IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ angeles-2011-5 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-2 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip tag visualisation tag visualisation Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-3 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-4 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. human-brain-cortex Homo sapiens brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-1 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg human brain cDNA library Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-6 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. in vitro In vitro oxidoreduct assay oxidoreductase assay oxidoreduct assay predetermined predetermined participant predetermined Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-7 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. angeles-2011-8 Mutations in LRRK2 increase phosphorylation of peroxiredoxin 3 exacerbating oxidative stress-induced neuronal death. human-chp212 Human CHP212 Neuroblastoma cell line chp212 Human neuroblastoma cell line https://www.lgcstandards-atcc.org/products/all/CRL-2273 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Angeles D.C., Gan B.H., Onstead L., Zhao Y., Lim K.L., Dachsel J., Melrose H., Farrer M., Wszolek Z.K., Dickson D.W., Tan E.K. Parkinsons - Interactions investigated in the context of Parkinsons disease LRRK2 constructs received from matthew j farrer have been entered as Human in origin, -as used in his previous work. The construct pSG5-PRDX3 (contains PRDX3) is murine in origin - as shown by previous publications by Chi V. Dang ( see pmid:12011429). PRDX3 cDNA was shown to be Human in origin by Blatting, and PRDX3 as used in Kinase assays was Human as shown by mutation mapping. gnrtek@sgh.com.sg Hum. Mutat. (1059-7794) 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. prdx3_human Thioredoxin-dependent peroxide reductase, mitochondrial Peroxiredoxin-3 Peroxiredoxin III Antioxidant protein 1 Protein MER5 homolog HBC189 PRDX3 AOP1 Thioredoxin-dependent peroxiredoxin 3 protein protein human Homo sapiens Human MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE hydrogen peroxide hydrogen peroxide [OH(OH)] dihydrogen dioxide H2O2 HOOH Hydrogen peroxide HYDROGEN PEROXIDE Oxydol bis(hydridooxygen)(O--O) dihydrogen peroxide dihydrogen(peroxide) dioxidane hydrogen peroxide small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) MHAJPDPJQMAIIY-UHFFFAOYSA-N prdx3_mouse Thioredoxin-dependent peroxide reductase, mitochondrial Prdx3 Aop1 Mer5 Perioredoxin-3 PRX III Antioxidant protein 1 Protein MER5 Thioredoxin-dependent peroxiredoxin 3 protein protein mouse Mus musculus Mouse MAAAAGRLLWSSVARHASAISRSISASTVLRPVASRRTCLTDILWSASAQGKSAFSTSSSFHTPAVTQHAPYFKGTAVVNGEFKELSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNITLLSDITKQISRDYGVLLESAGIALRGLFIIDPNGVVKHLSVNDLPVGRSVEETLRLVKAFQFVETHGEVCPANWTPESPTIKPSPTASKEYFEKVHQ lrrk2-prdx3-5 1433139 n/a 1433147 enzyme target enzyme target substrate substrate unspecified role unspecified role P30048:p.Thr146Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 1433148 enzyme enzyme unspecified role unspecified role Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind range range range range false gfp_or_myc tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells physical association physical association false false false 2B/C, supp 2 lrrk2-prdx3-7 1433139 n/a 1433147 enzyme target enzyme target substrate substrate unspecified role unspecified role n/a 1433148 enzyme enzyme unspecified role unspecified role gfp_or_myc tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind range range range range false human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells n/a 1433147 competitor competitor competitor unspecified role unspecified role spt_146 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 2D, supp 2 lrrk2-1 1433139 n/a 1433148 putative self putative self putative self putative self Q5S007:p.Gly2019Ser mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind range range range range false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells physical association physical association false true false 2Bi lrrk2-prdx3-1 1433140 n/a 1433147 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1433148 unspecified role unspecified role bait bait kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 1A prdx3-lrrk2 1433140 n/a 1433148 unspecified role unspecified role prey prey n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range false in vitro In vitro n/a 1433147 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n6-biotinyl-lysine biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 1B lrrk2-prdx3-2 1433141 n/a 1433147 unspecified role unspecified role prey prey n/a 1433148 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1C lrrk2-prdx3-8 1433141 n/a 1433148 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false Q5S007:p.[Gly2019Val;Ile2020Phe] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1433147 unspecified role unspecified role prey prey physical association physical association false false false Supp 1C lrrk2-prdx3-3 1433142 n/a 1433147 unspecified role unspecified role prey prey n/a 1433148 unspecified role unspecified role bait bait physical association physical association false false false 1D prdx3-lrrk2-1 1433142 n/a 1433147 unspecified role unspecified role bait bait n/a 1433148 unspecified role unspecified role prey prey physical association physical association false false false 1D lrrk2-prdx3 1433143 n/a 1433147 unspecified role unspecified role prey prey n/a 1433148 unspecified role unspecified role bait bait kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false no figure hydrogen_perox-prdx3 1433144 n/a 1433149 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1433147 enzyme enzyme unspecified role unspecified role purified purified human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells redox reaction oxidoreductase activity electron transfer reaction redox reaction false false false 4A peroxidase activity was higher in PRDX3 fraction than the phosphorylated PRDX3 fraction. lrrk2-prdx3-9 1433145 n/a 1433147 unspecified role unspecified role neutral component neutral component n/a 1433148 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind range range range range false colocalization colocalization false false false Supp 1D lrrk2-prdx3-10 1433146 n/a 1433148 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind range range range range false n/a 1433150 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 1D