMLYTAKEAGGNPKQAAHTQEALEEAVQMMKEAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGEPEGTFVDYQTTMVKTAKAIAVTVQEMVTKSTTNPDELGILANQLTNDYGQLAQQAKPAALTAENEEIGSHIKRRVQELGHGCAALVTKAGALQCSPSDAYTKKELIESARKVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNRENSETFADHREGILKTAKALVEDTKVLVQNATASQEKLAQAAQSSVSTITRLAEVVKLGAASLGSEDPETQVVLINAVKDVAKALGDLIGATKAAAGKAGDDPAVYQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATIEHIRQELAVFSSPVPPAQVSTPEDFIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVSADVRQRALRFGKECADGYLELLEHVLVILQKPTHELKQQLAGYSKRVASSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGVIPANAVDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASEEKLISSAKQVAASTAQLLVACKVKADHDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFQDHDETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAMIRQQQYKFLPTELRDEEQN ptk2-pxn-7 1383761 n/a 1383764 unspecified role unspecified role neutral component neutral component n/a 1383765 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 1F, 1G. FAK colocalization with paxillin was unaffected in HUVEC cells knocked down for talin. ptk2-pxn-9 1383762 n/a 1383764 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1383765 unspecified role unspecified role prey prey physical association physical association false false false Fig 3A (middle panel). ptk2-tln1-1 1383763 n/a 1383766 unspecified role unspecified role prey prey ferm f2-f3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P54939:p.Trp359Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P54939:p.Ala360Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 1383764 unspecified role unspecified role bait bait Q05397:p.Glu1015Ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1383783 1383777 physical association physical association false false false Fig 3B, 3C, S2F.